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Strain for producing citrulline and method for biologically synthesizing citrulline with same

A citrulline and strain technology, applied in the field of biological preparation of citrulline, can solve problems such as safety to be considered, residual toxic substances, complicated separation and purification operations of products, etc.

Inactive Publication Date: 2012-05-02
JIANGNAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

This method may leave toxic substances, and the safety needs to be considered, and the separation and purification of the product is complicated

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0027] The fermentation culture of embodiment 1 SK23.003 bacterial strain

[0028] Use strain SK23.003, in a medium containing 1% glucose, 1.2% arginine, 0.5% yeast extract, 0.5% peptone, 0.1% inorganic nitrogen source, and a small amount of inorganic salt, etc., to adjust the pH to 6.0 , 37°C, cultured at 190 rpm for 22 hours, refrigerated and centrifuged to obtain resting cells with arginase activity.

Embodiment 2

[0029] Example 2 Transformation reaction

[0030] Using the bacterial cells obtained in Example 1, in 100mM acetate buffer solution (HAc-NaAc, pH 6.0) containing arginine mass concentration of 50g / L and resting cell mass of 0.1-10g / L, at 37°C Conversion reaction to synthesize citrulline.

Embodiment 3

[0031] Embodiment 3 Determination of citrulline output

[0032] The reaction solution carried out in Example 2 was inactivated (TCA precipitation), diluted, and quantified by Agilent liquid chromatography for arginine and citrulline, respectively. The analysis conditions are as follows: instrument model: Agilent 1100, chromatographic column: (250*4.6) mm 5 μm ODS HYPERSIL, column temperature: 40°C.

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Abstract

The invention provides a strain for producing citrulline and a method for biologically synthesizing citrulline with the same, belonging to the food biotechnology field. The strain and the method have the following beneficial effects: the invention relates to potential new species kurthiasp.nov. (SK23.003) screened from the soil; kurthiasp.nov. has been collected in the China Center for Type Culture Collection, with collection number being CCTCC NO:M2011467; the strain is taken as the fermentation strain, arginine is taken as the inducer, glucose is taken as the carbon source and yeast cream and peptone are taken as the nitrogen sources to form a fermentation medium; citrulline is prepared through fermentation or is synthesized through transformation by adding a thallus which is produced through fermentation and contains arginase to 5-20% of arginine solution; through detection, the transformation rate can be more than 80%; citrulline produced by the method is safe and reliable, is a functional product with great market potential and is widely applied to such industries as food, cosmetics, medicines and the like; and the method can be used for efficiently producing citrulline and is suitable for large scale production.

Description

technical field [0001] The present invention relates to a microbial strain screened from soil capable of producing citrulline Kurthia sp. nov SK23.003 The method for producing citrulline by culturing and fermenting or fermenting and producing bacteria containing arginase and using the bacteria to biologically prepare citrulline is widely used in industries such as food, cosmetics, and medicine. It belongs to the field of food biotechnology. Background technique [0002] Citrulline can improve the function of the immune system, maintain the function of joint movement, balance the normal blood sugar level, contain rich antioxidants to absorb harmful free radicals, and help maintain the normal level of cholesterol. It can also relax human blood vessels, enhance male sexual function, and treat sexual dysfunction. It can also maintain healthy lung function and improve mental clarity, reduce stress and overcome depression, and help brain nerve cells have the function of storing ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20C12P13/10C12R1/01
Inventor 张涛江波王莹沐万孟缪铭
Owner JIANGNAN UNIV
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