Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Antrodia camphorata anti-cancer active substance and preparation method and application thereof

An anti-cancer active substance, Antrodia camphorata mycelium technology, applied in the direction of organic active ingredients, drug combinations, anti-tumor drugs, etc., can solve the problem of unclear bioactive components, and achieve the solution of high prices and inhibition of cancer cells. , the effect of inhibiting the growth of cancer cells

Active Publication Date: 2012-05-09
蒋丙煌
View PDF0 Cites 16 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Although there is enough evidence to show that Antrodia mycelium has the function of anti-liver cancer, its exact bioactive composition is still unclear

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Antrodia camphorata anti-cancer active substance and preparation method and application thereof
  • Antrodia camphorata anti-cancer active substance and preparation method and application thereof
  • Antrodia camphorata anti-cancer active substance and preparation method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0038] The preparation of embodiment 1 Antrodia camphorata fermented liquid

[0039] 1.1 Source of Antrodia cintrodia

[0040] The Antrodia camphorata used in this embodiment is obtained from the Antrodia camphorata BCRC35716 (commercially available) deposited in the Food Industry Development Institute (Hsinchu, Taiwan) Biological Resources Preservation and Research Center, but the active substances of Antrodia camphorata described in the present invention are not limited to derived from this strain.

[0041] 1.2 Antrodia camphorata liquid fermentation culture:

[0042] Inoculate the mycelia of Antrodia camphorata into 5 tons of fermentation broth (pH 5.0) containing 2% glucose and 2% malt extract in volume, place it at 22°C at a speed of 50 revolutions per minute (50rpm), and Minute 0.5 times unit liquid volume (0.5vvm) aeration culture, culture for four weeks.

[0043] 1.3 Treatment of Antrodia camphorata liquid fermentation broth:

[0044] After the fermentation and cul...

Embodiment 2

[0045] The preparation of embodiment 2 Antrodia camphorata active substance

[0046] Redissolve the ethanol crude extract of Antrodia camphorata (857g) in 2L of water, and extract with an equal volume of ethyl acetate; then extract the aqueous layer with water-saturated n-butanol 3 times. The crude extract, ethyl acetate extract, n-butanol extract, and water layer were used to test their effect on inhibiting the proliferation of HepG2 liver cancer cell line.

[0047] The above-mentioned extract with the most antitumor activity was further purified by silica gel chromatography (silica gelchromatography, 230-400 mesh, 750-75mm), and was washed with n-hexane / ethyl acetate gradient (hexane / ethyl acetate). gradient elution, the ratio of n-hexane / ethyl acetate from 100:0 to 0:100), and finally 100% methanol (Methanol) to wash out the last residue. Collect each eluate (700 mL), and use thin-layer chromatography (thin-layer chromatography, TLC Silica gel 60F254, Merck Co.) with ethy...

Embodiment 3

[0054] Example 3 Analysis of the Inhibitory Ability of Active Substances of Antrodia Antrodia to Inhibit Liver Cancer Cell Proliferation

[0055] 3.1 Materials

[0056] HepG2 cells were purchased from American Type Culture Collection, ATCC, Rockville, Maryland, USA; HepG2 cells were cultured in WME (Williams medium E) medium containing 10 mM 4-(2- Hydroxyethyl)-1-piperazineethanesulfonic acid [4-(2-hydroxyethyl)-1-piperazineethanesulfonic acid, Hepes], 5 μg / mL insulin, 2 μg / mL glucagon, 0.05 μg / mL hydrocortisone and 5% fetal bovine serum (Gibco Life Technologies, Grand Island, NY, USA). Colorectal cancer cells CT26 and prostate cancer cells LNCaP were purchased from BCRC 60443 and BCRC 60088 from the Bioresource Conservation and Research Center of the Food Industry Development Institute.

[0057] 3.2 Method

[0058]In a 96-well flat-bottomed cell culture dish, 2.5×104 HepG2 liver cancer cells per well were planted, cultured in WME based on 37°C, 5% CO2 environment for 4 ho...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

PropertyMeasurementUnit
Half inhibitory concentrationaaaaaaaaaa
Login to View More

Abstract

The invention discloses an antrodia camphorata anti-cancer active substance and a preparation method and application thereof. The antrodia camphorata anti-cancer active substance is prepared by the following steps of: (1) fermenting and culturing an antrodia camphorate mycelium with a liquid; (2) extracting the obtained mycelium with ethanol to obtain an ethanol extract; (3) concentrating the obtained ethanol extract, dissolving into the water, and adding an equal volume of acetic ether for extracting to obtain an acetic ether extract; (4) purifying the obtained acetic ether extract by performing colloid pipe column chromatography, and extracting an eluent segment g from a n-hexane / acetic ether gradient eluent as a mobile phase; and (5) purifying the obtained eluent segment g by performing colloid pipe column chromatography, and extracting a specific eluent segment from the n-hexane / acetic ether gradient eluent to obtain the antrodia camphorata anti-cancer active substance. The antrodia camphorata anti-cancer active substance provided by the invention is purified to a single component, and the effect of resisting cancer cells can be realized under the condition of very low dosage. The single component can be synthesized by using a chemical synthesis method, so that an anti-cancer effect is achieved.

Description

technical field [0001] The invention relates to an anticancer active substance of Antrodia camphorata, a preparation method and its application, in particular to an active substance of Antrodia camphorata that can inhibit the proliferation of liver cancer cells to achieve the effect of anti-liver cancer. Background technique [0002] Antrodia cinnamomea is a microorganism parasitic in the hollow trunk of Cinnamomum kanchirai Hayata, Lauraceae, native to Taiwan. Antrodia Cinnamomea has multiple health-promoting effects, including antioxidant, anti-inflammatory, and antitumor activities (Chang and Chou, 2004; Mau, Huang, Huang, and Chen, 2004; Song and Yen, 2003; Hseu, Chang, Hseu, Lee, Yech, and Chen, 2002; Shen, Chou, Wang, Chen, Chou, and Lu, 2004; Ao et al., 2009). The fungus has traditionally been used to treat liver diseases, but its antitumor activity, especially against liver cancer, has attracted extensive attention. [0003] Several compounds contained in the fruit...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
IPC IPC(8): C12P17/04C12P1/02A61K31/365A61P35/00
Inventor 蒋丙煌林育蔚
Owner 蒋丙煌
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com