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Bio-control combined bacterium agent CB28 admixture for preventing cucumber downy mildew

A technology of cucumber downy mildew and compound bacterial agent, which is applied in the direction of biocides, bactericides, biocides, etc., can solve the problems of high cost, high pesticide residues, and difficult control of cucumber downy mildew, and achieve low price, Effect of cost saving and protection of farmland ecosystem

Inactive Publication Date: 2012-05-02
NANJING AGRICULTURAL UNIVERSITY
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0007] The purpose of the present invention is to provide a biocontrol compound bacterial agent for the prevention and treatment of cucumber downy mildew in order to solve the problem of difficulty in controlling cucumber downy mildew in agricultural production, high cost, increasingly prominent chemical pesticide resistance, and high pesticide residue by using biological control. CB28 mixture, the CB28 mixture has a control effect of more than 62.00% on cucumber downy mildew

Method used

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  • Bio-control combined bacterium agent CB28 admixture for preventing cucumber downy mildew
  • Bio-control combined bacterium agent CB28 admixture for preventing cucumber downy mildew
  • Bio-control combined bacterium agent CB28 admixture for preventing cucumber downy mildew

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Experimental program
Comparison scheme
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Embodiment 1

[0030] Strain source: BS13 was isolated from banana rhizosphere soil in Machong, Dongguan City, Guangdong Province; BJN15 was isolated from banana pseudostem in Machong, Dongguan City, Guangdong Province.

[0031] Strain Isolation Method: Plate Dilution Method (Microbiological Laboratory of Nanjing Soil Institute, Chinese Academy of Sciences. Soil Microbial Research Method [M]. Beijing: Science Press Du, 1985.)

[0032] Screening basis for strains: Determination of five enzyme activities in hydrolysis (protease, chitinase, cellulase, siderophilic, IAA) Yang J.-H., Liu H.~X., Zhu G.-M., Xu L. .-P., Pan Y.-L., and Guo J.-H.*.2008.Diversity analysis of antagonists from rice associated bacteria and their application in biocontrol of rice diseases.Journal of Applied Microbiology.104(1): 91~104.) and the determination of antagonistic activity against Phytophthora bacterium (confrontation culture method, Lin Fucheng, Li Debao. Bacillus subtilis (Bacillus subtilis) to the bacteriolysi...

Embodiment 2

[0039] A biocontrol complex bacterial agent CB28 mixture for controlling cucumber downy mildew, which contains two bacterial components: Bacillus megaterium BS13 (CGMCC NO.4734) and Enterobacter BJN15 (CGMCC NO.4733).

[0040] The preparation method of the biocontrol compound bacterial agent CB28 mixture of this control cucumber downy mildew is as follows:

[0041] (1) Cultivation of seed bacteria liquid: Inoculate Bacillus megaterium BS13 with the preservation number of CGMCC NO.4734 and Enterobacter BJN15 with the preservation number of CGMCC NO.4733 into the LB culture liquid respectively, and inoculate them at 30~32°C, 180~ Cultivate under the condition of 220rpm until the OD value at 600nm is between 0.5 and 0.8, and end the cultivation to obtain the seed bacterial liquid;

[0042] (2) Wet bacteria preparation: take the seed bacteria solution in step (1), add it to LB culture solution at a volume ratio of 1:250, vibrate at 30-32°C and 180-220rpm for 45-48 hours, then cent...

Embodiment 3

[0047] Example 3 Indoor sporangia germination test

[0048] Method: slide culture method (references: NY / T 1156.1-2006 Pesticide Indoor Bioassay Test Guidelines Fungicide Part 1: Inhibition of pathogenic fungus spore germination test concave slide method): the CB28 mixture prepared according to the method of Example 2 The bacteria solution was prepared to the corresponding concentration, and was added dropwise on the sterilized glass slides respectively, and then 10 μl of the prepared downy mildew sporangia suspension was added dropwise to the bacteria solution, with a concentration of 10 5 sporangia / ml, 4 repetitions for each treatment, with sterilized water as a control, placed in a light incubator at 25°C for 48 hours to observe the germination rate of sporangia, microscopic examination was based on 30 sporangia in the field of vision, and observed The standard is that the germ tube length is greater than 1 / 2 of the sporangia width of downy mildew bacteria, and the calculat...

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Abstract

The invention belongs to the technical field of protecting plants, and in particular relates to a bio-control combined bacterium agent CB28 admixture for preventing cucumber downy mildew. The CB28 admixture mainly comprises bacillus magaterium BS13 of which preservation number is CGMCC No.4734 and enterobacter BJN15 of which preservation number is CGMCC No.4733. Each bacterium is cultivated in LBculture liquid through vibrating at 200 rpm for 18-20 hours at 32 DEG C and centrifuged at 4000 rpm for 15 minutes, finally wet bacterium and bacterium preservation liquid are prepared as suspension bacterium liquid according to the rate of 1:40 (g / ml). Mixed preparation is formed according to the viable bacteria count rate of the bacterium of 1:1, and the concentration of the viable bacteria in the admixture is 1*109-1010 cfu / ml. The preventing effect of the CB28 admixture to the cucumber downy mildew is over 70%. The combined bacterium agent prevents the pests by spraying or spraying and root treatment at the same time.

Description

technical field [0001] The invention belongs to the technical field of plant protection, and relates to a biocontrol compound bacterial agent CB28 mixture for preventing and treating cucumber downy mildew. technical background [0002] Cucumber is one of the top ten vegetable cultivated crops in the world, and occupies an important position in my country's "vegetable basket" project. There are many diseases of its leaves, among which downy mildew is the most harmful. Therefore, it is urgent to study and control cucumber diseases in solar greenhouses. Among the cucumber leaf diseases, downy mildew is very easy to occur. Once it occurs, the area is large, the epidemic is strong, the damage is serious, and the control is difficult. It is the most important disease in the production of cucumber greenhouses. [0003] Cucumber downy mildew is a fungal disease caused by Psedoperonspora cubensis (Berkis Curt) Rostov, which occurs widely in various places. The sporangia of the fun...

Claims

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Application Information

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IPC IPC(8): A01N63/02A01P3/00C12N1/20C12R1/11C12R1/01
Inventor 郭坚华刘红霞王云鹏郑丽
Owner NANJING AGRICULTURAL UNIVERSITY
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