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Chitosan base expanded bed adsorption medium with double function ligands and preparation method thereof

A technology of expanded bed adsorption and chitosan, which is applied in the field of expanded bed adsorption and separation of proteins in chromatographic separation, expanded bed adsorption medium and preparation, can solve the problems of narrow operating range of flow rate, high price, constraints, etc., and achieve the scale of promotion The effects of chemical separation and purification, improvement of adsorption separation efficiency and selectivity

Inactive Publication Date: 2013-10-30
SHENYANG INSTITUTE OF CHEMICAL TECHNOLOGY
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

But its high price restricts its application in China. In addition, the Streamline series resin still has the problem of narrow operating range of flow rate.

Method used

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  • Chitosan base expanded bed adsorption medium with double function ligands and preparation method thereof

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0021] Mix 100ml of chitosan solution with a mass fraction of 5% with 20g of stainless steel powder and 3g of PEG2000, then add 100ml of liquid paraffin, 15 drops of span-80, stir at high speed for 30min to make the system into an emulsion, then add 11ml of formaldehyde, and stir for 1h Pour the emulsion into a mixture of 10% NaOH / dehydrated ethanol (V(NaOH):V(dehydrated ethanol)=1:1) and let it stand for 2 hours, then wash it thoroughly with xylene, dehydrated ethanol and deionized water. Sieve to obtain chitosan / stainless steel powder composite microspheres with a particle size of 50-300 μm as a matrix; mix 10 ml of drained composite microspheres, 50 ml of methanol, and 5 ml of benzaldehyde, react in a shaker at 200 rpm at 60 ° C for 12 h, and then add React sodium borocyanide at room temperature for 12 hours, filter with suction, and wash with deionized water to obtain the benzaldehyde matrix; then mix the benzaldehyde matrix, 100ml of 0.5mol / L NaOH solution, and 20ml of epi...

Embodiment 2

[0023]Mix 100ml of chitosan solution with a mass fraction of 5% with 35g of stainless steel powder and 3g of PEG2000, then add 100ml of liquid paraffin, 15 drops of span-80, stir at high speed for 30min to make the system into an emulsion, then add 11ml of formaldehyde, and stir for 1h Pour the emulsion into a mixture of 10% NaOH / dehydrated ethanol (V(NaOH):V(dehydrated ethanol)=1:1) and let it stand for 2 hours, then wash it thoroughly with xylene, dehydrated ethanol and deionized water. Sieve to obtain chitosan / stainless steel powder composite microspheres with a particle size of 50-300 μm as a matrix; mix 10ml of drained composite microspheres, 50ml of methanol, and 5ml of benzaldehyde, react in a shaker at 200rpm at 60°C for 8h, and then add React sodium borocyanide at room temperature for 12 hours, filter with suction, and wash with deionized water to obtain the benzaldehyde matrix; then mix the benzaldehyde matrix, 100ml of 0.5mol / L NaOH solution, and 20ml of epichlorohyd...

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Abstract

A chitosan base expanded bed adsorption medium with double function ligands and a preparation method thereof relate to an adsorption medium and a preparation method thereof. A matrix is a chitosan / stainless steel powder composite microsphere, and the ligands are phenyl introduced after benzaldehyde activation and iminodiacetic acid coupled after epoxy chloropropane activation. An inverse phase suspension crosslinking method is utilized to prepare the chitosan / stainless steel powder composite microsphere serving as a blank matrix. A method for respectively designing two ligands is utilized, firstly a microsphere matrix C2-benzene is formalized to construct a hydrophobic interaction ligand, then a matrix C6-hydroxide radical is activated by the epoxy chloropropane and is coupled with the iminodiacetic acid, and the expanded bed adsorption medium with the phenyl and the epoxy chloropropane serving as the ligands and used for separating proteins is obtained. The novel expanded bed adsorption medium simultaneously has a metal chelating affinity ligand and a hydrophobic interaction ligand, improves adsorption separation efficiency and selectivity of target proteins in feed liquid, and can be applied to large-scale purification of the proteins.

Description

technical field [0001] The present invention relates to an adsorption medium and its preparation method, in particular to an expanded bed adsorption medium for protein separation using phenyl and iminodiacetic acid as ligands and its preparation method, which belongs to the expansion bed adsorption separation in chromatographic separation protein technology. Background technique [0002] Bioseparation technology is an important part of bioengineering technology. Improving bioseparation technology, purifying target products with low cost, high yield, and high efficiency, and effectively controlling the content of harmful impurities are the key to whether biologically active substances can be transformed into products with practical value. and competitive products are the primary issues. [0003] Expanded bed adsorption (EBA) is a new type of protein separation operation technology, which was formed in the 1990s and has been widely used in the field of bioengineering in recen...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): B01J20/24B01J20/30C07K1/22
Inventor 朱建星王红艳
Owner SHENYANG INSTITUTE OF CHEMICAL TECHNOLOGY