Propolis-adjuvant inactivated vaccine for mink pasteurellosis

A pasteurella and inactivated vaccine technology, applied in antibacterial drugs, antibody medical ingredients, medical preparations containing active ingredients, etc., can solve the problems of high morbidity, easy recurrence, and high mortality

Inactive Publication Date: 2014-12-24
QINGDAO AGRI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Pasteurella in mink is also prevalent in recent years. It has a high morbidity rate and high mortality rate. It is very harmful to the fur animal breeding industry and often causes serious economic losses.
[0003] At present, the main treatment of mink pasteurellosis in my country is antibiotics, but there are certain defects in drug treatment. Once the drug is stopped, it is easy to relapse, and it is easy to produce drug resistance.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0016] Vaccine preparation: this product is to select a strain of Pasteurella multocida C52-17 purchased by the inventor from the bacterial strain room of China Veterinary Drug Supervision Institute as the bacterial strain for producing the vaccine. After resuscitation, the blood agar slant medium was inoculated for 18 hours. After checking for no miscellaneous bacteria, use it as a seed liquid, inoculate the blood agar slant with an appropriate amount of bacterial culture, and incubate at 37°C for 18-24 hours, take the cultured bacteria liquid and add formaldehyde solution to make the final concentration of formaldehyde 0.4%, at 37°C Shaker inactivation for 24 hours.

[0017] Vaccine inactivation test: Take 100 microliters of formaldehyde-inactivated bacterial solution and put it in 3ml liquid medium, take three samples for one culture, and inoculate each sample in three test tubes for five days for inactivation safety inspection. If the inoculated liquid culture medium is st...

Embodiment 2

[0020] Three batches of vaccines prepared in Example 1 were injected into healthy minks in single dose, single dose repetition and overdose, and inoculated into the muscles of the hind limbs. After 14 days of observation, the body temperature and appetite of the inoculated minks were normal, there was no swelling and inflammation at the inoculation site, and no local and systemic reactions occurred: 3 animals were selected for each batch of vaccine, and the skin at the injection site was cut to observe the pathological changes of the injection site after necrosis. There was no abnormal change, and the muscle tissue at the inoculation site of the inner muscle of the hind limb was normal, only the injection site was dark brown, the size of a corn kernel, which was different from the surrounding reddish-brown muscle, and there was no inflammatory reaction at the injection site. The 60-70-day-old mink continued to be observed until 30 days after inoculation, and the mink developed ...

Embodiment 3

[0022] Three batches of mink pasteurellosis propolis inactivated vaccines were used to immunize 20 mice of 16-18g respectively, 10 mice in the experimental group and 10 mice in the control group, 0.2mL / mouse, and 21 days after the immunization, 20 mice in the control group were treated with Pasteurella After challenge and observation for 7 days, 80% of the mice in the three batches of vaccine-immunized groups were protected, and 100% of the mice in the control group were infected.

[0023] Three batches of mink pasteurellosis propolis inactivated vaccine were used to immunize healthy minks aged 2-3 months respectively. Each batch of vaccine was inoculated with 10 minks, 1mL / mink. After 21 days after immunization, 10 minks in the control group were passed with Pasteurella. Intratracheal instillation of challenge virus (5 rats in each group), observation for 7 days, 80% of the minks in the three batches of vaccine immunized groups were protected, and 100% of the minks in the cont...

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PUM

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Abstract

The invention provides a propolis-adjuvant inactivated vaccine for mink pasteurellosis and a method for preparing the propolis-adjuvant inactivated vaccine. The method includes: vaccinating mink original pasteurella onto a blood agar slant, culturing for 24 hours at 37 DEG C, checking purity, using as seed pasteurella liquid vaccinated blood agar matrix, inactivating to obtain an aqueous phase to be fully mixed with propolis adjuvant to obtain the propolis-adjuvant inactivated vaccine for mink pasteurellosis. The propolis-adjuvant inactivated vaccine can effectively prevent mink pasteurellosis, and adverse reactions after vaccination and influences on fur quality can be evidently reduced to create conditions for control of fur-bearing animal diseases.

Description

technical field [0001] The invention relates to an inactivated mink pasteurellosis vaccine with propolis as an adjuvant and a preparation method thereof, belonging to the technical field of veterinary biological products. Background technique [0002] Pasteurellosis is mainly a bacterial hemorrhagic and septic infectious disease of livestock, poultry, wild animals and fur animals caused by Pasteurella multocida and Pasteurella hemolyticus. The disease is widely distributed and occurs all over the world. Pasteurella in mink is also prevalent in recent years. It has a high morbidity rate and a high mortality rate. It is very harmful to the fur animal breeding industry and often causes serious economic losses. [0003] At present, the main treatment for mink pasteurellosis in my country is antibiotics, but there are certain defects in the drug treatment. Once the drug is stopped, it is easy to relapse, and it is easy to produce drug resistance. An effective measure to prevent...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61K39/39A61K39/102A61P31/04
Inventor 单虎张传美秦志华王述柏蔡秀磊张启迪
Owner QINGDAO AGRI UNIV
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