Method of heterotrophically culturing microalgae with sucrose by means of immobilized microorganism co-culture

Abstract

The invention belongs to the technical of biology and particularly relates to a method of heterotrophically culturing microalgae with sucrose by means of immobilized microorganism co-culture. The method includes the following steps: immobilization of microbial cells, preparation of a culture medium, successive inoculation of microalgae cells and microspheres containing the immobilized microorganisms on the culture medium, cultivation, and cell collection. According to the method, microorganisms, which have extracellular sucrase secretion capability, are immobilized and are co-cultured with the microalgae, so that not only is a problem that the microalgae is difficult to heterotrophically grow with sucrose solved, but also a problem of difficulty in separation of algae-bacteria in a co-culture technology in the prior art can be solved. The method effectively increases the utilization efficiency of the microalgae to the sucrose and achieves pure-cultivation of the microalgae, so that heterotrophic culture of the microalgae can be carried out with low-cost organic carbon sources. The method, meanwhile, achieves pure-cultivation of microalgae cells, so that the method can be widely applied to production of various algae-based bioproducts.

Description

technical field [0001] The invention specifically relates to a method for cultivating microalgae heterotrophically with sucrose through co-cultivation of immobilized microorganisms, and belongs to the field of biotechnology. Background technique [0002] As a kind of photoautotrophic microorganism, microalgae can use light energy to convert carbon dioxide and water into various chemical substances through photosynthesis, such as protein, fatty acid, vitamin, etc. Bioactive substances with high value, such as Haematococcus pluvialis can accumulate higher levels of astaxanthin, and spirulina can produce higher carotene. Based on the above characteristics, microalgae can be widely used in the production of animal and aquatic bait, food, medicine, health care products and food additives, etc., with broad application prospects and good economic value. [0003] All algae have photosynthetic ability. Therefore, microalgae usually adopt a photoautotrophic culture mode. Autotrophic ...

AI Technical Summary

Problems solved by technology

[0006] In the heterotrophic culture process of microalgae using sucrose as carbon source, aiming at the problem that it is difficult for microalgae to metabolize sucrose heterotrophically and the two are difficult to separate in the existing microalgae-yeast co-cultivation system, the present invention provides a Immobilized microbial co-cultivation is a method of cultivating microalgae heterotrophically using sucrose. First, immobilize microorganisms with the ability to secrete extracellular invertase, and then co-cultivate them with microalgae. In this system, microalgae can use immobilized The monosaccharide produced by degrading sucrose by degrading cells can effectively solve the problem that microalgae are difficult to use sucrose under sucrose heterotrophic culture. At the same time, the immobilization of cells makes the microalgae in the culture system in a pure culture state. After the culture, you can get Pure cultured microalgae cells can be widely used in the production process of various algae-based biological products