Method for identifying swill-cooked dirty oil by using nematodes

A technology for gutter oil and nematodes, which is applied in the field of gutter oil identification, can solve the problems of unrefined gutter oil detection, high instrument dependence, and complicated operation, and achieves the effects of easy training and mastery, low cost, and convenient experimental operation

Inactive Publication Date: 2012-07-25
NORTHEAST INST OF GEOGRAPHY & AGRIECOLOGY C A S
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  • Abstract
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  • Application Information

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Problems solved by technology

[0004] The purpose of the present invention is to solve the problem that the conventional waste oil detection and identification method through cholesterol has the disadvantages of complex operatio

Method used

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  • Method for identifying swill-cooked dirty oil by using nematodes
  • Method for identifying swill-cooked dirty oil by using nematodes
  • Method for identifying swill-cooked dirty oil by using nematodes

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specific Embodiment approach 1

[0011] Specific embodiment one: the present embodiment utilizes the method for nematode identification waste oil to realize according to the following steps:

[0012] 1. Mix 17g of agar powder, 2.5g of peptone, and 0.11g of CaCl 2 , 0.12g of MgSO 4 , 3g of NaCl and 25mL of phosphate buffer, add distilled water to make up to 1000ml, then put it into a high-pressure steam sterilizer at 120°C for 20min, then cool down to 50-60°C, and then add 1 ~10ml edible oil to be tested to obtain the culture medium, and then pour it into the petri dish;

[0013] 2. After the medium in the petri dish is solidified for 8-12 hours, 5-10 nematode larvae are inserted for observation. If the larvae grow slowly, they can develop into adults after more than 36 hours and the adults are obviously thin and small, with weakened mobility and motor behavior decline. The edible oil to be tested is not waste oil; if it can develop into adults within 24 hours and the growth and activity are in good conditio...

specific Embodiment approach 2

[0016] Specific embodiment two: the difference between this embodiment and specific embodiment one is the configuration of phosphate buffer in step one: the KH of 13.6g 2 PO 4 and 1.8 g of KOH, distilled water was added to make the volume to 100 mL, and the pH was adjusted to 6.0 to obtain a phosphate buffer. Other steps and parameters are the same as those in Embodiment 1.

specific Embodiment approach 3

[0017] Embodiment 3: The difference between this embodiment and Embodiment 1 or 2 is that in step 1, the temperature is lowered to 55° C., and then 2 ml of edible oil to be tested is added in a sterile environment. Other steps and parameters are the same as those in Embodiment 1 or Embodiment 2.

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Abstract

The invention discloses a method for identifying swill-cooked dirty oil by using nematodes, relating to a method for identifying the swill-cooked dirty oil and solving the problems that a conventional method for identifying the swill-cooked dirty oil by using cholesterol has the defects of complexity in operation, high instrument dependency and higher cost and cannot detect the highly refined swill-cooked dirty oil with low trace amount of specific components in a pertinence way. The method comprises the steps of: I, preparing a culture medium and pouring the culture medium into a culture dish; and II, after the culture medium in the culture dish is solidified, putting nematode larvae into the culture medium for observation, and judging that edible oil to be tested is not the swill-cooked dirty oil if the larvae grow slowly and grow into adults after more than 36h and the adults are obviously thin and small in body type, weakened in action capacity and declined in movement behavior, and judging the edible oil to be tested as the swill-cooked dirty oil if the larvae grow into the adults within 24h and are good in growth and action condition, thereby completing the identification. The method disclosed by the invention can be used for effectively identifying the highly refined swill-cooked dirty oil with low trace amount of the specific components and has the advantages of low requirements on experimental conditions, no need of special high-precision analysis instrument, convenience in operation and low cost.

Description

technical field [0001] The invention relates to a method for identifying waste oil. Background technique [0002] Waste oil generally refers to all kinds of low-quality oils that exist in daily life, such as recycled cooking oil, repeatedly used frying oil, and "edible oil" processed from garbage in urban sewers through filtration, heating, precipitation, and separation. Oil". "Gutter oil" is a kind of extremely poor quality, extremely unsanitary non-edible oil. Once "gutter oil" is eaten, it will destroy people's white blood cells and mucous membrane of the digestive tract, cause food poisoning, and even cause serious consequences of cancer. The phenomenon of waste oil returning to the dining table is common in China and has been widely concerned in recent years, but the identification of waste oil has become a difficult problem. At present, there is no unified standard for the detection of waste oil in China. Based on human senses alone, the color, aroma, and taste of ...

Claims

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Application Information

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IPC IPC(8): G01N33/03
Inventor 王云彪李铸衡徐镜波
Owner NORTHEAST INST OF GEOGRAPHY & AGRIECOLOGY C A S
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