Rapid propagation method for Leersia hexandra Swartz
A node and naphthalene acetic acid technology, applied in the field of chromium-contaminated phytoremediation, can solve the problems of not being able to meet large-scale engineering repairs, difficulty in distinguishing individual seedlings, and low seed germination rate, achieving no seasonal influence, shortening the growth cycle, The effect of quick process
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[0012] Choose 50 vigorously growing Lishihe plants with multiple nodes, at the second node, cut the Lishihe stem tip, and keep 1.0-1.5cm stem section at the end of the node (such as figure 1 shown). The blade destroys the epidermis of the nodes along the periphery, exposing the cambium; the terminal nodes of Lishihe are immersed in 5 mg / L naphthalene acetic acid solution and placed in a dark artificial climate box. After 6 hours, the naphthalene acetic acid solution at the end of Lishihe is cleaned with distilled water Clean, 10 plants are packaged in a group of improved hoagland nutrient solution for cultivation, and the cultivation environment is set as follows: light intensity 2000LX, relative humidity 75%, daytime 15h / d, temperature 25±1℃, nighttime 9h / d, temperature 20±1 ℃. Such as figure 2 As shown, after cultivating for 7 days, the nodes will take root and grow seedlings. The rooted Lishihe is transplanted into the soil and cultivated in a natural environment, and w...
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