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Bacillus microecological preparation and liquid-solid fermentation combining preparation process thereof

A micro-ecological preparation and preparation process technology, applied in the directions of microorganisms, microorganism-based methods, biochemical equipment and methods, etc., can solve the problems of easily polluted bacteria, unstable number of viable bacteria, etc., and achieve no three wastes and energy consumption. The effect of decreasing, stable and controllable product quality

Inactive Publication Date: 2014-01-22
天津市育琪生物技术有限公司 +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] The object of the invention is: aiming at defects such as unstable number of live bacteria and easy contamination of miscellaneous bacteria in the existing technical process for preparing bacillus probiotics, propose a new process for preparing bacillus probiotics

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0023] Embodiment 1: liquid-solid combined fermentation process of Bacillus licheniformis probiotics.

[0024] Inoculate Bacillus subtilis on a solid LB medium plate, culture at 37°C for 24 hours, pick a single colony and inoculate it in a liquid seed medium (1.6% peptone, 1.0% yeast powder, 0.5% NaCl, pH 6.0-7.0 ), cultivated on a rotary shaker at 200 rpm at 37°C for 16 hours to obtain a primary seed liquid. Fill a 1L Erlenmeyer flask with 20% volume of liquid seed medium, inoculate 1% of the above-mentioned primary seed solution, culture at 37°C, 200 rpm, and ferment for 16 hours to obtain a secondary seed solution. Fill a 10L fermenter with 50% volume of liquid medium, inoculate 1% of the above-mentioned secondary seed solution, and cultivate at 37°C with a stirring speed of 200rpm for 6-8 hours, then start feeding feeding solution at a flow rate of 50mL / min, and ferment to 17~24h, stop fermentation. The bran and the grape seed extract with a mass ratio of 1%~2% are dried...

Embodiment 2

[0025] Embodiment 2: liquid-solid combined fermentation process of Bacillus subtilis probiotics.

[0026] Inoculate Bacillus licheniformis on a solid LB medium plate, culture at 37°C for 24 hours, pick a single colony and inoculate it in a liquid seed medium (1.6% peptone, 1.0% yeast powder, 0.5% NaCl, pH 6.0-7.0 ), cultivated on a rotary shaker at 200 rpm at 37°C for 16 hours to obtain a primary seed solution. Fill a 1L Erlenmeyer flask with 20% volume of liquid seed medium, inoculate 1% of the above-mentioned primary seed solution, culture at 37°C, 200 rpm, and ferment for 16 hours to obtain a secondary seed solution. Fill a 10L fermenter with 50% volume of liquid medium, inoculate 1% of the above-mentioned secondary seed solution, and cultivate at 37°C with a stirring speed of 200rpm for 6-8 hours, then start feeding feeding solution at a flow rate of 50mL / min, and ferment to 17~24h, stop fermentation. The bran and the grape seed extract with a mass ratio of 1%~2% are dri...

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Abstract

The invention provides a microbial feed additive and a preparation method thereof. The main processes are as follows: respectively culturing bacillus subtilis and bacillus licheniformis for 17-24 hours at 35-37 DEG C by using liquid media; mixing the bacillus subtilis and bacillus licheniformis with solid media which are sterilized and contain bran and grape seed extracts uniformly, and inoculating the bacillus subtilis and bacillus licheniformis into the solid media; then carrying out short solid fermentation (1-3 hours) at 35-37 DEG C; by utilizing the characteristic that bacillus is easy to generate spores when the environment changes, enabling the metabolically active bacterium to rapidly ripen and generate spores after short solid fermentation; and then grinding and packaging to prepare the microbial feed additive. The effective bacterial count of the microbial feed additive is not less than 1*108CFU / g. 0.1% of microbial feed additive is added to the feed for livestock and poultry to be used for reducing the rate of livestock and poultry diarrhea, reducing the infection rate, improving the feed intake and improving the daily gain.

Description

technical field [0001] The invention relates to a micro-ecological preparation mainly composed of bacillus subtilis and bacillus licheniformis, and also relates to a liquid-solid combined fermentation preparation process of the micro-ecological preparation. Background technique [0002] Micro-ecological preparations are made from beneficial microorganisms through cultivation, fermentation, drying, processing and other processes to produce live bacteria or preparations containing bacteria and their metabolites, which have functions such as disease resistance, disease treatment, and growth promotion. Moreover, it has the characteristics of no residual pollution, no side effects and no drug resistance. Bacillus subtilis and Bacillus licheniformis are ideal strains for probiotics, and they are included in the feed-grade microbial additives that can be directly fed to animals announced by the Ministry of Agriculture of my country. They are acid-resistant, alkali-resistant, high-...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20A23K1/16C12R1/125C12R1/10
Inventor 刘晋生孙骏跃宋诙谭明李丽刘阳郑雯徐健勇吴雅倩
Owner 天津市育琪生物技术有限公司
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