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Reagent used for detecting urinary iodine by biochemistry analyzer and urinary iodine detecting method

A biochemical analyzer and quantitative detection technology, applied in biological testing, material inspection products, etc., can solve the problems of poor objectivity of experimental results and long detection time, and achieve less reagent consumption, short detection time, and small human error. Effect

Active Publication Date: 2012-06-13
WUHAN ZHONGSHENG BIOCHEM TECHN
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Problems solved by technology

This method is for hospitals (especially large hospitals) to carry out urine iodine detection items. Because the operation steps are all manual operations, the detection time is long, and the reaction end point needs to be judged by naked eyes. The objectivity of the experimental results is not strong, so most hospitals still use it. unwilling to accept

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  • Reagent used for detecting urinary iodine by biochemistry analyzer and urinary iodine detecting method
  • Reagent used for detecting urinary iodine by biochemistry analyzer and urinary iodine detecting method

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[0048] The present invention will be further described below in conjunction with the examples, but the examples should not be construed as limiting the present invention.

[0049] 1. Reagent composition,

[0050] (1) Digestion solution: ammonium persulfate solution {c[(NH 4 )2 S 2 o 8 ]=1.3mol / L};

[0051] (2) Reagent R 1 : Arsenous acid solution [c(H 3 AsO 3 )=0.037mol / L];

[0052] (3) Reagent R 2 : Ammonium cerium sulfate solution [c(Ce 4+ )=0.0076mol / L];

[0053] (4) Series of iodine standard solutions: Potassium iodate solution, the iodine content is respectively: 0, 50, 100, 200, 300, 400 μg / L.

[0054] 2. Digestion,

[0055] Accurately draw a series of iodine standard solution and 300 μl of urine sample into test tubes, add 600 μl of digestion solution each, and place in an electric heating oven for digestion at 100°C for 1 hour; during digestion, the vent on the top of the electric oven should be open and the blower should not be turned on; The digested samp...

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Abstract

The invention discloses a reagent used for detecting urinary iodine by a biochemistry analyzer and a urinary iodine detecting method. The reagent mainly comprises the following components: digestion solution-ammonium persulfate solution, reagent R1-arsenious acid or arsenic trioxide solution, reagent R2-ceric ammonium sulfate or cerous sulfate solution, and iodine standard solution-potassium iodide (sodium iodide) or potassium iodate (sodium iodate) solution. The detecting method mainly includes the following steps: selecting the detecting reagent, digesting urine sample, setting parameters of the biochemistry analyzer, and determining through the biochemistry analyzer. The detecting method has high automation degree, small human error, little reagent dosage and short detecting time. By adopting the biochemistry analyzer to analyze the urinary iodine, the urinary iodine detecting method expands the detecting items of the instrument, is particularly suitable for the medical organizations equipped with the biochemistry analyzers, and meets the requirements of clinical detection on quick issuing of result and detecting automation.

Description

technical field [0001] The invention relates to a medical detection reagent and a detection method, in particular to a reagent for quantitative detection of urinary iodine by a biochemical analyzer and a detection method of urinary iodine. Background technique [0002] Urinary iodine level is an important index to evaluate the iodine intake status of the population, which has been widely used at home and abroad. Currently, iodine deficiency and iodine excess have become public health issues of equal concern. Urinary iodine detection has become an important basis for guiding the scientific iodine supplementation of the population. [0003] Urinary iodine detection is a complex ultra-trace analysis technique. At present, there are three methods that can accurately determine urinary iodine and are adopted at home and abroad as follows: [0004] (1). Ion chromatography mass spectrometry (ICP-MS), hereinafter referred to as method (1). This method uses a plasma chromatograph ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/50G01N33/52
Inventor 刘列钧
Owner WUHAN ZHONGSHENG BIOCHEM TECHN
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