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Preparation method of lactic acid

A technology for inoculating lactic acid bacteria and lactic acid, which is applied in the field of biomass chemical engineering, can solve the problems of lack of efficient utilization methods and inability to use non-starch polysaccharides, and achieve the effects of low price, high compatibility and high productivity

Inactive Publication Date: 2012-07-04
BEIJING FORESTRY UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

Ethanol production using starch-based feedstock, the fermentation residue is rich in cellulose and yeast cells, often used as animal feed to increase income, however, this can only use yeast cells and not non-starch polysaccharides; if non-grain Raw materials, especially lignocellulosic raw materials for ethanol production, because the fermentation residue is rich in lignin, there is a lack of efficient utilization methods

Method used

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  • Preparation method of lactic acid

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Embodiment 1

[0032] Using 20% ​​corn hydrolyzate, adding 0.2% yeast, ethanol fermentation for 30h, the fermented liquid contains 27.6g / L sugar and 45.2g / L ethanol. Take 1L of the fermentation residue after the ethanol has been separated and heat it at 121°C for 60min. Lactic acid bacteria adaptive culture system contains furfural residue 5g / l, KH 2 PO 4 0.5g / l, MgSO 4 ·7H 2 O 0.5g / l, NaCl 0.1g / l and CaCO 3 20g / l, prepared with water. The culture temperature of the adaptive culture was 32°C, the culture time was 36 hours, and the pH value was 6.0. Take 0.17L of the treated fermentation residue and add it to the fermenter, add 5% (w / v) furfural slag for fermentation, make up the total fermentation volume to 1L with water, solid CaCO 3 5% (w / v), the addition of cellulase was 15FPU / g furfural residue, the addition of adaptive lactic acid bacteria was 1.25g / L, the fermentation temperature was 42°C, the pH value was 6.0, and the lactic acid was 31.0g / L after 120h, producing The ratio is...

Embodiment 2

[0034] Using 20% ​​corn hydrolyzate, adding 0.2% yeast, ethanol fermentation for 30h, the fermented liquid contains 27.6g / L sugar and 45.2g / L ethanol. Take 1L of the fermentation residue after the ethanol has been separated and heat it at 100°C for 10min. Lactic acid bacteria adaptive culture system contains furfural residue 5g / l, KH 2 PO 4 0.5g / l, MgSO 4 ·7H 2 O 0.5g / l, NaCl 0.1g / l and CaCO 3 20g / l, prepared with water. The culture temperature of the adaptive culture was 32°C, the culture time was 36 hours, and the pH value was 6.0. Take 0.17L of the treated fermentation residue and add it to the fermenter, add 5% furfural slag for fermentation, make up the total fermentation volume to 1L with water, solid CaCO 3 , 5% (w / v), cellulase addition 15FPU / g furfural slag, adaptive lactic acid bacteria addition 1.0g / L, fermentation temperature 38°C, pH 6.0, lactic acid after 108h was 27.9g / L, The yield was 89.9% (calculated based on the monosaccharide in the raffinate and th...

Embodiment 3

[0036] Using 20% ​​corn hydrolyzate, adding 0.2% yeast, ethanol fermentation for 30h, the fermented liquid contains 27.6g / L sugar and 45.2g / L ethanol. Add 1 mol / L hydrochloric acid to 1L of the fermented residue from which ethanol has been separated, place it at 25°C for 1 hour, heat at 100°C for 10 minutes, and then adjust it to neutral with 10% NaOH for later use. Lactic acid bacteria adaptive culture system contains furfural residue 5g / l, KH 2 PO 4 0.5g / l, MgSO 4 ·7H 2 O 0.5g / l, NaCl 0.1g / l and CaCO 3 20g / l, prepared with water. The culture temperature of the adaptive culture was 32°C, the culture time was 36 hours, and the pH value was 6.0. Take 0.17L of the treated fermentation residue and add it to the fermenter, add 5% (w / v) furfural slag for fermentation, make up the total fermentation volume to 1L with water, solid CaCO 3 , 5% (w / v), cellulase addition amount 20FPU / g furfural residue, adaptive lactic acid bacteria addition amount 1.5g / L, fermentation temperatur...

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Abstract

The invention provides a preparation method of lactic acid, which comprises the steps of taking an alcoholic fermentation residue of grains or cellulose materials and a furfural residue as raw materials; inoculating lactic acid bacteria; and adding cellulose, and fermenting to produce lactic acid. In the invention, the furfural residue rich in resources and loose in structure is taken as the cellulose materials and commercially available lactic acid bacteria are selected to prepare lactic acid by simultaneous saccharification and mixed fermentation by means of low cost alcoholic fermentation residue as a nitrogen source. The method couples alcoholic fermentation and lactic acid fermentation organically so as to improve the raw material utilization rate and reduce the consumption of chemicals.

Description

technical field [0001] The invention relates to the field of biomass chemical engineering, in particular to a new method for preparing lactic acid by fermenting ethanol fermentation residue and furfural residue as raw materials. Background technique [0002] Fossil refining can supply society's main energy and chemicals, while bringing serious environmental problems. With the continuous consumption of fossil raw materials and their non-renewability, the development of biomass refining is an inevitable trend to maintain sustainable social development. At present, the cost of biomass refining process remains high, and its commercial application is limited to the production of lactic acid and fuel ethanol, and they are all based on the refining process of grain raw materials such as starch. Fuel ethanol can directly replace petroleum fuels such as gasoline and diesel, and is an alternative energy source for fossil energy that is about to be exhausted; lactic acid is a multi-pu...

Claims

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Application Information

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IPC IPC(8): C12P7/56
Inventor 蒋建新唐勇冯月朱莉伟王堃
Owner BEIJING FORESTRY UNIVERSITY
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