Yeast culture material, preparation method of yeast culture material and application of yeast culture material in improving rumen fermentation

A technology of yeast culture and fermentation liquid, which is applied in the application field of yeast culture and its preparation and improvement of rumen fermentation, which can solve the problems of metabolite composition and concentration differences, and achieve the effect of improving productivity and optimizing nutritional value

Inactive Publication Date: 2014-12-10
CHINA AGRI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Even when the same yeast strain is used, different medium compositions or different fermentation production control processes can lead to significant differences in the composition and concentration of metabolites in yeast culture products

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0040] Embodiment 1, yeast culture and its preparation method and application

[0041] 1. Preparation of Yeast Culture

[0042] 1. Preparation of culture medium

[0043] Liquid seed medium: weigh 10g glucose, 5g yeast powder, 5g peptone and 0.1g MnSO 4 ·H 2 O, after heating and dissolving with 1L of distilled water, sterilize at 121°C and 0.1Mpa for 15min, and set aside.

[0044]Liquid fermentation medium: weigh 10g glucose, 10g sucrose, 10g molasses, 10g peptone, 10g yeast extract, 5g ammonium sulfate, 0.1g KH 2 PO 4 , 0.1g MgSO 4 ·7H 2 O, 0.1g MnSO 4 ·H 2 O, 0.1g CaCl 2 , 0.1gCuSO 4 ·5H 2 O, 0.1g ZnSO 4 ·7H 2 O and 0.1 g FeSO 4 4H 2 O, after dissolving in distilled water, adjust the pH value to 4.0 with 0.1mol / L HCl solution, dilute to 1L, sterilize at 121°C, 0.1Mpa for 15min, and set aside.

[0045] 2. Obtaining of seed solution

[0046] The freeze-dried and preserved Saccharomyces cerevisiae (Saccharomyces cerevisiae CGMCC No.2.1882) was activated for thre...

Embodiment 2

[0112] Embodiment 2, yeast culture and its preparation method and application

[0113] 1. Preparation of Yeast Culture

[0114] 1. Preparation of culture medium

[0115] Liquid seed medium: weigh 40g glucose, 40g yeast powder, 40g peptone and 4.0g MnSO 4 ·H 2 O, after heating and dissolving with 1L of distilled water, sterilize at 121°C and 0.1Mpa for 15min, and set aside.

[0116] Liquid fermentation medium: 100g glucose, 100g sucrose, 100g molasses, 300g peptone, 300g yeast extract, 40g ammonium sulfate, 10g KH 2 PO 4 , 10g MgSO 4 ·7H 2 O, 4.0g MnSO 4 ·H 2 O, 4.0 g CaCl 2 , 4.0g CuSO 4 ·5H 2 O, 4.0g ZnSO 4 ·7H 2 O and 4.0 g FeSO 4 4H 2 O, after dissolving in distilled water, adjust the pH value to 6.5 with 0.1mol / L NaOH solution, set the volume to 1L, sterilize at 121°C and 0.1Mpa for 15min, and set aside.

[0117] 2. Obtaining of seed solution

[0118]The freeze-dried and preserved Saccharomyces cerevisiae (Saccharomyces cerevisiae CGMCC No.2.1882) was acti...

Embodiment 3

[0138] Embodiment 3, yeast culture and its preparation method and application

[0139] 1. Preparation of Yeast Culture

[0140] 1. Preparation of culture medium

[0141] Liquid seed medium: 20g glucose, 20g yeast powder, 20g peptone and 2.0g MnSO 4 ·H 2 O.

[0142] Liquid fermentation medium: 50g glucose, 50g sucrose, 50g molasses, 50g peptone, 50g yeast extract, 20g ammonium sulfate, 5.0g KH 2 PO 4 , 5.0 g MgSO 4 ·7H 2 O, 2.0g MnSO 4 ·H 2 O, 2.0g CaCl 2 , 2.0 g CuSO 4 ·5H 2 O, 2.0g ZnSO 4 ·7H 2 O and 2.0 g FeSO 4 4H 2 O, after dissolving in distilled water, adjust the pH value to 5.5 with 0.1mol / L NaOH solution, set the volume to 1L, sterilize at 121°C and 0.1Mpa for 15min, and set aside.

[0143] 2. Obtaining of seed solution

[0144] The freeze-dried and preserved Saccharomyces cerevisiae (Saccharomyces cerevisiae CGMCC No.2.1882) was activated for three generations and then inserted into the above-mentioned liquid seed medium, and cultured with shaking at ...

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PUM

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Abstract

The invention discloses a yeast culture material, a preparation method of the yeast culture material and application of the yeast culture material in improving rumen fermentation. The yeast culture material is prepared by the method comprising the following steps of: performing liquid fermentation on Saccharomyces cerevisiae CGMCC (China General Microbiological Culture Collection Center) No. 2.1882, collecting fermentation liquid, and removing the Saccharomyces cerevisiae cells from the fermentation liquid to obtain a aseptic liquid, namely the yeast culture material. By using the yeast culture material provided by the invention to perform in-vitro culture on ruminant rumen liquor, lactic acid utilization bacteria and cellulose decomposition bacteria, the ammonia nitrogen concentration and volatile fatty acid content in the ruminant rumen liquor are improved by 38.3-65.58% and 29.8-39.4%, respectively, and the proliferation of the lactic acid utilization bacteria and cellulose decomposition bacteria are significantly promoted. The yeast culture material has important significance in improving the productivity level of ruminants and optimizing the nutritive value of feed.

Description

technical field [0001] The invention relates to a yeast culture, its preparation method and its application in improving rumen fermentation. Background technique [0002] With the popularization and even abuse of feed antibiotics, the negative effects such as drug resistance and drug residues have become increasingly prominent. The European Union has banned antibiotics as feed additives since 2006; the United States has almost frozen the approval of all new antibiotics in recent years; the Chinese government has also promulgated a series of regulations on the use of feed drug additives. The fading out of feed antibiotics is an inevitable trend in the development of animal husbandry. The development and utilization can selectively promote the proliferation of intestinal beneficial bacteria, improve the intestinal environment, and improve the immunity of animals. At the same time, they will not be decomposed by intestinal enzymes and will not produce drug resistance. Non-toxi...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12P1/02A23K1/16C12R1/865
Inventor 刘萍解洛香徐乐
Owner CHINA AGRI UNIV
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