Calcium-complex starch-based microporous haemostatic material, and preparation method and application thereof

A hemostatic material, starch-based technology, applied in medical science, bandages, absorbent pads, etc., can solve the problems of difficult in vivo degradation, poor biocompatibility, and high production cost, and achieve good biocompatibility, low cost, and easy preparation Effect

Active Publication Date: 2014-08-13
苏州佰济生物科技有限公司
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0011] The purpose of the present invention is to overcome the shortcomings of current hemostatic materials such as poor biocompatibility, difficulty in in vivo degradation, and high production cost, and provide a calcium complexed starch-based microporous hemostatic material and its preparation method and application

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  • Calcium-complex starch-based microporous haemostatic material, and preparation method and application thereof
  • Calcium-complex starch-based microporous haemostatic material, and preparation method and application thereof
  • Calcium-complex starch-based microporous haemostatic material, and preparation method and application thereof

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preparation example Construction

[0062] Preparation method of starch-based microporous hemostatic material

[0063] The preparation method of the starch-based microporous hemostatic material of the present invention comprises the following steps:

[0064] (1) Preparation of microporous starch

[0065] Add starch and enzymes to phosphate buffer, stir at 45-80°C and pH 5-7 to carry out enzymolysis reaction, centrifuge after 4-15 hours, and dry to obtain microporous starch; wherein,

[0066] The mass ratio of the starch and the enzyme is 1: (0.01-0.2);

[0067] The mass volume ratio of the total amount of the starch and enzyme to the phosphate buffer is 100g: (250-1000) ml;

[0068] (2) Carboxymethylation of microporous starch

[0069] Dissolve monochloroacetic acid in ethanol, add sodium hydroxide solution to pH 6.5-7, mix it into the microporous starch prepared in the step (1) of dissolving with ethanol, stir well, and put it under the condition of 50-80°C Continue to stir and keep warm to react for 4-8 ho...

Embodiment 1

[0110] First, weigh 100g of tapioca starch and 1g of glucoamylase, add them into 250ml of phosphate buffer solution with pH=5, stir evenly, carry out hydrolysis reaction at 45°C for 4 hours, centrifuge at 4000r / min, and Vacuum dried for 24 hours to obtain microporous starch.

[0111] Dissolve 8g of monochloroacetic acid in ethanol, adjust it to neutral with 0.3mol / L sodium hydroxide solution, mix it into the above-prepared microporous starch dissolved in 80g of ethanol, stir well and move it into a constant temperature tank. Under the condition of 50° C., the reaction was carried out with continuous stirring and heat preservation at 45 r / min. After 4 hours, the reaction was completed, and acetic acid was added to neutralize the pH to 6.5, filtered, washed 3 times with ethanol, and dried for 24 hours to obtain carboxymethyl microporous starch.

[0112] Finally, add the carboxymethylated microporous starch obtained in the above reaction into a saturated calcium chloride solutio...

Embodiment 2～4

[0114] Examples 2-4 Repeat the experimental steps of Example 1, the difference lies in some experimental conditions, as shown in Table 1-3.

[0115] Adopt scanning electron microscope (SEM) to observe respectively the pure microporous starch that embodiment 1 prepares (see appendix figure 2 (a) and (b)) and the microscopic appearance of the calcium complex type microporous starch prepared by embodiment 2, embodiment 4 (see attached figure 2 (c) and 2(d)). It can be seen from the figure that the diameter of the prepared calcium-complexed microporous starch particles is about 10-20 μm, and the surface is covered with small pores with a diameter of about 1-5 μm, and the pores are embedded from the surface to the center to form a cavity.

[0116] The average pore size of the calcium-complexed microporous starch prepared in Examples 1-4 is shown in Table 4. It can be seen from Table 4 that with the prolongation of the enzymatic hydrolysis reaction time, the average pore size of...

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Abstract

The invention relates to a calcium-complex starch-based microporous haemostatic material, and a preparation method and application thereof. The preparation method comprises the following steps of: firstly, preparing microporous starch loaded with carboxymethyl; secondly, complexing and assembling calcium ions; and finally, preparing the calcium-complex starch-based microporous haemostatic material, which has the pore diameter of 0.1-5um, the haemostatic time of 20 seconds to 30 minutes and can be completely degraded within 8 hours to 6 days. The cost of raw materials is low; the preparation method is simple; the obtained haemostatic material combines the physical haemostatic function of the microporous starch and the chemical haemostatic function of the carboxymethyl and the calcium ions, so that the haemostatic material is quick in haemostasis, high in efficiency, large in viscosity and good in biocompatibility, can be degraded in a body, and can be used for stopping bleeding of bleeding places such as human epidermis and internal tissues, massive bleeding places or active places of substantial organs in the body, and places where bleeding is difficult to control.

Description

technical field [0001] The invention belongs to the field of medical materials for repairing tissue damage, and in particular relates to a calcium complex starch-based microporous hemostatic material and its preparation method and application. Background technique [0002] Blood has the function of nourishing and moisturizing the tissues and organs of the whole body, and is one of the basic substances that constitute the human body and maintain human life activities. Traumatic hemorrhage is a common occurrence in human life, and acute massive hemorrhage and deep hemorrhage of substantial organs caused by war, traffic accidents, natural disasters, etc. are the main causes of death, disability and teratogenicity after human injury. When the traumatic blood loss reaches more than 20% of the total blood volume, obvious shock symptoms appear; when it reaches 40% of the total blood volume, there is life-threatening. According to reports, in the War to Resist US Aggression and Aid...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61L15/28A61L15/44A61L15/42C08B31/12
Inventor 刘昌胜陈芳萍陈晓龙魏杰
Owner 苏州佰济生物科技有限公司
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