Method for identifying salt tolerance of cotton

An identification method and a technology of salt tolerance, which are applied in horticultural methods, botanical equipment and methods, horticulture, etc., can solve the problems of long identification time, large floor area, and low efflux capacity, so as to reduce the pre-cultivation time, The effect of reducing errors and avoiding errors

Inactive Publication Date: 2012-07-18
SHANDONG COTTON RES CENT
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Problems solved by technology

In 2011, Cuin et al. used non-damaging micro-measurement technology to study and found that Na in the roots of salt-tolerant wheat cultivars under salt stress + The efflux capacity is higher than that of salt-sensitive wheat cultivars, but K + Efflux capacity is lower than that of salt-sensitive wheat varieties
At present, the identification of cotton salt

Method used

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  • Method for identifying salt tolerance of cotton
  • Method for identifying salt tolerance of cotton

Examples

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Example Embodiment

[0018] Example 1:

[0019] Take three sterilized petri dishes with a radius of 10 cm, put them into two filter papers of the same size, and add 10 ml of sterilized double distilled water; combine Simian No. 3 (SM3) and the two over-expressing spinach CMO genes Salt-tolerant cotton strains (CMO2 and CMO4) each have 20 lint-free seeds. After soaking, peel off the seed coat, disinfect with 0.1% mercury liter solution for 15 minutes, then rinse with sterilized double distilled water for 6 times, and plant them in the above-mentioned culture. In a dish, put it in a greenhouse (22~32℃) to germinate and emerge. After the cotyledons of cotton seedlings are fully expanded, select 10 robust cotton seedlings of the same size for each variety and place them in a nursery bowl filled with Hoagland nutrient solution (22~32℃, humidity 60%-75%). Change every day. After the cotton grows a true leaf, 3 cotton seedlings with complete roots and the same size are selected from each line and treated ...

Example Embodiment

[0023] Example 2:

[0024] Take two sterilized petri dishes with a radius of 10cm, put them into two filter papers of the same size, and add 10ml of sterilized double distilled water; put the full and plump cotton (Lumianyan No. 28) and the seeds of seaside mallow Each 20 grains, peel off the seed coat, soak in 0.1% mercury solution for 15 minutes, then rinse with sterilized double distilled water 6 times, plant them in the above-mentioned petri dishes, and place them in a greenhouse (22-32°C) to germinate and emerge. After cotton (Lumianyan No. 28) and mallow cotyledons are fully expanded, select 10 robust seedlings of the same size and place them in a seedling bowl filled with Hoagland nutrient solution (22~32℃, humidity 60%~75%) ), the nutrient solution is changed every two days. After one true leaf grows, three seedlings with complete roots and the same size are selected and treated with Hoagland nutrient solution containing 150mM NaCl for 24 hours.

[0025] Table 2 Na of roo...

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Abstract

The invention discloses a method for identifying salt tolerance of cotton, comprising the following steps: selecting a robust cotton seedling with one true leave, treating the cotton seedling with a nutrient solution containing 150mM of NaCl for 24h, selecting a lateral root in a good growth state, cutting off the point of the lateral root, pre-treating the point of the lateral root in a test buffer solution for 30min, fixing the point of the lateral root in a culture dish filled with a fresh test solution, testing the point of the lateral root by using a non-invasive micro-test system, analyzing data by using Mageflux software to obtain an efflux velocity of Na<+> and an efflux velocity of K<+>, and calculating to obtain an efflux velocity ratio of Na<+>/K<+>. The higher the efflux velocity of the Na<+>, the lower the efflux velocity of the K<+>, or the higher the efflux velocity ratio of the Na<+> to the K<+>, the higher the salt tolerance of the cotton. The method for identifying the salt tolerance of the cotton has the characteristics of non-invasive detection, accurate data and rapid identification, can be used for identifying the salt tolerance of cotton and can be also used for salt tolerance of other plants.

Description

technical field [0001] The invention relates to a method for identifying the salt tolerance of cotton, specifically measuring the Na in cotton roots under salt stress by using a non-damaging micro-measurement technique. + and K + Flow rate, a method for identifying salt tolerance in cotton. Background technique [0002] In 1974, the neuroscientist Jaffe of the American Marine Biology Laboratory first proposed the original concept of non-invasive micro-measurement technology. In 1990, the non-invasive micro-measurement technology was successfully applied to the determination of Ca in cells. 2+ At present, this technology has been widely used in the fields of life science, environmental science and material science. Ca 2+ 、H + 、K + 、Na + , Cl - The information of plasma flow velocity and three-dimensional motion direction has the characteristics of living body, dynamic, real-time, internal and external measurement, long-term, multi-dimensional scanning and measurement. ...

Claims

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Application Information

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IPC IPC(8): A01G7/00
Inventor 董合忠孔祥强罗振代建龙李维江辛承松唐薇张冬梅
Owner SHANDONG COTTON RES CENT
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