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Method for refining camellia seed crude oil beneficial to individuals with hypertension, hyperglycemia, hyperlipidemia

A technology of Camellia oleifera seed crude oil and people, which is applied in the direction of fat oil/fat refining and fat production, can solve the problems affecting the quality of finished oil, the effect of tea seed oil, deepening the color of tea oil, etc., to achieve cost reduction and sensitivity reduction , The effect of easy operating conditions

Active Publication Date: 2012-06-20
FUJIAN CHUNHUI BIOLOGICAL ENG
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The content of unsaturated fatty acids in camellia oil is relatively high, accounting for about 80%. Chemical degumming requires high temperature, which is easy to oxidize the unsaturated fatty acids contained in tea oil, which affects some of the original effects of tea seed oil.
Pigment substances formed due to high temperature will deepen the color of camellia oil, and these substances are difficult to remove in the subsequent decolorization process, which affects the quality of finished oil

Method used

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  • Method for refining camellia seed crude oil beneficial to individuals with hypertension, hyperglycemia, hyperlipidemia

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0028] In the present embodiment, refining camellia seed crude oil with immobilized phospholipase A1 is realized through the following steps:

[0029] a. Add 5 mg of phospholipase A1 to 50 ml of HAc-NaAc or citric acid buffer solution with a pH value of 7 to obtain an enzyme solution. The concentration of phospholipase A1 in the enzyme solution is 1 mg / ml; the pretreated The macroporous resin and the enzyme solution are mixed at a volume ratio of 6:1 to obtain a mixed solution; the container containing the mixed solution is placed on a shaker and reacted at a speed of 150 rpm for 2 hours, and then added with a concentration of 0.03% The glutaraldehyde was cross-linked for 1 hour, and the cross-linking temperature was controlled at 3°C. The filter cake was collected by vacuum filtration, and the obtained was immobilized phospholipase A1. After dispersing the filter cake, put it in the refrigerator, and let it stand and dry at 0°C;

[0030] B, take the camellia seed crude oil an...

Embodiment 2

[0035] In the present embodiment, refining camellia seed crude oil with immobilized phospholipase A1 is realized through the following steps:

[0036]a, 20mg of phospholipase A1 is added to 100ml, and the pH value is 7 in HAc-NaAc or citric acid buffer solution to obtain enzyme liquid, the concentration of phospholipase A1 in the enzyme liquid is 1mg / ml; pretreated The macroporous resin and the enzyme solution are mixed at a volume ratio of 7:1 to obtain a mixed solution; the container containing the mixed solution is placed on a shaker and reacted at a speed of 170 rpm for 3 hours, and then added with a concentration of 0.1% The glutaraldehyde was cross-linked for 3 hours, and the cross-linking temperature was controlled at 3°C. The filter cake was collected by vacuum filtration, and the obtained was immobilized phospholipase A1. After the filter cake was dispersed, put it in the refrigerator, and let it stand and dry at 3°C;

[0037] B, take the camellia seed crude oil and r...

Embodiment 3

[0041] In the present embodiment, refining camellia seed crude oil with immobilized phospholipase A1 is realized through the following steps:

[0042] a, 50mg of phospholipase A1 is added to 500ml, and the pH value is 7 in HAc-NaAc or citric acid buffer solution to obtain enzyme liquid, the concentration of phospholipase A1 in the enzyme liquid is 3mg / ml; pretreated The macroporous resin and the enzyme solution are mixed at a volume ratio of 9:1 to obtain a mixed solution; the container containing the mixed solution is placed on a shaker and reacted at a speed of 180 rpm for 4 hours, and then added with a concentration of 0.24% The glutaraldehyde was cross-linked for 6 hours, and the cross-linking temperature was controlled at 15°C. The filter cake was collected by vacuum filtration, and the obtained was immobilized phospholipase A1. After the filter cake was dispersed, put it in the refrigerator, and let it stand and dry at 5°C;

[0043] B, take the camellia seed crude oil an...

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Abstract

The invention relates to a method for refining camellia seed crude oil, in particular to a method for refining the camellia seed crude oil by using immobilized phospholipase A1. The technical scheme of refining the camellia seed crude oil by using the immobilized phospholipase A1provided by the invention is realized by the following steps of: a, immobilizing phospholipase; b, carrying out pre-treatment on the camellia seed crude oil; c, adding the immobilized phospholipase into the camellia seed crude oil; and d, agitating and then centrifuging, namely realizing that the camellia seed crude oil is refined by using the immobilized phospholipase. The method for refining the camellia seed crude oil provided by the invention has the beneficial effects that unsaturated fatty acid is lost for 1-3% and is reduced by 15-20% compared with the loss of the unsaturated fatty acid in chemically-refined camellia seed oil; the immobilized phospholipase A1 is degummed to reduce phosphorus content in the oil to be lower than 5 mg / kg; the heat resistance of the immobilized phospholipase A1 is improved; the immobilized phospholipase A1 can keep a higher activity in a wider pH value range and the sensitivity of the immobilized phospholipase A1 to a reaction environment is reduced, so that the operation conditions are easier to control and implement.

Description

technical field [0001] The invention relates to a method for refining camellia seed crude oil, in particular to a method for refining camellia seed crude oil by using immobilized phospholipase A1. Background technique [0002] Camellia Oleifera Ahel is a unique woody oil plant in my country. It is mainly distributed in the mountains and hills of 14 provinces (regions) in the south of my country. At present, there are about 4 million hectares of Camellia oleifera forests in the country. The annual output of Camellia oleifera seeds exceeds 600,000 tons. Camellia oil extracted from Camellia oleifera seeds has high nutritional value and health care function, and its fatty acid composition is similar to that of olive oil, so it is called "Oriental olive oil". Tea oil contains tea polyphenols and camellia glycosides, rich in VE, VD, VK, carotene and squalene. Long-term consumption can enhance the elasticity and toughness of blood vessels, delay atherosclerosis, increase gastroint...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C11B3/00C11B3/04
Inventor 刘春雷李丹周伯川彭彪蔡春辉
Owner FUJIAN CHUNHUI BIOLOGICAL ENG
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