Gel composition for injection filling of skins and preparation method for gel composition

A gel composition and gel technology, applied in the field of gel composition and its preparation, can solve the problems of increasing the risk of organic solvents, not involving microgels of various particle size levels, and complicated preparation processes, etc.

Inactive Publication Date: 2012-07-11
上海白衣缘生物工程有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

For the preparation method of cross-linked HA microgel, there are many patents or patent applications involved, such as CN101502677A, CN101244290A and CN102188712A, etc., but these methods are all the preparation of a particle size grade microgel, and do not involve a method Preparation of microgel technology with various particle size levels
Patent application WO2005067994 (CN1893989A) involves the technology of preparing microgels with various particle sizes, but this technology uses steps such as organic solvent precipitation and washing, which increases the risk of residual organic solvents in the product, and the preparation process is relatively complicated

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  • Gel composition for injection filling of skins and preparation method for gel composition
  • Gel composition for injection filling of skins and preparation method for gel composition

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preparation example Construction

[0038] The preparation process of cross-linked HA gel particles adopts simple and feasible processes such as freezing, crushing and sieving, and easily obtains microgels of different particle size grades, among which the small particle size grade gel is suitable for injection into the superficial layer of the skin dermis , Medium particle size gel is suitable for injection into the middle to deep layer of the skin dermis, and large particle size gel is suitable for injection into the subcutaneous layer, both of which have the cosmetic effect of filling wrinkles and repairing depressions. Therefore, the present invention is easy to prepare a series of gel compositions suitable for injection filling of different layers of skin.

[0039] Due to the use of weakly alkaline natural HA solution mixed with gel ice particles, the gel composition prepared by the present invention contains a small amount of natural HA, which can reduce the injection resistance when injecting the product. ...

Embodiment 1

[0056] 5.0g hyaluronic acid (HA) raw material (molecular weight is 1 million Daltons), dissolved in 40ml of 0.2N NaOH solution, after dissolving, add 3ml of 1,4 butanediol diglycidyl ether, fully stir, place in 37 °C incubator for 4 hours, the cross-linked HA gel block is formed. The gel block was washed with distilled water and dialyzed, and the dialysate was changed 6 times in the following 3 days. Remove the distilled water, freeze the cross-linked HA gel block in a -50°C freezer for 24 hours, take out the cross-linked HA ice cube, set the temperature of the micronizer at -20°C in an environment of 4°C, and use the micronizer to quickly crush the ice cubes. Then pass through stainless steel mesh sieves with an average pore size of 200 μm, 500 μm, 1000 μm and 1500 μm in sequence, and collect gel ice particles with different particle size levels respectively. Add 50 ml of solution with pH 8.5, HA concentration 4%, and NaCl concentration 1.5% per 100 g of cross-linked HA ice ...

Embodiment 2

[0058] 5.0g of HA raw material (molecular weight is 1 million Daltons), dissolved in 50ml of 0.2N NaOH solution, after dissolving, add 4ml of 1,4 butanediol diglycidyl ether, fully stir, and place in a 32°C thermostat for 6 hours, That is, a cross-linked HA gel block is formed. The gel block was washed and dialyzed with distilled water, and the dialysate was changed 9 times in the next 3 days. Remove the distilled water, freeze the cross-linked HA gel block in a -50°C freezer for 48 hours, take out the cross-linked HA ice cube, set the temperature of the micronizer at -15°C in an environment of 4°C, and use the micronizer to quickly crush the ice cubes. Then pass through stainless steel mesh sieves with an average pore size of 200 μm, 500 μm, 1000 μm and 1500 μm in sequence, and collect gel ice particles with different particle size levels respectively. Add 50ml of solution with pH 8.9, HA concentration 3.5%, and NaCl concentration 1.5% per 100g of cross-linked HA ice particl...

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Abstract

The invention discloses a method for preparing a gel composition for injection filling of skins. The method comprises the following steps of: performing cross-linking reaction on a hyaluronic acid raw material and a cross-linking agent to obtain a gel block, and cleaning and dialyzing the gel block; freezing the dialyzed gel block into a gel ice block, crushing by using a micro-pulverizer, and collecting gel ice particles with different particle size ranges by using a series of mesh sieves with different apertures; and swelling the gel ice particles with different particle size ranges by using a weakly alkaline hyaluronic acid solution respectively so as to close a residual free-state end of the cross-linking agent, and adding an acid solution to obtain the gel composition of which the pH value is within a physiologically acceptable range. By the method, the problem about the residue of the cross-linking agent of which one end is in a bonding state and the other end is still a free-state functional group after cross-linking reaction is solved; and the microgel composition with different particle size levels can be obtained by a simple preparation process so as to be suitable for the injection on different layers of the skins and achieve the beauty effects of filling, removing wrinkles and repairing depressions.

Description

technical field [0001] The invention relates to a gel composition suitable for skin injection filling and a preparation method thereof, in particular to the preparation of a series of gel compositions with different particle sizes suitable for injection at different layers of the skin. Background technique [0002] Hyaluronic acid (HA) is a linear polymer polysaccharide composed of glucuronic acid and acetylglucosamine disaccharides combined with each other. It is an important component of skin, vitreous body, synovial fluid and cartilage tissue. HA is a physiological substance with high viscoelasticity, good biocompatibility, non-immunogenicity, and no species specificity, so it has been widely used in the medical and beauty industries. At present, HA products are mainly used as ophthalmic viscoelastic agents, orthopedic viscoelastic supplements, and postoperative anti-adhesion agents. Polymer HA solutions can also be used as dermal fillers. After injection into the skin, i...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61L27/20C08J3/075C08J3/24
Inventor 韩韦红
Owner 上海白衣缘生物工程有限公司
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