Method for rapidly detecting poisoned substances by adopting urine

Abstract

The invention discloses a method for rapidly detecting poisoned substances by adopting urine, which comprises the following steps of: taking the urine to be detected and adding pH9 buffer solution; pouring mixed solution into a chromatographic column filled with diatomaceous earth; eluting by using methylene chloride; collecting eluent; furthest volatilizing in water bath with the temperature of 50 DEG C; and then detecting by utilizing a conventional compound detecting instrument to obtain the poisoned substances. The invention establishes the diatomaceous earth rapid detection method which is suitable for 10 toxic substances (basically covering all the toxic substances) such as a barbital sleeping medicament, a benzodiazepine sleeping medicament, a phenothiazine sleeping medicament, common drugs, alkaloid, indandione chronic rodenticides, hydroxy coumarin chronic rodenticides, tetramine acute rodenticides, organophosphorus pesticides, carbamate pesticides and the like in the urine. In the method, only one sample is required, the extraction is completed in five minutes to carry out detection, and impurities are completely removed. The poisoned substances can be rapidly detected. The detecting time is shortened. The detection operation is greatly simplified.

Description

technical field [0001] The invention belongs to the field of compound detection, and in particular relates to a method for rapidly detecting the poison in urine by using urine. Background technique [0002] The analysis and testing of drugs, poisons and narcotics in urine is divided into two steps: extraction and detection. Due to the advancement of science and technology, the instrumental analysis and detection technology has made great progress, but the extraction technology is still in the development stage. The establishment of a simple, convenient and fast extraction system with high extraction rate and thorough removal of impurities is suitable for the analysis and testing of various drugs, poisons and drugs. method is particularly important. [0003] Before the 1990s in my country, the traditional and classic liquid-liquid extraction method was mainly used. This method has the disadvantages of cumbersome operation, solvent consumption, low drug extraction rate, poor ...

AI Technical Summary

Problems solved by technology

[0003] Before the 1990s in my country, the traditional and classic liquid-liquid extraction method was mainly used. This method has the disadvantages of cumbersome operation, waste of solvent, low drug extraction rate, poor reproducibility, incomplete removal of impurities, and easy emulsification.

In the early 1990s, my country began to study the extraction method of lipophilic solid-phase materials (mainly GDX). This technology avoided many shortcomings of liquid-liquid extraction, and the extraction rate was significantly improved, but there were still cumbersome operations and time-consuming (urine test materials Extraction generally takes 120 minutes) and other issues

At present, the extraction methods studied are generally only for a single or single class of drugs. For example, for these 50 or 60 kinds of poisons, multiple methods are required to purify them separately. No one method can be used for all, and multiple samples need to be prepared for detection. In order to cover all the poisons, the workload is very large, and it takes a long time to detect that kind of poisons at the same time, so rapid detection cannot be achieved.

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