Method for selenizing pilose asiabell root polysaccharides and application of product

A technology of codonopsis polysaccharides and selenization, which is applied in applications, medical preparations containing active ingredients, pharmaceutical formulas, etc., can solve the problem of low selenium content of codonopsis polysaccharides, and achieve the effect of improving anti-tumor activity and improving immune activity

Active Publication Date: 2012-07-04
LANZHOU UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] The purpose of the present invention is to solve the problem of low selenium content in Codonopsi

Method used

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  • Method for selenizing pilose asiabell root polysaccharides and application of product
  • Method for selenizing pilose asiabell root polysaccharides and application of product
  • Method for selenizing pilose asiabell root polysaccharides and application of product

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0022] Example 1 Extraction of Codonopsis polysaccharides

[0023] The polysaccharides of Codonopsis pilosula were extracted by water decoction-alcohol precipitation method. The specific process was as follows: take 1 kg of Codonopsis pilosula, crush them, put them in a round bottom flask, and reflux them twice with 85-95% (V / V) ethanol for 2 hours each time. After the slag is dried naturally, add 5 to 12 times the amount of water to decoct 4 times, 2 hours each time, combine the liquid medicine, concentrate in a water bath, and the concentration ratio is 4, let it stand overnight, discard the precipitate, add 95% ethanol to make the ethanol concentration reach 80%, stand overnight, centrifuge to obtain a precipitate, and then wash the precipitate with ethanol and dry to obtain crude Codonopsis polysaccharide. After the crude Codonopsis polysaccharide is depigmented and protein-removed, Codonopsis polysaccharide with a polysaccharide content of more than 50% is obtained.

Embodiment 2

[0024] Embodiment 2 Codonopsis polysaccharide modification

[0025] In this experiment, the influence of three factors including the mass ratio of selenous acid and Codonopsis polysaccharide, reaction time and reaction temperature on the selenization of Codonopsis polysaccharide was investigated. Using the Box-Behnken method, the selected three variables are the mass ratio of selenous acid to Codonopsis polysaccharide (X1), the reaction time (X2) and the reaction temperature (X3), according to the equation ci=(Xi-X0) / △X The independent variable is coded, where ci is the coded value of the variable, Xi is the real value of the variable, X0 is the real value of the variable at the center point of the experiment, and △X is the change step of the variable. The variables and their levels are shown in the table below

[0026]

[0027] Experimental process: dissolve Codonopsis polysaccharide in distilled water to make a saturated aqueous solution, add it to a three-necked bottle ...

Embodiment 3

[0036] Example 3 Biological activity of selenized Codonopsis polysaccharide product (Codonopsis selenium polysaccharide)

[0037] Codonopsis pilosula selenium polysaccharide was prepared according to the optimal selenization conditions determined in Example 2. The selenium content in the obtained product was 4906.6 μg / g, and was used for the following biological activity experiments.

[0038] (1) Improving immunity

[0039] Experimental animals: 6 six-week-old BALB / C mice

[0040] Experimental method: (1) Effect of codonopsis selenium polysaccharide on the proliferation of bone marrow cells in mice. The mice were killed by orbital bloodletting, and the bone marrow cells were taken out under aseptic conditions, and the cell suspension was made with complete culture medium DMEM, and counted to make the cell concentration reach 5 ×10 5 After culturing for 2 hours, centrifuge to remove the supernatant, add complete culture solution DMEM, then add lectin and Codonopsis selenium p...

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Abstract

The invention provides a method for selenizing pilose asiabell root polysaccharides. The method comprises the following steps of: adding selenous acid into an aqueous solution of the pilose asiabell root polysaccharides with stirring, adding a catalyst, reacting at the temperature of between 50 and 70 DEG C for 4 to 8 hours, cooling, separating, and purifying to obtain a selenized pilose asiabell root polysaccharide product, wherein the catalyst is barium chloride. By the method, the content of selenium in the pilose asiabell root polysaccharides can be improved to 500-5,000mu g/g, the anti-tumor activity and immunity improving activity of the selenized pilose asiabell root polysaccharides are obviously improved compared with those of non-selenized pilose asiabell root polysaccharides, and the product can be used for preparing anti-tumor medicines or medicines or health care products for improving immunity.

Description

technical field [0001] The invention belongs to the field of medicine synthesis, and in particular relates to a selenization method of codonopsis polysaccharide and its application. Background technique [0002] Codonopsis Codonopsis is a traditional Chinese medicine commonly used in traditional Chinese medicine. It is sweet in taste and flat in nature. Ginseng substitute. According to literature reports, the chemical composition contained in Codonopsis pilosula is relatively complex, including polysaccharides, lignin glycosides, phenolic acids, terpenes, steroids and so on. [0003] Codonopsis polysaccharide is one of the active ingredients in Codonopsis pilosula, and the traditional control and evaluation of the quality of Codonopsis pilosula is by measuring the content of polysaccharides. Modern pharmacological studies have confirmed that Codonopsis polysaccharides have various biological activities, among which anti-tumor, anti-oxidation and immunity-enhancing activiti...

Claims

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Application Information

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IPC IPC(8): C08B37/00A61K31/715A61P35/00A61P37/04A23L1/09
Inventor 胡芳弟
Owner LANZHOU UNIVERSITY
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