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Culture method for increasing number and activity of alfalfa root nodules

A method of cultivating alfalfa root nodules, which is applied in the field of agriculture, can solve the problems affecting the yield and quality of alfalfa, the number of root nodules, and the reduction of root nodule advantages, etc., and achieve the effects of improving alfalfa quality, increasing activity, and increasing alfalfa yield

Inactive Publication Date: 2014-04-16
NORTHWEST A & F UNIV
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Problems solved by technology

At present, the conventional alfalfa root nodule culture method is soil cultivation, which can cultivate root nodules, but the number of root nodules cultivated is small and the activity is low, thereby reducing the advantage of root nodules in the growth of alfalfa, and subsequently affecting the growth of alfalfa. yield and quality

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  • Culture method for increasing number and activity of alfalfa root nodules
  • Culture method for increasing number and activity of alfalfa root nodules

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Embodiment Construction

[0008] In order to further illustrate technical scheme of the present invention, adopt following concrete scheme to cultivate alfalfa root nodule, it may further comprise the steps:

[0009] After the alfalfa seeds were sterilized (soaked in 5% sodium hypochlorite for 5 minutes and soaked in 70% alcohol for 10 seconds), they were placed in a petri dish covered with filter paper (100 seeds per petri dish), and placed in a light incubator (light 25°C, 12h / dark (25°C, 12h) for germination, pay attention to replenish water during the period. After 3-4 days of germination, transplant it into a cultivation pot filled with quartz sand. Carry out a rhizobia inoculation while transplanting. Thereafter, watering was carried out with a total nitrogen nutrient solution (Table 1).

[0010] Table 1 Nutrient Solution Formula

[0011]

[0012] Carry out rhizobia inoculation again when seedling grows to about 10cm. Then, the nitrogen concentration of the nutrient solution was lowered to...

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Abstract

The invention discloses a culture method for increasing the number and activity of alfalfa root nodules. The culture method includes the following steps: after being disinfected, alfalfa seeds are placed into a culture dish with a piece of filter paper, and are germinated for 3 to 4 days in an illuminating incubator, the seedlings are then transplanted into a culture bowl filled up with quartz sand, rhizobia are inoculated during transplantation, nutrient solution is poured in the culture bowl, rhizobia are inoculated once again when the seedlings are grown to about 10cm, the nitrogen concentration of the nutrient solution is then decreased to less than 5mM, the nutrient solution is poured for one day, and water is poured for another day. The culture method can greatly increase the activity of alfalfa root nodules and the number of rhizobia and sufficiently exert the effect of root nodules in the growth of alfalfa, thus increasing the yield of alfalfa and improving the quality of alfalfa.

Description

technical field [0001] The invention belongs to the field of agriculture, and in particular relates to a cultivation method for increasing the number and activity of alfalfa root nodules. Background technique [0002] Alfalfa (Medicago sativa L.), also known as alfalfa and alfalfa, is known as the "king of herbage". It is the earliest cultivated, most widely distributed, and also the best and most important high-quality leguminous forage in the world. Alfalfa has been cultivated in my country for more than 2,000 years. It is planted all over the country, but it is mainly distributed in Northwest and North China. The output of alfalfa hay ranks first among many kinds of leguminous forages, and it has complete nutritional components, rich in protein, trace elements and multivitamins, and has the potential to be developed into health care products, food additives and raw materials for the production of biopharmaceuticals. Due to its well-developed root system and biological ni...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01C1/02C05G3/00A01G31/02
CPCY02P60/21
Inventor 杨培志王卫栋张攀呼天明苗彦军张志强
Owner NORTHWEST A & F UNIV