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Electrostatic spinning method of low molecular weight collagen peptide and chitosan oligosacchaides biological membrane

A technology of chitooligosaccharides and low molecular weight, applied in the field of preparation of composite nanofiber membranes, can solve the problems of insignificant antibacterial effect, poor hydrophilicity of chitosan, slow degradation rate, etc., and achieve adhesion and wound healing The effect of ability improvement

Inactive Publication Date: 2012-05-30
TONGJI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Due to the poor hydrophilicity, slow degradation rate and insignificant antibacterial effect of pure chitosan, it needs to be modified or compounded with other materials to improve it.

Method used

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  • Electrostatic spinning method of low molecular weight collagen peptide and chitosan oligosacchaides biological membrane
  • Electrostatic spinning method of low molecular weight collagen peptide and chitosan oligosacchaides biological membrane
  • Electrostatic spinning method of low molecular weight collagen peptide and chitosan oligosacchaides biological membrane

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0041] Preparation of polypeptide / chitooligosaccharide / PVA biofilm

[0042] One of the spinning solutions was prepared: 0.2 g of low-molecular-weight collagen peptide was dissolved in 6 ml of double-distilled water, and placed at room temperature until completely dissolved.

[0043] The second configuration of the spinning solution: 0.3 g of chitooligosaccharides was dissolved in 5 ml of formic acid solvent, and placed at 4° C. until completely dissolved.

[0044] The third preparation of the spinning solution: dissolve 0.6 g of PVA in 10 ml of double distilled water, shake and mix well, and let stand at room temperature until completely dissolved.

[0045] The above three spinning solutions were mixed, put into a magnetic stirring rotor, stirred and mixed for about 3 hours, and refrigerated at 4°C for later use. The mixed spinning solution needs to be stirred in a mixer for about 1 hour to make it evenly mixed before use, and ultrasonically degassed for 15 minutes before usi...

Embodiment 2

[0049] A biofilm electrospinning method of low molecular weight collagen peptides and chitooligosaccharides, the method comprising the following steps:

[0050] (1) Preparation of spinning solution:

[0051] dissolving chitooligosaccharides with deacetylation degree>90% and viscosity<100cps in formic acid to prepare a chitooligosaccharide formic acid solution with a mass concentration of 3%;

[0052] Dissolving low-molecular-weight collagen peptides in double-distilled water to prepare an aqueous solution of collagen peptides with a mass concentration of 8%;

[0053] Dissolving polyvinyl alcohol in double distilled water to prepare an aqueous polyvinyl alcohol solution with a mass concentration of 5%;

[0054] (2) Chitooligosaccharide formic acid solution and collagen peptide aqueous solution are mixed in a weight ratio of 1: 3, then add polyvinyl alcohol aqueous solution in the mixture obtained, and the weight ratio of the mixed solution and polyvinyl alcohol aqueous solutio...

Embodiment 3

[0056] A biofilm electrospinning method of low molecular weight collagen peptides and chitooligosaccharides, the method comprising the following steps:

[0057] (1) Preparation of spinning solution:

[0058] dissolving chitooligosaccharides with deacetylation degree>90% and viscosity<100cps in formic acid to prepare a chitooligosaccharide formic acid solution with a mass concentration of 5%;

[0059] Dissolving low-molecular-weight collagen peptides in double-distilled water to prepare an aqueous solution of collagen peptides with a mass concentration of 16%;

[0060] Dissolving polyvinyl alcohol in double distilled water to prepare an aqueous polyvinyl alcohol solution with a mass concentration of 8%;

[0061] (2) the chitooligosaccharide formic acid solution and the collagen peptide aqueous solution are mixed in a weight ratio of 1: 2, and then an aqueous polyvinyl alcohol solution is added in the resulting mixture, and the weight ratio of the mixed solution and the aqueous...

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Abstract

The invention relates to an electrostatic spinning method of a low molecular weight collagen peptide and chitosan oligosacchaides biological membrane, wherein appropriate hydrophile carrier high polymer polyvinyl alcohol (PVA) is added to low molecular weight collagen peptide / chitosan oligosacchaides solution to prepare collagen peptide / chitosan oligosacchaides / PVA blening spinning solution, and the blending solution with uniformly distributed charges is prepared through utilizing the properties of different solvents. Compared with the prior art, a biological membrane has the smooth fiber surface and more uniform fiber thickness, the fiber diameters are distributed between 50-100nm, the biocompatibility, adhesivity, wound healing capability and other performance can be improved, and the method can be better and more widely applied in the pharmaceuticals industry.

Description

technical field [0001] The invention relates to the field of preparation of composite nanofiber membranes, in particular to a biological membrane electrostatic spinning method of low molecular weight collagen peptides and chitooligosaccharides. Background technique [0002] With the advancement of modern science and technology, especially the development of materials science and pharmacy, the application of textile technology in biomaterials continues to develop. Especially in recent years, due to the upsurge of nanotechnology, there are more and more reports on the preparation of functional nanofibrous membranes by high-voltage electrospinning technology. Researchers have attempted to prepare various polymeric materials (including natural and synthetic polymers, proteins, and even small molecules such as lecithin) into nanofibrous membranes through electrospinning to exert the functional effects of materials at the nanoscale . [0003] Collagen peptide is the degradation ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): D01D1/02D01D5/00D01F8/18D01F8/02D01F8/10
Inventor 李平王艳张群
Owner TONGJI UNIV
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