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Preparation method of fluorescein sodium solution containing nano-sized metal particles

A technology of metal nanoparticles and sodium fluorescein, which is applied in the field of fluorescent materials, can solve problems such as difficult detection and weak luminous intensity of sodium fluorescein, and achieve the effects of ensuring sensitivity, enhancing luminous intensity, and enhancing luminous intensity

Inactive Publication Date: 2012-02-01
OCEANS KING LIGHTING SCI&TECH CO LTD +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0003] The purpose of the embodiments of the present invention is to provide a method for preparing a sodium fluorescein solution containing metal nanoparticles, which solves the technical problem that the luminescence intensity of sodium fluorescein is weak at low concentrations and brings difficulties to detection

Method used

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  • Preparation method of fluorescein sodium solution containing nano-sized metal particles
  • Preparation method of fluorescein sodium solution containing nano-sized metal particles
  • Preparation method of fluorescein sodium solution containing nano-sized metal particles

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preparation example Construction

[0014] In the preparation method of the embodiment of the present invention, metal nanoparticles are prepared through the reduction of ascorbic acid, and the metal nanoparticles are used to enhance the luminous intensity of fluorescein sodium, which achieves the effect of significantly enhancing the luminous intensity of fluorescein sodium and ensures the fluorescence. Sodium fluorescein has strong luminous intensity at low concentration, which ensures the sensitivity of low concentration sodium fluorescein in detection.

[0015] The embodiment of the present invention provides a method for preparing a sodium fluorescein solution containing metal nanoparticles, comprising the following steps:

[0016] Prepare a mixed solution of a metal compound and an organic additive, and an alkali metal borohydride solution, the concentration of the metal compound is below 0.5mmol / L, and mix the alkali metal borohydride solution, the metal compound, and the organic additive The solutions ar...

Embodiment 1

[0038] The embodiment of the present invention provides a method for preparing a sodium fluorescein solution containing metal nanoparticles, specifically as follows:

[0039] Preparation of silver seed sol: prepare 20 mL of an aqueous solution containing 0.5 mmol / L silver nitrate and 0.5 mmol / L sodium citrate;

[0040] The preparation concentration is the sodium borohydride aqueous solution of 0.1mol / L;

[0041] Quickly add 0.36mL of sodium borohydride aqueous solution to the silver nitrate mixed solution according to the ratio of the amount of sodium borohydride to silver nitrate of 3.6:1, and continue magnetic stirring for 10min, then age at room temperature for 10h to obtain silver crystals kind of sol;

[0042] Preparation of silver nanoparticle sol: Dissolve 3.4mg silver nitrate in 18.5mL deionized water, and make 1×10 -3g / mL Add 0.0185g CTAB to silver nitrate aqueous solution;

[0043] Add 0.5mL silver seed crystal sol to silver nitrate aqueous solution according to t...

Embodiment 2

[0051] The embodiment of the present invention provides a method for preparing a sodium fluorescein solution containing metal nanoparticles, specifically as follows:

[0052] Preparation of gold seed sol: prepare 20mL of aqueous solution containing 0.1mmol / L chloroauric acid and 0.05mmol / L PVP;

[0053] The preparation concentration is the sodium borohydride aqueous solution of 0.1mol / L;

[0054] Quickly add 0.3mL sodium borohydride aqueous solution to the mixed solution of chloroauric acid according to the ratio of the amount of sodium borohydride and chloroauric acid being 15:1, and continue magnetic stirring for 15min, then age at room temperature for 12h to obtain Gold seed sol;

[0055] Preparation of gold nanoparticle sol: Dissolve 4.12mg of chloroauric acid in 17mL of deionized water, and make 5×10 -3 g / mL Add 0.085g CTAB to the chloroauric acid aqueous solution;

[0056] Add 2 mL of gold seed sol to the aqueous auric acid chloride solution at a ratio of 1:8.5 by vol...

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Abstract

The invention belongs to the technical field of fluorescent materials, and provides a preparation method of fluorescein sodium solution containing nano-sized metal particles. The method comprises steps for preparing metal crystal seed sol, steps for preparing nano-sized metal particle sol, and steps for preparing the fluorescein sodium solution containing the nano-sized metal particles. According to the method provided by the invention, the nano-sized metal particle sol is prepared by using ascorbic acid; the nano-sized metal particle sol is added to a fluorescein sodium solution according to a preset amount, such that the luminous intensity of fluorescein sodium under a low concentration is greatly improved, and detection accuracy is ensured when low-concentration fluorescein sodium is adopted.

Description

technical field [0001] The invention belongs to the technical field of fluorescent materials, and in particular relates to a method for preparing a sodium fluorescein solution containing metal nanoparticles. Background technique [0002] The chemical name of sodium fluorescein is 9-(o-hydroxyphenyl)-6-hydroxy-3H-xanthene-3-one disodium salt, which has a high absorption coefficient and suitable quantum yield, and is one of the fluorescent probes. One of the most widely used fluorescent materials. However, due to the self-quenching phenomenon of fluorescein sodium in the resonance energy transfer process, its fluorescence intensity will gradually decrease with the increase of label concentration during application, so it needs to be controlled in a lower concentration range. , and the low sensitivity of the detection is caused by the weak fluorescence intensity, which brings difficulties to the detection. Contents of the invention [0003] The purpose of the embodiments of...

Claims

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Application Information

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IPC IPC(8): C09K11/06B22F9/24
Inventor 周明杰陆树新马文波
Owner OCEANS KING LIGHTING SCI&TECH CO LTD
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