Method using liquid phase chip to detect mononuclear cell proliferation listeria

Abstract

A method using a liquid phase chip to detect mononuclear cell proliferation listeria belongs to the field of immunity technology and food sanitation detection technology. The invention aims to provide the method using the liquid phase chip to detect mononuclear cell proliferation listeria, so as to realize rapid detection of mononuclear cell proliferation listeria. In the invention, the liquid phase chip is prepared and then applied to detect mononuclear cell proliferation listeria. Compared with other detection methods, the method using the liquid phase chip to detect mononuclear cell proliferation listeria has advantage of high two-way throughput of detection sample amount and detection items (a large amount of samples can be detected in items as many as 96), and has the characteristics of good flexibility, high sensitivity, good SNR (signal to noise ratio), convenience for operation, wide standard curve range, wide applicable range and the like. The liquid phase chip technology, as an advanced service platform of bioinformatics, has wide applicable prospect in fields like clinical diagnosis, veterinarian communicable disease diagnosis, food microorganism detection and so on.

Description

technical field [0001] The invention belongs to the technical field of immune technology and food sanitation detection. Background technique [0002] There are many detection methods for the detection of foodborne pathogen Listeria monocytogenes, such as traditional culture detection methods, immunological detection methods, PCR technology and nucleic acid probes. Although these methods can provide relatively sensitive and specific qualitative or quantitative methods, they can only detect one pathogen at a time, which becomes cumbersome, time-consuming and expensive when used for the detection of multiple pathogens. Contents of the invention [0003] The purpose of the present invention is to provide a method for detecting Listeria monocytogenes by using liquid phase chip technology, so as to realize the rapid detection of Listeria monocytogenes. [0004] The invention consists of microspheres, capture antibodies, detection antibodies, biotin-labeled anti-antibodies, stre...

AI Technical Summary

Problems solved by technology

Although these methods can provide relatively sensitive and specific qualitative or quantitative methods, they can only detect one pathogen at a time, which becomes cumbersome, time-consuming and expensive when used for the detection of multiple pathogens

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