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High-amplification multifunctional serum-free medium for immunocyte treatment and preparation method thereof

A technology for immune cell therapy and serum-free culture medium, which is applied in the fields of biotherapy and cell biology technology and materials, can solve the problems of easy-to-grow bacteria, affect the quality of culture medium, cannot be sterilized by steam, etc., and achieve convenient source. Effect

Inactive Publication Date: 2012-07-25
MEIDE TAIPINGYANG PRECISION INSTR MFG
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Problems solved by technology

[0008] (2) There are several "heat-labile" proteins in the tissue. If they are not removed, precipitates will appear during cultivation at 37°C, which will seriously affect the quality of the medium.
[0009] (3) Serum-free cell culture medium contains biologically active substances, which cannot be sterilized by steam, while serum-free cell culture medium is rich in nutrients and easy to grow bacteria

Method used

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Examples

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Embodiment Construction

[0045] Below in conjunction with the examples, the present invention is further described, the following examples are illustrative, not limiting, and the protection scope of the present invention cannot be limited by the following examples.

[0046] 1. The technical principles used in the production of culture medium:

[0047] The medium uses inorganic salts, amino acids, vitamins and other substances to prepare liquid nutrients, adjusts its pH value at room temperature, is sterilized by nano-scale filtration, and is packaged in a sterile environment.

[0048] Second, the specific preparation method of the culture medium:

[0049]1. Composition

[0050] a) Amino Acids Each liter of the product contains the following amino acids:

[0051] Glycine 30mg, L-alanine 25mg, L-arginine hydrochloride 84mg, L-asparagine 25mg, L-aspartic acid 30mg, L-cystine 70mg, L-glutamic acid 75mg, L -Glutamine 584mg, L-histidine hydrochloride 42mg, L-isoleucine 105mg, L-leucine 105mg, L-lysine hy...

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Abstract

The invention relates to a high-amplification multifunctional serum-free medium for immunocyte treatment and a preparation method of the high-amplification multifunctional serum-free medium. The components of the medium comprise amino acids, vitamins, inorganic salts and other components, and the components are added in contents according to the component range. The preparation method comprises the steps of mixing, adjusting pH and finally filtering and sterilizing. Compared with similar products at home and abroad, the serum-free cell medium provided by the invention has the performance indexes completely reaching the level of similar products, and with respect to the use condition, the serum-free cell medium also completely meets the requirements of users and reaches the level of similar products at home and abroad; and in addition, the resources of materials for domestic product are convenient.

Description

technical field [0001] The invention belongs to the technical and material fields of biotherapy and cell biology, in particular to a highly amplified multifunctional serum-free medium for immune cell therapy and a preparation method thereof. Background technique [0002] In the research field of biomedical engineering on cells and the clinical application of medicine, the main purpose is to study the functions and effects of cells. [0003] Malignant tumors are diseases that seriously endanger human health. The three conventional therapies of "surgery, radiotherapy and chemotherapy" still cannot solve the recurrence and metastasis of tumors. Practice has proved that tumor biological immunotherapy can well control tumor metastasis and recurrence. Treatment (surgery, radiotherapy, chemotherapy) combined with comprehensive therapy of tumor immunotherapy will become the main tool for human beings to conquer cancer in the 21st century. [0004] Somatic cell technology is current...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N5/078
Inventor 任峰蔡辉国
Owner MEIDE TAIPINGYANG PRECISION INSTR MFG
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