Method for extracting polysaccharide from imperata rhizome

Abstract

The invention discloses a method for extracting polysaccharide from imperata rhizome, which belongs to the biology technical field. The method comprises the following steps: segmentizing imperata rhizome and adding water for wetting, placing into a steam explosion reaction pot, introducing steam and carrying out a steam explosion treatment for 10-60 seconds under the pressure condition of 1-10MPa, collecting a steam exploded substrate and adding water for reflux extraction for 1-2 hours, adding protease in an extract for enzymatic hydrolysis, filtering an enzymatic hydrolysate and performing ultrafiltration by an ultrafilter membrane, collecting a permeate and concentrating by the ultrafilter membrane, collecting a concentrate and carrying out alcohol precipitation, vacuum drying to obtain imperata rhizome polysaccharide. The method of the invention has the advantages of simple operation, high recovery rate, low energy consumption, small pollution and easy industrial production.

Description

technical field

[0001] The invention belongs to the field of biotechnology, and in particular relates to a method for extracting polysaccharides from the roots of Imperata coronata. Background technique

[0002] Imperata cylindrica is the rhizome of the perennial grass Imperata cylindrica (scientific name: Imperata cylindrica). Traditional Chinese medicine believes that the rhizome of Imperatae is sweet and cold in nature and has the effects of cooling blood, stopping bleeding, clearing away heat and diuresis. Modern research has found that the polysaccharides of Imperatae Rhizome have the functions of improving human immunity and anti-tumor.

[0003] Existing methods for extracting polysaccharides from Imperata coronata generally use water extraction, microwave or ultrasonic assisted extraction, and there are also enzymatic water extraction methods, followed by alcohol precipitation to remove protein. For example, Gou Jiangang et al. used the extraction temperature of 80°...