Unlock instant, AI-driven research and patent intelligence for your innovation.

Aeromonas and application of aeromonas in biotransformation of (R)-alpha-hydroxyphenylacetic acid

A hydroxyphenylacetic acid, Aeromonas guinea pig technology, applied in microorganism-based methods, biochemical equipment and methods, microorganisms, etc., can solve the problems of unavailability of S enantiomer, low yield, environmental pollution, etc. Low production cost, high efficiency and less pollution

Inactive Publication Date: 2013-11-27
JIMEI UNIV
View PDF3 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, this method not only has the disadvantages of low yield and expensive chiral resolving agent, but also the S enantiomer after resolution cannot be used, which will cause great pollution to the environment.

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Aeromonas and application of aeromonas in biotransformation of (R)-alpha-hydroxyphenylacetic acid
  • Aeromonas and application of aeromonas in biotransformation of (R)-alpha-hydroxyphenylacetic acid

Examples

Experimental program
Comparison scheme
Effect test

preparation example Construction

[0039]The application of Aeromonas caviae B18 in the asymmetric conversion of (R)-alpha-hydroxyphenylacetic acid is to prepare (R)-alpha-hydroxyphenylacetic acid, and the preparation method step is to transform Aeromonas caviae Caviae B18 is transferred to the transformation medium with an inoculum of 4% by volume, and the substrate acetophenone is added to the transformation medium, and the transformation culture is based on the conditions of 30-36°C and the shaker speed is 200r / min. For 19 hours, the pH of the transformation medium was 7.2, and the bacterial cells were removed by centrifugation to obtain (R)-α-hydroxyphenylacetic acid fermentation broth. Under the conditions of microwave vacuum drying for 1 h, the purity of (R)-α-hydroxyphenylacetic acid was 99.5%.

[0040] The preparation method of (R)-α-hydroxyphenylacetic acid transformation medium is as follows: starch 12.81g, casein peptone 12.74g, KH 2 PO 4 2.97g, K 2 HPO 4 1.2g, MgSO 4 0.15g, CaCl 2 0.125g, MnSO...

Embodiment

[0044] Screening of strains

[0045] Suspend 1g of pond bottom mud in 10mL of sterile water, carry out gradient dilution, and take 0.2mL of the diluted solution and spread it on a selective plate (including medium: KH 2 PO 4 1g, NaCl1g, K 2 HPO 4 1g, MgSO 4 0.15g, CaCl 2 0.125g, MnSO 4 0.015g, ZnSO 4 0.01g, substrate acetophenone acid 5mM, dilute to 1L with tap water, pH7.2). Add 50U / mL nystatin to the selective plate, incubate at 32°C for 2-3 days, observe the growth of the bacteria, and separate the bacteria group by streaking. Use a sterile toothpick to pick colonies of different shapes, and insert them into 2 mL of fermentation medium (starch 12.81 g, casein peptone 12.74 g, KH 2 PO 4 2.97g, K 2 HPO 4 1.2g, MgSO 4 0.15g, CaCl 2 0.125g, MnSO 4 0.015g, ZnSO 4 0.01g, pH7.2) in a test tube, cultured at 32°C, 200r / min shaker for 19h, added substrate acetophenone 5mM, transformed for 20h, and centrifuged the transformed fermentation broth at 10,000rpm for 10min, c...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention discloses aeromonas and an application of the aeromonas in biotransformation of (R)-alpha-hydroxyphenylacetic acid, and relates to the (R)-alpha-hydroxyphenylacetic acid. The invention further provides aeromonas caviae B18 and an application of the aeromonas caviae B18 in biotransformation of the (R)-alpha-hydroxyphenylacetic acid. The aeromonas caviae B18 is prepared through the following steps: adopting phenylglyoxylic acid as a substrate, carrying out treatments of preliminary screening, mutation, separation and purification to obtain the aeromonas caviae B18. With adopting the aeromonas caviae B18, the (R)-alpha-hydroxyphenylacetic acid can be selectively transformed. The method for preparing the (R)-alpha-hydroxyphenylacetic acid comprises: inoculating and transforming the aeromonas caviae B18 in a culture medium, then adding the phenylglyoxylic acid substrate; carrying out an asymmetric reduction reaction for the carbonyl positioned on the phenylglyoxylic acid substrate through a catalytic action; then separating and purifying the (R)-alpha-hydroxyphenylacetic acid reaction product from the fermentation broth. With the strain provided by the present invention, the transformed (R)-alpha-hydroxyphenylacetic acid has strong specificity and high yield, the process operation is simple, the cost is low, and the target product is easy to separate.

Description

technical field [0001] The invention relates to (R)-alpha-hydroxyphenylacetic acid, in particular to Aeromonas caviae B18 and its application in biological asymmetric transformation of (R)-alpha-hydroxyphenylacetic acid. Background technique [0002] (R)-α-Hydroxyphenylacetic acid is an important pharmaceutical intermediate, widely used in the synthesis of various drugs, such as penicillins, cephalosporins, anti-obesity drugs, anti-tumor agents, optically pure amino acids, coenzyme A , angiotensin-converting enzyme inhibitors, etc., can also be used to make chiral resolution reagents. At present, the demand for optically active α-hydroxyphenylacetic acid in the international market is growing at an average annual rate of more than 10%, and it has become an important pharmaceutical and fine chemical intermediate. [0003] Xin Meihua (Xin Meihua, Analytical Chemistry, 2001,29(8):990) reported a method for the preparation of α-hydroxyphenylacetic acid by direct resolution of c...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20C12P7/42C12R1/01
Inventor 李忠琴关瑞章
Owner JIMEI UNIV