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Culture method for accumulating organic iodine in Dunaliella salina

A breeding method, the technology of Dunaliella salina, applied in the biological field, can solve the problems of no consideration of iodine accumulation, few functions of salina powder, and low added value, so as to increase output and added value, easy to control breeding conditions, and add value to products. high value effect

Inactive Publication Date: 2012-02-15
TIANJIN UNIVERSITY OF SCIENCE AND TECHNOLOGY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The method is simple to operate, does not require high equipment and technology, and is convenient to control, but only considers the growth of Dunaliella salina and the accumulation of β-carotene, and does not consider the accumulation of iodine, and the produced salina powder has few functions. Value is not high

Method used

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  • Culture method for accumulating organic iodine in Dunaliella salina
  • Culture method for accumulating organic iodine in Dunaliella salina

Examples

Experimental program
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Effect test

Embodiment 1

[0025] A culture method for Dunaliella salina, the culture process is divided into two stages, the specific steps are as follows:

[0026] (1) Preliminary preparation: After mixing brine (salt content 14%) and fresh water at a ratio of 3:1, add the following substances in proportion in turn: KNO 3 600mg / L; KH 2 PO 4 60mg / L; NaHCO 3 500mg / L; Na 2 EDTA 1.89mg / L; FeCl 3 ·6H 2 O 2.44mg / L; H 3 BO 3 0.60mg / L; ZnCl 2 0.06mg / L; CoCl 2 ·6H 2 O 0.050mg / L; MnCl 2 4H 2 O 0.040mg / L; (NH 4 ) 6 Mo 7 o 24 4H 2 O 0.38mg / L; CuSO 4 ·5H 2 O 0.06mg / L. After mixing, adjust the pH to 8.0 to make a culture medium. Afterwards, the culture medium is introduced into the disinfection tank for disinfection, and after microscopic inspection without other biological contamination, it is imported into the algae cell culture tank; the algae species are introduced from the seed preservation room for expanded cultivation, so as to ensure that there is sufficient biomass for inoculation....

Embodiment 2

[0033] A culture method for Dunaliella salina, the culture process is divided into two stages, the specific steps are as follows:

[0034] (1) Preliminary preparation: After mixing brine (salt content 14%) and fresh water at a ratio of 3:1, add the following substances in proportion in turn: KNO 3 600mg / L; KH 2 PO 4 60mg / L; NaHCO 3 500mg / L; Na 2 EDTA 1.89mg / L; FeCl 3 ·6H 2 O 2.44mg / L; H 3 BO 3 0.60mg / L; ZnCl 2 0.06mg / L; CoCl 2 ·6H 2 O 0.050mg / L; MnCl 2 4H 2 O 0.040mg / L; (NH 4 ) 6 Mo 7 o 24 4H 2 O 0.38mg / L; CuSO 4 ·5H 2 O 0.06mg / L. After mixing, adjust the pH to 8.0 to make a culture medium. Afterwards, the culture medium is introduced into the disinfection tank for disinfection, and after microscopic inspection without other biological contamination, it is imported into the algae cell culture tank; the algae species are introduced from the seed preservation room for expanded cultivation, so as to ensure that there is sufficient biomass for inoculation....

Embodiment 3

[0040] A culture method for Dunaliella salina, the culture process is divided into two stages, the specific steps are as follows:

[0041] (1) Preliminary preparation: After mixing brine (salt content 14%) and fresh water at a ratio of 3:1, add the following substances in proportion in turn: KNO 3 600mg / L; KH 2 PO 4 60mg / L; NaHCO 3 500mg / L; Na 2 EDTA 1.89mg / L; FeCl 3 ·6H 2 O 2.44mg / L; H 3 BO 3 0.60mg / L; ZnCl 2 0.06mg / L; CoCl 2 ·6H 2 O 0.050mg / L; MnCl 2 4H 2 O 0.040mg / L; (NH 4 ) 6 Mo 7 o 24 4H 2 O 0.38mg / L; CuSO 4 ·5H 2 O 0.06mg / L. After mixing, adjust the pH to 8.0 to make a culture medium. Afterwards, the culture medium is introduced into the disinfection tank for disinfection, and after microscopic inspection without other biological contamination, it is imported into the algae cell culture tank; the algae species are introduced from the seed preservation room for expanded cultivation, so as to ensure that there is sufficient biomass for inoculation....

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Abstract

A culture method for accumulating organic iodine in Dunaliella salina achieves the effects of promoting the growth of the Dunaliella salina and increasing the cumulant of Beta-carotene and organic iodine by changing Dunaliella salina culture conditions. The biomass and Beta-carotene cumulant of the finally obtained Dunaliella salina are more than 1.5 times as much as the biomass and Beta-carotene cumulant of the conventionally cultured Dunaliella salina, and the content of the organic iodine in the alga reaches 110.6mg / kg by dry weight. The culture method shortens the Dunaliella salina culture time, the culture conditions can be easily controlled, the production cost is low, and the added value of products is high. Substances added in the whole culture process come up to national regulations, so the product is safe and reliable, and can be applied in food and pharmaceutical industries.

Description

technical field [0001] The invention belongs to the field of biotechnology, and in particular relates to a cultivation method for Dunaliella salina. Background technique [0002] Dunaliella salina belongs to Chlorophyta, Volvocales, Poly-blepharidaceae, Dunaliella, Teodoresco. The grown unicellular green algae without cell wall has been widely used in food, medicine, health care, chemical industry and aquaculture industry. This algae has been industrialized in countries such as Australia, the United States and Israel. Dunaliella salina can accumulate glycerol and β-carotene under specific culture conditions, and the accumulation of β-carotene can reach 14% of the dry weight, ranking first among all organisms in nature. β-carotene, as a functional nutrition enhancer with excellent performance, has the functions of anti-radiation, regulating human immunity, inhibiting tumors, preventing and treating cardiovascular diseases, and improving vision. It has a wide range of applic...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/12C12R1/89
Inventor 徐仰仓洪利亚
Owner TIANJIN UNIVERSITY OF SCIENCE AND TECHNOLOGY
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