Method for extracting active ingredients of Euphorbia fischeriana and diterpenoid medicament prepared by active ingredients

A technology of Euphorbia chamaejasma and its active ingredients, which is applied in the field of extracting active ingredients of Euphorbia chamaejasma, can solve the problems of low extraction rate of diterpenoids and difficulty in improving the therapeutic effect, and achieve simple economy, large processing capacity, and easy industrialization Effect

Inactive Publication Date: 2012-07-25
QIQIHAR MEDICAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

These methods have a low extraction rate of diterpenoids and are difficult to improve the therapeutic effect

Method used

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  • Method for extracting active ingredients of Euphorbia fischeriana and diterpenoid medicament prepared by active ingredients

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0022] Take the root of Euphorbia chamaejasma, dry it in the shade, grind it into 14-24 mesh powder, put it into a supercritical carbon dioxide extraction kettle, extraction conditions: 25 MPa, 55°C, supercritical carbon dioxide fluid flow rate 100:10% (v / v ) ratio to add entrainer absolute ethanol. Separation tank separation: 8 MPa, 40°C. Extract according to the above conditions for 4 h, and collect the extract separated from the separation tank.

[0023] Ethanol was recovered from the extract under reduced pressure until there was no distillate and a white viscous substance was obtained. This white viscous thing is made into suspension with sherwood oil: ethyl acetate (75:25), and this liquid flows through the macroporous adsorption resin column (diameter 5cm, length 100cm), to about 1 / 3 column volume of the colored layer. First use petroleum ether:ethyl acetate (75:25) to elute 3 times column volume at a flow rate of 3BV / h; Column volume, collect the eluate containing...

Embodiment 2

[0029] Take the root of Euphorbia chamaejasma, dry in the shade, and crush it into 14-24 mesh powder. Get the same amount of medicinal powder and put it into a supercritical carbon dioxide extraction kettle, and extract according to the supercritical carbon dioxide extraction conditions listed in Table 1. The supercritical carbon dioxide fluid extraction volume is 8 times the volume of the extraction kettle. Carry out macroporous resin separation then, the eluent containing compound jolkinolide B component in the obtained product, it is named as compound 1, contains the eluent of compound 17-hydroxyjolkinolide B component, is named as compound 2, contains The eluate of component A of compound 17-hydroxyjolkinolide, which is named as compound 3, is listed in Table 1 relative to the content of the drug powder.

[0030] Table 1

[0031]

Embodiment 3

[0033] The preparation of medicine of the present invention:

[0034] The three enriched products in Example 1 were combined according to the following ratios to form the diterpene drug ETD516 (1).

[0035] Jolkinolide B enrichment: 17-hydroxyjolkinolide B enrichment: 17-hydroxyjolkinolide A enrichment==25:30:45

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Abstract

The invention discloses a method for extracting active ingredients of Euphorbia fischeriana and a diterpenoid medicament prepared by the active ingredients. The method comprises the following steps of: taking a root of the Euphorbia fischeriana; extracting by adopting a supercritical carbon dioxide extraction method and taking absolute ethyl alcohol as an entrainer; and separating the obtained extractive by macroporous adsorption resin to obtain a diterpenoid active substance having medical curative effect. As supercritical carbon dioxide extraction is adopted, the extraction efficiency is high, the processing capacity is large, certain selectivity can be obtained through setting of extraction parameters, carbon dioxide can be recycled, the environment is not polluted, conditions are mild, natural activity of a product is propitious to be kept, and the like. The macroporous adsorption resin separation has large processing capacity, mainly takes water and ethanol as solvents, is economic, simple and convenient, is easy to industrialize, and the like.

Description

technical field [0001] The invention relates to a method for extracting active ingredients of Euphorbia chamaejasma, and also relates to a diterpene drug prepared from the active ingredient, which is mainly used for treating breast tumors. Background technique [0002] Malignant tumor is a common and frequently-occurring disease that seriously endangers human life and health. It is also an important killer that endangers human life and health. Currently, about 90% of malignant tumor patients die of tumor metastasis. Controlling metastasis is a key factor in determining the prognosis of malignant tumor patients. Clinical treatment of malignant tumors mainly adopts surgical resection, radiotherapy and chemical drug treatment, but these treatments are not effective in controlling tumor metastasis. About 30% of newly diagnosed solid tumor patients have clinically visible metastases, while The currently discovered anti-tumor metastasis agents such as retinamide, paclitaxel, arabi...

Claims

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Application Information

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IPC IPC(8): C07D493/20C07D493/10A61K31/365A61P35/00
CPCY02P20/54
Inventor 刘吉成王晓丽崔红霞牛英才李晓明
Owner QIQIHAR MEDICAL UNIVERSITY
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