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Method for improving freeze-drying survival rate of Lactobacillus acidophilus

A technology of Lactobacillus acidophilus and survival rate, applied in the direction of microorganism-based methods, biochemical equipment and methods, bacteria, etc., can solve the problems of low number of viable bacteria and low survival rate of freeze-drying

Inactive Publication Date: 2012-01-25
SHAANXI RES INST OF AGRI PRODS PROCESSING TECH +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] The object of the present invention is to provide a method for improving the freeze-drying survival rate of Lactobacillus acidophilus, which can solve the problems of low freeze-drying survival rate and small number of viable bacteria in the freeze-drying process of Lactobacillus acidophilus

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1-1

[0019] The MRS broth was sterilized at 118°C for 15 minutes, cooled to room temperature, then inoculated with Lactobacillus acidophilus at a 4% (V / V) inoculum, incubated at a constant temperature of 37°C for 18 hours, and then the culture solution was allowed to stand at 9°C for 1.0 h, take a sample to measure the number of viable bacteria, then centrifuge at 4°C and 7000rpm for 10min, discard the supernatant to obtain the Lactobacillus acidophilus sludge, weigh the sludge, and add skim milk with a weight of 20% (W / W) of the sludge powder, then add pH 6.8 phosphate buffer with 80% (W / W) weight of the sludge, then pre-freeze at -40°C for 8 hours, and then place it in a freeze dryer for freeze-drying, 4-6Pa, -50 After freeze-drying at ℃ for 24 hours, Lactobacillus acidophilus bacteria powder can be obtained, and its freeze-drying survival rate is 70.45% (46.11% for the control group), and the number of live bacteria is 2.1×10 11 cfu / g ((control group is 1.10×10 11 cfu / g)).

Embodiment 1-2

[0021] The MRS broth was sterilized at 118°C for 15 minutes, cooled to room temperature, then inoculated with Lactobacillus acidophilus at a 4% (V / V) inoculum, incubated at a constant temperature of 37°C for 18 hours, and then the culture solution was allowed to stand at 8°C for 0.5 h, take a sample to measure the number of viable bacteria, then centrifuge at 4°C and 7000rpm for 15min, discard the supernatant to obtain Lactobacillus acidophilus sludge, weigh the sludge, add skim milk with a weight of 10% (W / W) of the sludge powder, then add pH 6.8 phosphate buffer solution with 90% (W / W) weight of the sludge, then pre-freeze at -40°C for 12h, and then freeze-dry in a freeze dryer at 6Pa, -60°C Freeze-dry for 21 hours to obtain Lactobacillus acidophilus powder.

Embodiment 1-3

[0023] The MRS broth was sterilized at 118°C for 15 minutes, cooled to room temperature, then inoculated with Lactobacillus acidophilus at a 4% (V / V) inoculum, incubated at a constant temperature of 37°C for 18 hours, and then the culture solution was allowed to stand at 10°C for 1.5 h, take a sample to measure the number of viable bacteria, then centrifuge at 4°C and 7000rpm for 11min, discard the supernatant to obtain Lactobacillus acidophilus sludge, weigh the sludge, add skim milk with a weight of 16% (W / W) of the sludge powder, then add pH 6.8 phosphate buffer with 84% (W / W) weight of the sludge, then pre-freeze at -40°C for 11h, and then freeze-dry in a freeze dryer at 4Pa, -52°C Freeze-dry for 18 hours to obtain Lactobacillus acidophilus powder.

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Abstract

The invention aims to provide a method for improving the freeze-drying survival rate of Lactobacillus acidophilus. The method comprises the following steps of: culturing the Lactobacillus acidophilus for 18h at the temperature of 37 DEG C, performing sublethal treatment on the Lactobacillus acidophilus, centrifuging for 10 to 15min at the temperature of 4 DEG C at the rotating speed of 7,000rpm, adding skimmed milk and a phosphate buffer solution into bacterial sludge of the Lactobacillus acidophilus, pre-freezing for 8 to 12h at the temperature of -40 DEG C, and putting into a freeze dryer and freeze-drying for 18 to 24h at the temperature of between -50 and -60 DEG C under the vacuum degree of between 4 and 6Pa to obtain bacterial powder of the Lactobacillus acidophilus. In the method for improving the freeze-drying survival rate of the Lactobacillus acidophilus, a Lactobacillus acidophilus culture solution is subjected to cold shock, heat shock or acid shock treatment first so as to ensure that the Lactobacillus acidophilus induces to synthesize cold shock protein and heat shock protein under adverse circumstances or changes the composition of fatty acid of cell membranes, so that the resistance of the Lactobacillus acidophilus in the subsequent freeze-drying process is improved, and the freeze-drying survival rate and the viable count of the bacterial powder are improved.

Description

technical field [0001] The invention belongs to the technical field of production of Lactobacillus acidophilus products, and relates to a method for improving the freeze-dried survival rate of Lactobacillus acidophilus. Background technique [0002] Lactobacillus acidophilus (Lactobacillus acidophilus) belongs to the Lactobacillus genus in the Lactobacillus family. chain arrangement. No flagella, no movement, Gram stain positive, facultative anaerobic, naturally present in the gastrointestinal tract of humans and animals, human oral cavity, vagina and traditional fermented milk. The optimum growth temperature is 35°C-38°C. It does not grow below 20°C. The maximum growth temperature is 43°C-48°C. It has poor heat resistance and can use glucose, fructose, lactose, and sucrose for homozygous fermentation to produce DL-type lactic acid. [0003] Lactobacillus acidophilus is one of the commonly used probiotics in dairy products and their preparations. It can relieve lactose int...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20C12N1/04C12R1/23
Inventor 舒国伟王娟陈合马齐秦涛张华张强
Owner SHAANXI RES INST OF AGRI PRODS PROCESSING TECH
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