Rapid propagation method of Bletilla striata

A Bletilla striata and rapid technology, applied in the field of bioengineering, can solve the problems of successful rapid propagation of Bletilla striata tissue culture and other problems, and achieve the effects of eliminating the infestation of diseases and insect pests and pesticide residues, high application value and simple method.

Inactive Publication Date: 2012-07-25
向华
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] So far, there have been no reports on the success of blet

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0027] Material: Bletilla striata stems with buds as explants

[0028] 1. Selection and sterilization of explants: The tender tissues such as fresh shoots of Bletilla striata from Cili, Hunan are used as culture materials, washed with detergent and tap water, and rinsed under running water for 10-30 minutes. Place it in 75% alcohol for 10-30 seconds, then sterilize with 0.1% mercury liters for 10 minutes, rinse with sterile water, and cut off the discolored part of the explant for use.

[0029] 2. Germination of buds: inoculate the sterilized buds on LS+6-BA1.2mg / L+NAA0.2mg / L medium, pH value 5.8, culture temperature 23-27℃, light every day 12 hours, the light intensity is 1000LX; the buds will germinate 6-8 days after inoculation, and the seedlings with leaves will be formed in about 10 days.

[0030] 3. Induction and proliferation of clump buds: cut the seedlings and transfer them to LS+6-BA0.5mg / L+IAA0.4mg / L medium until clump buds are formed, the pH is 5.8, and the culture tempe...

Embodiment 2

[0035] Material: Bletilla striata stems with buds as explants

[0036] 1. Selection and sterilization of explants: The tender tissues such as fresh shoots of Bletilla striata from Cili, Hunan are used as culture materials, washed with detergent and tap water, and rinsed under running water for 10-30 minutes. Place it in 75% alcohol for 10-30 seconds, then sterilize with 0.1% mercury liters for 10 minutes, rinse with sterile water, and cut off the discolored part of the explant for use.

[0037] 2. Germination of buds: inoculate the sterilized buds on LS+6-BA1.2mg / L+NAA0.3mg / L medium, pH 5.8, culture temperature 23-27℃, light every day 12 hours, the light intensity is 1000LX; the buds will germinate 7-8 days after inoculation, and the seedlings with leaves will be formed about 12 days.

[0038] 3. Induction and proliferation of clump buds: cut the seedlings and transfer them to LS+6-BA0.5mg / L+IAA0.4mg / L medium until clump buds are formed, the pH is 5.8, and the culture temperature is...

Embodiment 3

[0043] Material: Bletilla striata stems with buds as explants

[0044] 1. Selection and sterilization of explants: The tender tissues such as fresh shoots of Bletilla striata from Cili, Hunan are used as culture materials, washed with detergent and tap water, and rinsed under running water for 10-30 minutes. Place it in 75% alcohol for 10-30 seconds, then sterilize with 0.1% mercury liters for 10 minutes, rinse with sterile water, and cut off the discolored part of the explant for use.

[0045] 2. Germination of buds: inoculate the sterilized buds on LS+6-BA1.2mg / L+NAA0.1mg / L medium, pH 5.8, culture temperature 23-27℃, light every day 12 hours, the light intensity is 1000LX; about 6-8 days after inoculation, the buds will germinate, and the seedlings with leaves will be formed about 10 days.

[0046] 3. Induction and proliferation of clump buds: cut the seedlings and transfer them to LS+6-BA0.5mg / L+IAA0.4mg / L medium until clump buds are formed, the pH is 5.8, and the culture tempera...

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PUM

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Abstract

The application relates to a rapid propagation method of Bletilla striata. The method comprises the following steps: firstly culturing a pseudobulb section and other tissues of Bletilla striata on an MS (Murashige and Skoog) culture medium, cutting a rootstock section with 1-2 buds, which is served as an explant; disinfecting, and inoculating in a culture medium of MS+6-BA (0.5mg/L)+NAA (0.4mg/L) and culturing, wherein the buds germinate about 6-8 days after inoculation and grow into seedlings with leaves about 10 days after inoculation; cutting down the seedlings and transferring to a culture medium of MS+6-BA (1.5mg/L)+IAA (0.4mg/L) so as to maintain high-speed multiplication, wherein the multiplication coefficient is higher than 5; using MS+6-BA (1.5mg/L)+IAA (0.4mg/L)+NAA (0.5mg/L) as a rooting culture medium, wherein the rooting rate is higher than 90% and the seedlings can grow into complete plants only about 25 days; and transplanting test-tube plantlets into sandy soil and maintaining proper water content and moisture, wherein transplanting survival rate is higher than 90% one month after transplanting and a set of high-frequency stable regeneration system is established. The method disclosed by the invention has the advantages of being good in stability, simple and convenient in operation, high in propagation speed, low in production cost and reaching the industrialization level and the like.

Description

Technical field [0001] The invention relates to the field of bioengineering, and relates to plant tissue culture technology, in particular to a tissue culture method of Bletilla striata. Background technique [0002] Plants are a natural treasure house of medicines. People have a long history of using medicinal plants. About 75% of the world's population use plants as a source of medicines for the treatment and prevention of diseases (Xing Jianmin, 2001). Mankind has discovered many drugs with high physiological activity from plants. Currently, drugs derived from plants account for more than 25% of the total drugs (Zheng Guangzhi, 1987). Traditional Chinese herbal medicine in my country has a history of thousands of years and is still widely used in my country and many countries and regions. However, traditional Chinese herbal medicines are obtained at the cost of collecting and consuming a large amount of wild plant resources. When the amount of collection and consumption excee...

Claims

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Application Information

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IPC IPC(8): A01H4/00
Inventor 向华
Owner 向华
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