Method for detecting 10 types of aromatic amines compounds in cigarette mainstream smoke by liquid chromatography-tandem mass spectrometry

A technology of cigarette mainstream smoke and tandem mass spectrometry, which is applied in the field of detection of 10 kinds of aromatic amine compounds in cigarette mainstream smoke by liquid chromatography-tandem mass spectrometry. The effect of sample amount and smoking test time and high sensitivity

Active Publication Date: 2012-07-25
ZHENGZHOU TOBACCO RES INST OF CNTC
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  • Abstract
  • Description
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Problems solved by technology

[0007] The purpose of the present invention is to provide a liquid chromatography-tandem mass spectrometry method for detecting aromatic amine compounds in cigarette mainstream smoke in view of the cumbersome and time-consuming pretreatment of the determination method of aromatic amine compounds in the prior art

Method used

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  • Method for detecting 10 types of aromatic amines compounds in cigarette mainstream smoke by liquid chromatography-tandem mass spectrometry
  • Method for detecting 10 types of aromatic amines compounds in cigarette mainstream smoke by liquid chromatography-tandem mass spectrometry
  • Method for detecting 10 types of aromatic amines compounds in cigarette mainstream smoke by liquid chromatography-tandem mass spectrometry

Examples

Experimental program
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Effect test

Embodiment 1

[0042] The balanced and screened cigarettes were smoked according to the standard conditions specified in YC / T29-1996, and the particulate matter in the mainstream smoke of 5 cigarettes was collected with Cambridge filters, and collected in an absorption bottle containing 25 mL of 5% HCl solution Gas phase.

[0043]Fold the Cambridge filter disc for trapping particulate matter into a 50 mL Erlenmeyer flask, pour into the 5% HCl solution in the absorption flask, add the mixed internal standard (D5-aniline, D7-1-aminonaphthalene and D9-4 -Aminobiphenyls were 50 ng / mL, 2.5 ng / mL, 0.5 ng / mL), ultrasonically extracted at room temperature for 30 min, the extraction solution was neutralized with NaOH solid to pH>13, and the 20 Load 1 mL of the extract onto an Agilent Oasis® HLB SPE cartridge that has been activated with 10 mL of methanol solution, allowing the extract to remain naturally by gravity, keeping the flow rate at about 1-1.5 mL / min. Then it was washed with 10 mL of water,...

Embodiment 2

[0046] The Kentucky reference cigarette 3R4F was smoked according to the standard conditions stipulated in YC / T 29-1996. The particulate phase of 5 cigarettes was collected by the Cambridge filter, and the gas phase was collected by the absorption bottle filled with 25 mL of 5% HCl solution. Put the filter in the Erlenmeyer flask, pour into the 5% HCl solution in the absorption flask, add 50 ng / mL D5-aniline, 2.5 ng / mL D7-1-aminonaphthalene and 0.5 ng / mL D9-4-amino Biphenyl was mixed with the internal standard solution, and then the sample was purified and analyzed according to the steps described in Example 1. Five parallel experiments were carried out, and the relative standard deviation of the determination of each compound was between 3.23% and 9.46%, which indicated that the method had good reproducibility and stability.

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Abstract

A method for detecting 10 types of aromatic amines compounds (including aniline, ortho toluidine, m-toluidine, p-aminotoluene, m-phenylenediamine, m-methoxyaniline, 1- naphthylamine, 2- naphthylamine, 3- aminobphenyl and 4- aminobphenyl) in cigarette mainstream smoke by liquid chromatography-tandem mass spectrometry is characterized in that a Cambridge filter and an absorbing bottle containing 5%of HC1 solution respectively gather the aromatic amines compounds in a particle phase matter and a gas phase matter of mainstream smoke, after the 5%HCI solution is used for extracting the aromatic amines compounds on the filter, the aromatic amines compounds are purified by an HLB (hydrophile-lipophile balance) solid-phase extraction column, and a liquid chromatography-tandem mass spectrometry instrument is used for determining the 10 types of aromatic amines in the mainstream smoke in cigarettes. Compared with an existing GC-MS (gas chromatography-mass spectrometer) analysis method for detecting aromatic amines in mainstream smoke of cigarettes, the method does not need derivatization reaction or liquid-liquid extraction operation, greatly simplifies pretreatment steps of samples, improves analysis efficiency, and can more comprehensively detect harmful aromatic amines compounds in mainstream smoke of cigarettes.

Description

[0001] technical field [0002] The invention belongs to the technical field of analysis and determination of harmful chemical components in smoke, and in particular relates to a liquid chromatography-tandem mass spectrometry method for detecting 10 kinds of aromatic amine compounds in mainstream smoke of cigarettes. [0003] Background technique [0004] Aromatic amines and their derivatives have mutagenic factors, which can cause poisoning through inhalation, ingestion or absorption through the skin. They can cause damage to the human blood system by forming methemoglobin, and can also directly act on liver cells to cause toxic liver disease. damage. Some of the aromatic amine derivatives also have carcinogenic and mutagenic effects, even at low concentrations, they have carcinogenic activity on animals and humans. Aromatic amines were distributed in both tobacco leaves and smoke, but the content in smoke was slightly higher than that in tobacco leaves. Systematic resea...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N30/02G01N30/06
Inventor 余晶晶王昇赵阁秦亚琼丁丽刘克建谢复炜张晓兵
Owner ZHENGZHOU TOBACCO RES INST OF CNTC
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