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Application of natural compound P44 in inhibiting activation of reproduction and growth of tumour cells

A tumor cell proliferation and compound technology, which is applied in the application field of natural compound P44 in inhibiting the reproductive growth of tumor cells, which can solve the problems of delay in the research and development of anti-tumor drugs.

Inactive Publication Date: 2013-04-24
PEKING UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, the research and development of anti-tumor drugs is relatively lagging behind, and a drug that can completely cure tumors has not yet appeared

Method used

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  • Application of natural compound P44 in inhibiting activation of reproduction and growth of tumour cells
  • Application of natural compound P44 in inhibiting activation of reproduction and growth of tumour cells
  • Application of natural compound P44 in inhibiting activation of reproduction and growth of tumour cells

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0030] Example 1, Preparation and structure identification of natural compound P44

[0031] a. Activation of the production strain Pestalotiopsis fici:

[0032] PDA medium: 200g of potatoes, 20g of glucose, 15g of agar, 1000mL of water, make a test tube slant, pick mycelium and inoculate it on the test tube slant, culture at 25°C for 7 days;

[0033] b. Solid state fermentation of production strain Pestalotiopsis fici:

[0034] Preparation of rice culture medium (add 100g rice and 100mL water into a 500mL Erlenmeyer flask, soak overnight, sterilize and cool for later use);

[0035] The prepared bacterial suspension (spore concentration of 1 × 10 6 Individuals / mL) 5mL were inoculated on the rice culture medium, and cultured at 25°C for 40 days;

[0036] c. After the fermentation is complete, add ethyl acetate into the Erlenmeyer flask for extraction three times, and evaporate the organic solvent to dryness under reduced pressure to obtain the crude extract.

[0037] d. Use ...

Embodiment 2

[0049] Example 2, High-throughput screening of natural compound P44 on tumor cell reproductive growth

[0050] Cell screening models constructed: breast cancer cells (MCF-7), lung cancer cells (A549), colon cancer cells (Caco2, SW480), African green monkey kidney fibroblasts (COS-7).

[0051] The effect of the compound on the reproductive growth of the above tumor cells was tested by the cell proliferation assay MTT method. First, the cells were inoculated into a 96-well plate. The inoculation density of each tumor cell was different, and the inoculation density of the faster-growing tumor cells was lower. Such as breast cancer cells (MCF-7). In addition, according to the treatment time of natural compound P44 on various tumor cells, the inoculation density of tumor cells should be adjusted appropriately. For example, the inoculation density of tumor cells is lower when the treatment time is longer. In order to verify the dosage of several tumor cells to the compound For each...

Embodiment 3

[0063] Embodiment 3, determination of the IC50 value of the natural compound P44 on tumor cells

[0064] The IC50 values ​​of natural compound P44 on breast cancer cells (MCF-7), lung cancer cells (A549), colon cancer cells (Caco2, SW480) and African green monkey kidney fibroblasts (COS-7) were determined.

[0065] First of all, it is necessary to determine the six treatment concentrations of the natural compound P44, which are consistent between each concentration gradient. The six concentrations are: 0.0001, 0.001, 0.01, 0.1, 1.0, 10 (uM). repeat. Controls were treated with DMSO. The inoculation density of tumor cells in the 96-well plate was adjusted according to the treatment time of the compound and the reproductive growth rate of the tumor cells (the specific inoculation density and treatment time were the same as in Example 2). Treat tumor cells with 20ul MTT per well four hours before measuring MTT value, wash off the tumor cell culture medium four hours later, add 1...

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Abstract

The invention discloses an application of natural compound P44 in inhibiting activation of reproduction and growth of tumour cells. The structure formula of the natural compound P44 is showed in formula I. The compound or the pharmaceutically acceptable salt thereof can be used for preparing the drugs for inhibiting the propagation of the tumour cells of the eucaryon or the drugs for preventing and / or treating the tumours. The in-vitro anti-tumour experiment proves that the compound P44 has better inhibiting effects on breast cancer cells (MCF-7), lung cancer cells (A549), colon cancer cells (Caco2, SW480) and vero fibrocyte (COS-7). (Formula I).

Description

technical field [0001] The invention relates to the application of a natural compound P44 in inhibiting the reproductive growth of tumor cells. Background technique [0002] Tumors are a serious threat to human health, and the morbidity and mortality rates of tumors in my country are high. At present, the research and development of anti-tumor drugs is relatively lagging behind, and a drug that can completely cure tumors has not yet appeared. Although my country has developed some anti-tumor drugs, it is still mainly imported from abroad. Therefore, the development of new anti-tumor drugs with my country's independent intellectual property rights is of great significance for improving the living standards of our people. [0003] Special biological resources formed in special habitats or extreme environments have special genes and their expression products, which are a treasure house of precious genetic resources for human beings. For example, the anti-cancer substance pac...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61K31/625A61P35/00A61P11/00
Inventor 李毅车永胜常智杰耿永涛
Owner PEKING UNIV
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