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Kit for detecting tubercle bacillus protein antigens, and preparation method thereof

The technology of a mycoprotein and kit is applied in the field of enzyme-linked immunosorbent assay kit for detecting tuberculosis protein antigen and its preparation, which can solve problems such as difficulty and low positive detection rate of tuberculosis, and achieve good sensitivity and specificity Effect

Inactive Publication Date: 2012-04-25
XIAMEN UNIV
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, since this type of diagnostic method is based on the fact that normal human body can produce a normal immune response to the invasion of tuberculosis bacteria and lead to the appearance of antibodies in the serum, it is effective for the infection of the elderly and children with immunodeficiency and low immunity, as well as the double infection of tuberculosis bacteria and AIDS. Infection often causes misdiagnosis or missed diagnosis, especially for extrapulmonary infection, such as tuberculous meningitis, bone tuberculosis and renal tuberculosis, etc., especially for children with tuberculous meningitis, the positive detection rate of tuberculosis in the cerebrospinal fluid is very low

Method used

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  • Kit for detecting tubercle bacillus protein antigens, and preparation method thereof
  • Kit for detecting tubercle bacillus protein antigens, and preparation method thereof
  • Kit for detecting tubercle bacillus protein antigens, and preparation method thereof

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Embodiment Construction

[0057] The following embodiments will further illustrate the present invention in conjunction with the accompanying drawings.

[0058] Figure 8 A schematic diagram of the structure and composition of the embodiment of the kit for detecting tuberculosis protein antigen of the present invention is given. The embodiment of the kit for detecting Mycobacterium tuberculosis protein (antigen) is provided with box body P, PBST lotion bottle 1, color development A liquid bottle 21, color development B liquid bottle 22, CFP10-ESAT6 protein standard sample bottle 3, PPD Protein standard sample bottle 4, sample diluent bottle 5, polystyrene enzyme-linked detection plate 6, 2M H 2 SO 4 Stop solution bottle 7, enzyme-labeled secondary antibody bottle 8, CFP10-ESAT6 polyclonal antibody bottle 91 and positive anti-tuberculosis bacterium polyclonal antibody bottle 92; the PBST lotion bottle 1, color development A liquid bottle 21, color development B Liquid bottle 22, CFP10-ESAT6 protein s...

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Abstract

The invention provides a kit for detecting tubercle bacillus protein antigens, and a preparation method of the kit, and relates to two tubercle bacillus protein antigens. The kit is provided with a kit body, a lead stearate (PBST) lotion bottle, a tetramethyl benzidine (TMB) chromogenic liquid bottle, a CFP10-ESAT6 protein standard sample bottle, a pure protein derivation (PPD) protein standard sample bottle, a sample diluent bottle, a polystyrene enzyme-linked detection board, a stop solution bottle, an enzyme-labeled secondary antibody bottle and an upper layer detection antibody bottle; and the preparation method of the kit comprises the steps of: preparing experiment reagent, catching the coating of antibody, closing and storing an enzyme-labeled board, and preparing the kit and other reagent samples. The enzyme-linked immuno sorbent assay (ELISA) kit which is used for detecting the tubercle bacillus protein antigens by a double-antibody sandwich method and the preparation method of the kit are rapid, simple and better in sensitivity and specificity, thus undoubtedly opening up new thinking and method for detecting the specific protein antigens of tuberculosis bacteria, providing auxiliary diagnosis for tuberculosis and protecting the population health. The kit becomes one of the diagnosis means of the tuberculosis, thus providing technical support for prevention and cure of the tuberculosis.

Description

technical field [0001] The invention relates to two tuberculosis protein antigens, in particular to an enzyme-linked immunosorbent assay (Enzyme-linked immunosorbent assay, ELISA) kit for detecting the tuberculosis protein antigen and a preparation method thereof. Background technique [0002] Tuberculosis has been listed as one of the major infectious diseases in my country. Nearly half of the people are tuberculosis carriers. The estimated number of patients is 4.5 million, and the annual incidence is about 1.3 million, accounting for about 16% of the global incidence. The reasons for the severe epidemic are related to the lagging of clinical diagnosis technology and new drug development, ineffective prevention and control, double infection of Mycobacterium tuberculosis and human immunodeficiency virus (HIV), and the sharp increase in the number of drug-resistant tuberculosis patients. [0003] At present, the existing tuberculosis ELISA detection kit is mainly used to det...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/569G01N33/558G01N21/31
Inventor 李晓彤于占娇杨赟
Owner XIAMEN UNIV
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