Heterotrophic culture method for microalgae by utilizing starch and immobilized microorganism co-culture

A technology of immobilized microorganisms and heterotrophic culture, applied in microorganism-based methods, biochemical equipment and methods, microorganisms, etc., can solve problems such as increasing production costs, difficulty in directly utilizing starch, and difficulty in direct utilization of microorganisms, and achieves carbon reduction. effect of source cost

Inactive Publication Date: 2017-10-17
YANGZHOU UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Starch, as a kind of carbohydrate with wide sources and low price, is an ideal carbon source, but because it is difficult for microorganisms to directly utilize high-molecular starch, and many microorganisms including algae cannot secrete amylase, therefore, in many During the cultivation of microorganisms, it is difficult to directly use starch
At present, there are a few reports on the use of starch for microalgae heterotrophic / combinotrophic culture, but in the above studies, the starch needs to be hydrolyzed into glucose or oligosaccharides by chemical or enzymatic methods before being used in the culture of microalgae. The process increases the unit operation of the microalgae cultivation process, which in turn increases the production cost

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0021] (1) In a 2L airlift reactor, add the medium containing 80g / L wheat starch, sterilize and cool;

[0022] (2) Inoculate Chlorella ellipsoides cultivated to the logarithmic growth phase with an inoculum of 10% (v / v);

[0023] (3) After the membrane-coated yeast was cultured to the logarithmic phase, it was immobilized by calcium alginate embedding, and the immobilized microspheres were added to the culture medium with a yeast cell content of 5% of the dry weight of microalgae cells start cultivating in

[0024] (4) Cultivate under the conditions of 25°C, 2vvm ventilation and pH 7.0, and collect the algae liquid after the cultivation.

[0025] Processing effect test:

[0026] No leakage of yeast cells was found during the cultivation process, and the final concentration of microalgae reached 3.8g / L.

Embodiment 2

[0028] (1) In a 500mL Erlenmeyer flask, add a medium containing 100g / L cornstarch, sterilize and cool;

[0029] (2) Insert Nannochloropsis algae cultivated to logarithmic growth phase with an inoculum size of 20% (v / v);

[0030] (3) After Aspergillus niger is cultivated to the logarithmic phase, it is immobilized by carrageenan embedding, and the immobilized microspheres are added to the culture medium with a cell content of 20% of the dry weight of microalgae cells of Aspergillus niger to start culturing ;

[0031] (4) Cultivate under the conditions of 15°C, 15vvm ventilation and pH 9.5, and collect the algae liquid after the cultivation.

[0032] Processing effect test:

[0033] No leakage of Aspergillus niger cells was found during the cultivation process, and the final concentration of microalgae reached 42.2g / L.

Embodiment 3

[0035] (1) In a 1L bubble column reactor, add the medium containing 50g / L sweet potato starch, sterilize and cool;

[0036] (2) insert the Haematococcus pluvialis cultivated to the logarithmic growth phase with an inoculum of 5% (v / v);

[0037] (3) After the Escherichia coli engineering bacteria with starch hydrolysis activity were cultivated to the logarithmic phase, they were immobilized by glutaraldehyde crosslinking, and the immobilized microspheres were added with a bacterial content of 12% of the dry weight of microalgae cells Start culturing in the culture medium;

[0038] (4) Cultivate under the conditions of 30° C., 0.1 vvm ventilation and pH 6.5, and collect the algae liquid after the cultivation.

[0039] Processing effect test:

[0040] No leakage of bacterial cells was found during the cultivation process, and the final concentration of microalgae reached 24.1g / L.

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Abstract

The invention belongs to the biotechnology field and in particular relates to a heterotrophic culture method for microalgae by utilizing starch and immobilized microorganism co-culture. The method comprises the following steps: preparing a culture medium, inoculating, immobilizing microorganism cells, culturing, and collecting cells. The method provided by the invention has the main characteristics that co-culture is carried out on the microalgae and immobilized microorganisms with starch hydrolysis capability, the problem that the microalgae is difficult to be subjected to heterotrophic culture by utilizing a low-cost starch type raw material is solved, a new approach is provided for reducing carbon source cost of the heterotrophic culture of the microalgae, pure culture of the microalgae is realized by immobilizing co-culture microorganisms, and compared with the conventional co-culture technology, algae-bacteria separation is realized by virtue of simple filtration and purely cultured microalgae cells can be obtained finally, so that the method provided by the invention can be widely applicable to a production process of various algae-based biological products.

Description

technical field [0001] The invention specifically relates to a method for cultivating microalgae heterotrophically by starch through co-cultivation with immobilized microorganisms, and belongs to the field of biotechnology. Background technique [0002] The problem of light limitation in the autotrophic culture process of microalgae makes the design and operation of the photosynthetic reactor more difficult, and because the growth of cells is limited by light under autotrophic growth, the cell growth is usually slow, the culture period is long, and the final biological The lower biomass concentration will further increase the difficulty of the subsequent cell harvesting process. Some algae can grow heterotrophically using exogenous organic carbon sources under the condition of no light source. In heterotrophic culture, the addition of exogenous organic carbon sources can significantly promote the growth rate of cells in the microalgae culture process, greatly shortening the ...

Claims

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Application Information

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IPC IPC(8): C12N1/12C12N11/10C12N1/14C12N1/16C12R1/89C12R1/645C12R1/685C12R1/785
CPCC12N1/12C12N1/14C12N1/16C12N11/10
Inventor 王仕楷汪旭陶慧慧孙祥圣田永婷
Owner YANGZHOU UNIV
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