Isoleucine fermenting process

A technology of isoleucine and fermentation process, applied in the field of isoleucine fermentation process, can solve the problems of continuous, low level of automation, low level, high yield but not good harvest, etc., to reduce fermentation cost and extraction cost, and reduce metabolism Formation of by-products, favorable effect on product purity

Inactive Publication Date: 2012-06-20
KAIYUAN TIANMU BIOLOGICAL TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0007] (4) As far as the amino acid industry is concerned, the pollution control is not enough, and there is still a certain distance from the national requirements, and the environmental protection task is quite heavy and arduous
[0011] (2) Less investment in research and development, fewer varieties, poor adaptability and anti-risk ability, which affect the independent innovation of the enterprise and the improvement of the technical level, resulting in insufficient stamina for the development of the enterprise;
[0012] (3) The environmental protection management is not th

Method used

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  • Isoleucine fermenting process
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  • Isoleucine fermenting process

Examples

Experimental program
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Example Embodiment

[0030] Example 1

[0031] At 50m 3 Fermentation tank and 7 m 3 In the practice of seed tank production, the steps are as follows:

[0032] (1) Culture strain

[0033] Select high-yielding strains that grow on plates containing 200g / L glucose and 35g / L isoleucine, expand the culture in the strain room to produce first-level seeds, and then connect them to the second-level seed tank to continue the expansion. The secondary seed medium formula in the secondary seed tank is: glucose: 2.2g / L, corn steep liquor: 20g / L, dipotassium hydrogen phosphate: 3g / L, ammonium sulfate: 25 g / L, magnesium sulfate: 0.15 / g / L, V H :3.0μg / L, molasses: 25g / L, thiamine: 500μg / L, V B1 :0.2g / L, the bacterial species index obtained by the secondary seed liquid after actual elimination, cooling and cultivation in the secondary seed tank is: △OD=1.05, Gram staining is positive;

[0034] (2) Fermentation

[0035] Connect the secondary seed liquid to the fermentor containing the fermentation medium. The amount of th...

Example Embodiment

[0048] Example 2

[0049] The difference between Example 2 and Example 1 is that the secondary seed culture medium formula and the fermentation medium formula are different, and the process steps are the same as those of Example 1, so they are omitted. The secondary seed culture medium formula is: glucose: 3.0g / L, corn steep liquor: 25g / L, dipotassium hydrogen phosphate: 3g / L, ammonium sulfate: 30g / L, magnesium sulfate: 0.15 / g / L, V H :4.0μg / L, molasses: 50g / L, thiamine: 600μg / L, V B1 :0.3g / L, the bacterial species index obtained by the secondary seed liquid after actual elimination, cooling and cultivation in the secondary seed tank is: △OD=1.16, Gram staining is positive;

[0050] The formula of fermentation medium is: glucose: 160g / L, ammonium sulfate: 50g / L, corn steep liquor: 20g / L, magnesium sulfate: 0.2g / L, potassium dihydrogen phosphate: 3.0g / L, V B1 :0.2g / L, V H : 4.0μg / L, molasses: 30g / L, thiamine: 600μg / L, the secondary seed liquid and fermentation medium are continuously ...

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Abstract

The invention relates to an isoleucine fermenting process. The key points are that: the isoleucine fermenting process comprises the following steps of: (1) culturing strains; (2) fermenting; (3) separating fermenting solution; (4) carrying out ion exchange in clear liquid of the fermenting solution; and (5) evaporating and crystallizing. According to the process provided by the invention, better fermenting index is generated; the yield of a metabolic product of isoleucine is increased, and the acid production rate is averagely increased by 0.48 percentage point in comparison with that before innovation; the conversion rate of the metabolic product of isoleucine is increased, and the sugar-acid conversion rate is increased by 3.2 percentage point; the generated strains can realize rapid growth and metabolism, the fermenting cycle is shortened by 4-6 h, the turnover rate of equipment is increased, and the yield is increased correspondingly; and metabolic by-products are reduced, the purity of metabolic products in corresponding fermenting solution is improved correspondingly, the process is favorable for extracting operation of products and purity of products, and the fermenting and extracting costs are reduced at the same time.

Description

technical field [0001] The invention relates to the technical field of amino acid production, in particular to a fermentation process for isoleucine in branched-chain amino acids. Background technique [0002] my country's amino acid production industry is a growing industry, mainly glutamic acid and lysine. Branched-chain amino acids—such as isoleucine, valine and other industries developed relatively late, with a low start and backward technology, and there has been an imbalance in the development of the amino acid industry, and the supply of some amino acids exceeds the demand. The production cost of branched-chain amino acids cannot be reduced, and the quality of the products is poor, and the price cannot go up. Most of the branched-chain amino acids produced cannot be used in the production of feed, and the quality cannot reach the pharmaceutical grade. Many domestic amino acid factories are in urgent need of high-quality amino acids. High-quality, low-cost production ...

Claims

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Application Information

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IPC IPC(8): C12P13/06
Inventor 慕忠杰张志岩
Owner KAIYUAN TIANMU BIOLOGICAL TECH
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