Culture medium and culture method for long-term and continuous culture of Boveria Stevens

A culture medium and long-term technology, applied in fish farming, application, animal husbandry, etc., can solve the problems of endangering the healthy and sustainable development of the sea cucumber industry, affecting the growth and development of sea cucumbers, and the death of sea cucumbers, so as to achieve batch preparation and Application, stable and reliable sources of raw materials, low cost of cultivation

Active Publication Date: 2021-07-13
YELLOW SEA FISHERIES RES INST CHINESE ACAD OF FISHERIES SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In parasitic diseases, the infection of a deuterostomum parasitized on the respiratory tree of sea cucumbers can lead to decreased adhesion, reduced food intake, slow growth, and even visceral excretion reactions, which seriously affect the growth and development of sea cucumbers, and cause Its immunity is reduced, and it is susceptible to secondary infection by other microorganisms, leading to indiv

Method used

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  • Culture medium and culture method for long-term and continuous culture of Boveria Stevens
  • Culture medium and culture method for long-term and continuous culture of Boveria Stevens
  • Culture medium and culture method for long-term and continuous culture of Boveria Stevens

Examples

Experimental program
Comparison scheme
Effect test

Example Embodiment

[0020] Example 1: Comparison of the survival of the posterior insects under different medium conditions

[0021] The specific steps are as follows: After filtering sea water, boil disinfection, cool to 8 ~ 10 ° C, add yeast fermentation liquid concentrate to the final concentration of 2.5 mg / L, and add a subharidacillus CSDN-6, photosynthetic bacteria, and Bacillus to final concentration 10 5 CFU / mL, wherein group 1 is a sub-cheese Lactobacillus CSDN-6 + yeast fermentation fluid concentrate, group 2 is a photosynthetic bacterial + yeast fermentation liquid concentrate, group 3 is a Bacillus + yeast fermentation fluid concentrate. The above liquid is three medium of the outer pure cultivation of rear oral insects, and the medium of the body cavity liquid and the breathing tree tissue fragmentation in sterilization seawater is compared to the control.

[0022] In November 20020, there was a resect disease in Dalian a farm, and the sick thorn was taken back to the laboratory, and...

Example Embodiment

[0026] Example 2: Selection of Yeast Fermentation Liquid Concentrates

[0027] The specific steps are as follows: After filtering sea water, boil 5 to 10 min, then cool it to 8 ~ 10 ° C, add 0.1 mg / L, 1.0 mg / L, 2.5 mg / L, 5.0mg / L, 10.0mg / L, 25mg / L. The yeast fermentation fluid concentrate, adequately mixed, add a sub-cheese CSDN-6 to final concentration of 10 5 CFU / ml, the liquid is a medium having an outer pure culture of rear oral insects.

[0028] In December 2020, the stabbin sanctuary of the patients with aushagus was taken back to the laboratory to dissect the breathing tree to produce water dip, and the microscope observation has a large number of hillous worms in the breathing tree. The posterior worm separated from the sick stabbed breathing tree is removed under the microscope and other organisms and other organisms; that is, according to the result of the count under the microscope, according to the post-mouth density of 100 / ml After the rear sodus transf...

Example Embodiment

[0032] Example 3: Culture Salinity Control

[0033] The specific steps are as follows: By adding fresh water to the sea, the medium is adjusted to 0 ‰, 2.5 ‰, 5 ‰, 10 ‰, 15 ‰, 20 ‰, 25 ‰, 30 ‰, 35 ‰, 40 ‰, 45 ‰, add 2.5mg / L yeast fermentation fluid concentrate and 10 5 CFU / mL of the sub-cheese CSDN-6, which is a medium having an outer pure culture medium of rear oral.

[0034] In December 2020, the stabbin dry transport of the suffering of aushagus was taken back to the laboratory, and the breathing tree was used to make a water dip film. The microscope observation has a large number of post-hugs to breathe in the breathing tree. The posterior worm separated from the sick stabbed breathing tree is removed under the microscope and other organisms and other organisms; that is, according to the result of the count under the microscope, according to the post-mouth density of 100 / ml After the lathem is transferred to the medium, 40 ml of water body, pH 7.8 ~ 8.5, temperature 8 ~ ...

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Abstract

The invention relates to a culture medium and a culture method for long-term and continuous culture of Boveria Stevens, and belongs to the technical field of biology. The culture medium is liquid and contains yeast fermentation liquor concentrate with the final concentration of 1-3mg/L, lactobacillus paracasei CSDN-6 with the final concentration of 1 * 10 < 5 > CFU/ml and the balance of filtered and boiled natural seawater, wherein the salinity of the natural seawater is 28-32 per mill, and the pH is 7.8-8.5. The invention also provides a method for long-term and continuous culture of the Boveria Stevens by using the culture medium, the culture medium for the Boveria Stevens can be kept in a clear state for a long time in the culture process and does not have the phenomena of decay and peculiar smell, the Boveria Stevens can be continuously and stably cultured for a long time, and a technical support is provided for subsequent research on basic biology and drug screening of parasites.

Description

technical field [0001] The invention belongs to the field of biotechnology, and in particular relates to a long-term and continuous culture medium and a culture method of Deuterostomum. Background technique [0002] Apostichopus, also known as Apostichopus japonicus, has become a pillar industry of marine aquaculture in my country since the success of large-scale artificial propagation technology in the 1980s. In 2019, the national sea cucumber breeding area was about 246,745 hectares, the output reached 171,700 tons, the direct output value was more than 30 billion yuan, and the economic output value of the industrial chain was nearly 100 billion yuan. One (2020 Fishery Statistical Yearbook). [0003] With the gradual expansion of the sea cucumber industry, disease problems have become increasingly prominent, causing economic losses of more than 3 billion yuan each year. The reported causes of the disease include bacterial infection, fungal infection, and parasitic infecti...

Claims

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Application Information

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IPC IPC(8): A01K61/30
CPCA01K61/30
Inventor 王印庚王锦锦廖梅杰李彬畅孟阳尚晓金
Owner YELLOW SEA FISHERIES RES INST CHINESE ACAD OF FISHERIES SCI
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