Processing method for efficiently removing deoxynivalenol (DON) from wheat grains

A technology for deoxynivalenol and wheat grains, which is applied in the field of processing to efficiently remove deoxynivalenol from wheat grains, and can solve problems such as safety concerns, damage to food nutrients, and ineffective detoxification

Inactive Publication Date: 2012-02-01
INST OF AGRO FOOD SCI & TECH CHINESE ACADEMY OF AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The detoxification effect of these chemical processes is good, but the safety problems that arise after processing are also worrying
At the same time, high temperature will destroy other nutrients in food, so it should not be used as the main means of detoxification

Method used

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  • Processing method for efficiently removing deoxynivalenol (DON) from wheat grains
  • Processing method for efficiently removing deoxynivalenol (DON) from wheat grains
  • Processing method for efficiently removing deoxynivalenol (DON) from wheat grains

Examples

Experimental program
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Effect test

Embodiment 1

[0039] Embodiment 1, remove the deoxynivalenol (DON) toxin in wheat grain

[0040] The method for extracting and detecting DON toxin content in wheat grain is as follows:

[0041] A) Extraction of DON

[0042] Grind the dried wheat grain sample (collected from the southern Jianghuai wheat producing area of ​​my country) polluted by DON toxin with a pulverizer, weigh 5 g accurately, place it in a 100 mL Erlenmeyer flask, add 35 mL of acetonitrile / water solution (84:16 , V / V), vibrated with a disperser for 3 minutes, mixed evenly, poured into a volumetric flask, added acetonitrile / water solution (84:16, V / V) to make the volume to 50mL, folded and filtered all the sample liquid with qualitative filter paper, and obtained supernatant.

[0043] Take 4mL of the supernatant, pass through the Bond Elut Mycotoxin purification column, accurately draw 3mL of the purification solution, transfer it to a graduated test tube, blow in nitrogen slowly with a nitrogen purging instrument at 50°C...

Embodiment 2

[0059] Embodiment 2, remove the deoxynivalenol (DON) toxin in wheat grain

[0060] Take by weighing the same wheat grain 1000g contaminated by DON toxin as in Example 1, and use the same method in Example 1 to detect that the DON toxin content in the wheat grain is 9.21 μg / g; according to NY / T 1094.1-2006 And NY / T 1094.5-2006, using Brabender experimental grinding Quadrumat (Quadruplex), to obtain 466.28g flour. The same method as in Example 1 was used to detect that the DON toxin content in the flour was 7.33 μg / g. Take 100g of flour, and add 52ml of 1g / L sodium chloride aqueous solution to it to make dough, wash the dough with a suitable flow rate, and knead the dough continuously at the same time. And continue to rinse and knead the formed gluten balls with tap water until the starch in the gluten is washed. The criterion for the washing of the starch in the gluten is: after adding potassium iodide / iodine solution to the water squeezed from the gluten, The color of the wa...

Embodiment 3

[0062] Embodiment 3, remove the deoxynivalenol (DON) toxin in wheat grain

[0063] Take by weighing the same wheat grain 1000g contaminated by DON toxin as in Example 1, and use the same method in Example 1 to detect that the DON toxin content in the wheat grain is 9.21 μg / g; according to NY / T 1094.1-2006 And NY / T 1094.5-2006, using Brabender experimental grinding Quadrumat (Quadruplex), to obtain 477.76g flour. The same method as in Example 1 was used to detect that the DON toxin content in the flour was 7.33 μg / g. Take 100g of flour, add 46ml of drinking water to it, and after making dough, wash the dough with an appropriate flow rate while kneading the dough continuously. And continue to rinse and knead the formed gluten balls with tap water until the starch in the gluten is washed away. The judgment standard for washing the starch in the gluten is: the water squeezed out from the gluten is colorless, drained and pressed to dry. To obtain wet gluten, dry the wet gluten at...

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Abstract

The invention discloses a processing method for efficiently removing deoxynivalenol (DON) from wheat grains. The method for removing the DON from the DON-polluted wheat grains comprises the following steps of: making the DON-polluted wheat grains into flour, and extracting gluten from the flour to obtain the gluten without the DON; and then removing the DON from the DON-polluted wheat grains. In the detoxicated gluten obtained by the processing method, no DON toxin is detected so as to fully meet the national standard requirements. The processing method has the beneficial effects that the DON can be removed from the wheat grains by simple processing and extraction technologies without damage on the quality of the original product as well as secondary pollution; and the produced detoxicated gluten can be taken as a semi-finished product to be reprocessed.

Description

technical field [0001] The invention relates to a processing method for efficiently removing deoxynivalenol from wheat grains. Background technique [0002] Deoxynivalenol (DON) is a trichothecene toxin mainly produced by Fusarium graminearum and Fusarium pink. These fungi grow in wheat, produce a large amount of DON toxin, contaminate wheat, and cause scab, a common disease of wheat. Trichothecenes are identified as the most dangerous naturally occurring food pollutants, and DON-contaminated food (wheat, corn, barley, etc.) is the most common, with strong stability, strong cytotoxicity and genotoxicity, After humans and other animals ingest the contaminated grains, they will cause acute poisoning symptoms such as anorexia, vomiting, diarrhea, fever, unsteady standing and unresponsiveness, which seriously endanger the safety of humans and animals. Especially in Jianghuai and other major southern wheat-producing areas of China, the climate of warm and rainy wheat from flowe...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A23J1/12
Inventor 刘阳陈飞邢福国
Owner INST OF AGRO FOOD SCI & TECH CHINESE ACADEMY OF AGRI SCI
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