Composition of pulmonary surfactant extract and pulmonary surfactant-associated protein A, preparation method and pharmaceutical application thereof

A technique for pulmonary surfactant and extract, which is applied to the composition, preparation and pharmaceutical application of pulmonary surfactant extract and pulmonary surfactant-related protein A, and can solve the problem of poor practicability, affecting the production of PS preparations, and inability to Realize industrialization and other issues to achieve the effect of improving activity and efficacy

Active Publication Date: 2013-11-06
BEIJING SHUANGHE RUNCHUANG TECH CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The former is not practical, will affect the production of PS preparations derived from animal lungs, and cannot be industrialized; the latter is only in the laboratory stage, and cannot obtain fully expressed SP-A, and there is still a long way to go before industrialization explore

Method used

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  • Composition of pulmonary surfactant extract and pulmonary surfactant-associated protein A, preparation method and pharmaceutical application thereof
  • Composition of pulmonary surfactant extract and pulmonary surfactant-associated protein A, preparation method and pharmaceutical application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0062] 1. Bovine lung surfactant extract

[0063] Self-made bovine lung lavage fluid 1000L, centrifuged at 500×g at a low speed, and the supernatant was concentrated and filtered at about 15°C with a ceramic microporous filter tube (pore size 300nm) at a working pressure of 0.1-0.3Mpa to contain 0.15 mol / The mixed solution of L sodium chloride and 1.5 mmol / L calcium chloride was equilibrated for 40 minutes, the concentration treatment time was 3 hours, and the same mixed solution was used as the eluent to circulate for 30 minutes to obtain 50 L of concentrated solution.

[0064] The concentrated solution was centrifuged at 12000×g, and the resulting precipitate was extracted with methanol / dichloroethane (volume ratio 1:2), and the extract containing surface-active phospholipids in the lower layer was separated for later use, and the remaining waste solution was used to prepare SP-A.

[0065] The extract was filtered and vacuum-dried to obtain a bovine lung surface active extr...

Embodiment 2

[0079] 1. Pig lung surfactant extract

[0080] Self-made porcine whole lung homogenate extract 800L, centrifuged at 1000×g at low speed, and the supernatant was concentrated and filtered at about 10°C with a ceramic microporous filter tube (pore size 50nm) at a working pressure of 0.1-0.3Mpa, with a concentration of 0.15 The mixed solution of mol / L sodium chloride and 15 mmol / L calcium chloride was equilibrated for 40 minutes, the concentration treatment time was 2.5 hours, and the same mixed solution was used as the eluent to circulate for 30 minutes to obtain 50 L of concentrated solution.

[0081] The concentrated solution was centrifuged at 10,000×g, and the resulting precipitate was extracted with n-hexane / chloroform (volume ratio 1:2), and the extract containing surface-active phospholipids in the lower layer was separated for later use, and the remaining waste solution was used to prepare SP-A.

[0082] The extract was vacuum-dried to obtain the porcine lung surface act...

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Abstract

The invention relates to a composition of pulmonary surfactant extract and pulmonary surfactant-associated protein A (SP-A), a pharmaceutical preparation and a preparation method thereof. The pulmonary surfactant extract is prepared by the methods of ceramic filtering pipe concentration and filtration, organic solvent extraction, purification and the like; at the same time, high-purity SP-A is prepared from the waste in the production process of the pulmonary surfactant extract; and the pulmonary surfactant extract and the SP-A are combined to obtain the pulmonary phospholipid SP-A composition. The composition has an antipathogenic activity inhibition effect; and the curative effect is better than that of the PS preparation without SP-A in treating the pulmonary surfactant deficiency or dysfunction diseases such as acute respiratory distress syndrome and chronic obstructive pulmonary diseases caused by pneumonia, meconium aspiration syndrome, bronchopulmonary dysplasia, infant extracorporeal circulation, lung injury and the like.

Description

technical field [0001] The present invention relates to the composition of lung surfactant extract and pulmonary surfactant-associated protein A (surfactant associated protein A, SP-A), which includes three parts: (1) the composition of lung surfactant extract and SP-A; (2) The preparation method of the composition; (3) the composition is used in the treatment of pulmonary surfactant deficiency or dysfunction diseases, such as acute respiratory syndrome caused by pneumonia, meconium aspiration syndrome, bronchopulmonary dysplasia, extracorporeal circulation in infants and young children, lung injury, etc. Pharmaceutical use in distress syndrome and chronic obstructive pulmonary disease. [0002] The invention is suitable for industrialized production. Background technique [0003] Pulmonary surfactant (PS) is a phospholipid-protein complex secreted by alveolar type II cells, which can reduce the surface tension of alveolar air-liquid interface, prevent atelectasis and pulmo...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61K38/17A61K9/10A61K9/19A61P11/00C07K14/785C07K1/36C07K1/34C07K1/16C07K1/22A61K35/42
Inventor 于顺廷寇同欣陆文超赵海英秦晓君胡金艳王梦德
Owner BEIJING SHUANGHE RUNCHUANG TECH CO LTD
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