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Method for extracting and purifying oleanolic acid from grape skins

A technology of oleanolic acid and grape skins, applied in the direction of organic chemistry, steroids, etc., can solve the problems of large loss of oleanolic acid, cost reduction, environmental pollution, etc., and achieve the reduction of heavy metal content, less loss of active ingredients, The effect of reducing production costs

Active Publication Date: 2015-11-18
天津市尖峰天然产物研究开发有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0008] Chinese patent CN1346653A extracts extract or oleanolic acid from grape skins, using supercritical extractor for extraction, alcohol dissolution, acid-base treatment, and activated carbon decolorization, which is not conducive to the removal of heavy metals, and the loss of oleanolic acid is relatively large Large, acid and alkali are easy to cause environmental pollution
In addition, supercritical extraction equipment is expensive, which is not conducive to reducing costs

Method used

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  • Method for extracting and purifying oleanolic acid from grape skins

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Effect test

Embodiment 1

[0024] A method for extracting and purifying oleanolic acid from grape skins, comprising the steps of:

[0025] (1) Wash the grape skins with deionized water and beat the pulp, use 70% ethanol aqueous solution as the extraction solvent, and use continuous countercurrent extraction at 50°C for 60 minutes, and the mass ratio of solid to liquid is 1:20, Filter to obtain grape skin extract;

[0026] (2) The obtained extract was concentrated under reduced pressure and evaporated ethanol, added 5 times the mass of deionized water to dissolve, stirred evenly, and put on the mixed resin column. The mixed resin was composed of cation exchange resin 001×8 with a mass ratio of 1:1 and macroporous resin AB-8 composition, wash with 6 times column volume of deionized water, 6 times column volume of 30% ethanol water solution, and finally wash with 7 times column volume of 95% volume percent ethanol solution Remove, collect the eluate with a percentage concentration of 95% ethanol aqueous s...

Embodiment 2

[0028] A method for extracting and purifying oleanolic acid from grape skins, comprising the steps of:

[0029] (1) Wash the grape skins with deionized water and make a pulp, use 80% ethanol aqueous solution as the extraction solvent, and use continuous countercurrent extraction at 50°C for 60 minutes, and the mass ratio of solid to liquid is 1:30, Filter to obtain grape skin extract;

[0030] (2) The obtained extract was concentrated under reduced pressure and the ethanol was evaporated. Add 5 times the mass of deionized water to dissolve, stir well, and put it on the mixed resin column. The mixed resin is composed of cation exchange resin D001FC with a mass ratio of 1:1 and macroporous resin HP- 40 composition, washed with 6 times column volume of deionized water, 6 times column volume of 30% ethanol aqueous solution, and finally eluted with 7 times column volume of 95% volume percent ethanol solution, Collect the eluate with a percentage concentration of 95% ethanol aqueou...

Embodiment 3

[0032] A method for extracting and purifying oleanolic acid from grape skins, comprising the steps of:

[0033] (1) Wash the grape skins with deionized water and beat them, use 100% ethanol aqueous solution as the extraction solvent, and use continuous countercurrent extraction at 50°C for 60 minutes, and the mass ratio of solid to liquid is 1:40. Filter to obtain grape skin extract;

[0034] (2) The obtained extract was concentrated under reduced pressure and evaporated ethanol, added 5 times the mass of deionized water to dissolve, stirred well, and put it on the mixed resin column. The mixed resin was composed of cation exchange resin D151 and macroporous resin AB- 8 composition, washed with 6 times column volume of deionized water, 6 times column volume of 30% ethanol aqueous solution, and finally eluted with 7 times column volume of 95% volume percent ethanol solution, Collect the eluate with a percentage concentration of 95% ethanol aqueous solution, concentrate under r...

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Abstract

The invention discloses a method for extracting and purifying oleanolic acid from grape skins. The method comprises the following steps: (1) beating the washed grape skins, using 60%-100% ethanol for continuous countercurrent extraction, filtering, Obtain grape skin extract; (2) evaporate ethanol, add water to dissolve, put on mixed resin column, and carry out elution, separation, purification and recrystallization. The method of the invention saves a large amount of solvents; reduces the content of heavy metals in products; has less loss of active ingredients, can greatly improve production efficiency, reduce production costs, and can make more full use of raw materials.

Description

technical field [0001] The invention belongs to a method for extracting and purifying oleanolic acid, in particular to a method for extracting and purifying oleanolic acid from grape skins. Background technique [0002] Oleanolic acid English name Oleanicacid, also known as soil angelica acid, belongs to pentacyclic triterpenoids, white needle crystal (ethanol); odorless, tasteless, soluble in methanol, ethanol, benzene, ether, acetone and chloroform, almost Insoluble in water, unstable to acid and alkali. Melting point: 308°C to 310°C, [α]20D+68° to +78° (C=0.15, chloroform). Oleanolic acid mainly has the effects of protecting the liver and reducing enzymes, promoting liver cell regeneration, anti-inflammation, strengthening the heart, diuresis, and anti-tumor. It also has the effects of lowering blood sugar, blood fat, and sedation. Active ingredients. [0003] [0004] Oleanolic acid structure [0005] The common extraction sources of oleanolic acid are the leaves ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C07J63/00
Inventor 吴巍
Owner 天津市尖峰天然产物研究开发有限公司