Application for isobenzofuran type compounds in marine biofouling prevention and preparation method thereof

A marine biofouling and marine biological technology, applied to the preparation of isobenzofuran compounds, the application field of isobenzofuran compounds in the prevention of marine biofouling, can solve the destruction of marine ecological environment, natural non-toxic Complicated compound preparation process and other issues, to achieve the effect of high purity, strong inhibition of biofouling activity, and easy industrial production

Inactive Publication Date: 2012-08-01
YUNNAN MINZU UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0007] In order to solve the problem that the traditional anti-marine biofouling antifouling agent in the background technology is easy to cause damage to the marine ecological environment, and the natural non-toxic agent with anti-marine biofouling activity Compound preparation process is relatively complicated, the present invention aims to provide isobenzofuran compounds with strong anti-biofouling activity, environment-friendly, simple preparation process and its application in preventing marine biofouling

Method used

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  • Application for isobenzofuran type compounds in marine biofouling prevention and preparation method thereof
  • Application for isobenzofuran type compounds in marine biofouling prevention and preparation method thereof
  • Application for isobenzofuran type compounds in marine biofouling prevention and preparation method thereof

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Effect test

Embodiment 1

[0040] - Inhibitory activity of Cephalosporium AL031 fungal extract on the attachment of barnacle larvae.

[0041] Preparation of ethyl acetate extract of Cephalosporium AL031 fungal solid fermentation product: using rice, corn flour, bagasse, wheat bran as raw materials, mixing and sterilizing in a ratio of 10: 2: 3: 3 (W / W / W / W) ,cool down. Cephalosporium AL031 fungus was inoculated into the mixture, fermented at 28 °C for 6 days, the strain preservation number was CCTCC: M2010140; cold extraction was performed with industrial ethyl acetate for 3 days in the dark, extracted 3 times, the extract was filtered, reduced Press and concentrate to obtain ethyl acetate extract, which is stored in the dark.

[0042] An experimental model for inhibiting the attachment of barnacle Venus larvae was used (see scientific literature: Xu Y., He H. P., Qian P. Y, et al. Potent antifouling compounds produced by marine streptomyces. Bioresource Technology. 2010,101(4): 1331-1336) tested the a...

Embodiment 2

[0047] —— Preparation of 4,5,6-trihydroxy-7-methyl-isobenzofuran and 4,5,6-trihydroxy-7-methyl-isobenzofuranone.

[0048] The isolation and identification process of the anti-biofouling active components in the solid fermentation products of Cephalosporium AL031 fungi is as follows: figure 1 shown.

[0049] Using rice, corn flour, bagasse, and wheat bran as raw materials, mix sterilization and cooling in a ratio of 10: 2: 3: 3 (W / W / W / W); inoculate the fungal strain named Cephalosporium AL031 In the mixed medium, fermented at 28 °C for 6 days, the strain preservation number is CCTCC: M2010140; the solid fermentation product was extracted with redistilled industrial ethyl acetate in the dark for 3 days, extracted 3 times, combined with the extract, Filter and concentrate the extract under reduced pressure to obtain ethyl acetate extract (40 g), disperse the above-mentioned re-distilled industrial ethyl acetate extract in water, use 10 g of re-distilled industrial ethyl acetate...

Embodiment 3

[0056] ——Determination of anti-barnacle larva attachment activity of compounds 4,5,6-trihydroxy-7-methyl-isobenzofuran and 4,5,6-trihydroxy-7-methyl-isobenzofuranone.

[0057] Using the experimental model of inhibiting the attachment of barnacle Venus larvae (see scientific literature: Xu Y., He H. P., Qian P. Y., et al. Potent antifouling compounds produced by marine streptomyces. Bioresource Technology. 2010,101(4):1331- 1336), to test the anti-barnacle larva attachment activity of compounds. Barnacle barnacle adults (Darwin) were collected from the intertidal zone of Hong Kong (22°19'N, 114°16'E). Put 8 L of filtered seawater in a 12 L polystyrene plastic culture container, then put the adult barnacle barnacles into the container, let it release the larvae, and collect the larvae after 2.5 h. The larvae at this stage are called nonodes Larvae (nauplius), incapable of attachment. The nauplii were placed in a container filled with 8 L of filtered seawater (the pore size of ...

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Abstract

The invention discloses an application for isobenzofuran type compounds in marine biofouling prevention and a preparation method thereof, which belongs to the field of marine fouling organism repellency and mainly solves the problem of marine biofouling. The application for isobenzofuran type compounds in marine biofouling prevention can be used for preventing underwater structure surfaces from being attached and/or fouled by marine organisms. The sobenzofuran type compounds are all obtained from isolation of fungus which is named cephalosporium cordaAL 031 in the strain classification, and the preservation serial number of the fungus strain is CCTCC Number M2010140. Extracts of secondary metabolites of the fungus and two isobenzofuran type compounds are performed a fouling resistance activity screening, the experiment result indicates that the extracts of secondary metabolites of the fungus and two isobenzofuran compounds show high fouling resistance activity, attachment of marine fouling organisms can be inhibited, and the application for isobenzofuran type compounds in marine biofouling prevention and the preparation method thereof can be used for prevention and treatment of marine biofouling and have a good application perspective.

Description

Technical field [0001] The present invention involves the field of marine pollution damage biological evasion, and specifically involves the isopenhebly compounds from the sub -alien products of the bacteria of the Ceposopathy AL031 fungal sub -metabolites in the application of the marine biological pollution and the two types of amparies.Preparation method of compounds. Background technique [0002] Bio -stains refer to anywhere of marine creatures such as algae, crustaceans, etc., including ship shells, rudder, propeller, and other hull attachments, or sea water systems such as sea water inhalation box, engine cooling pipeline, ship carrier and subsidiariesEquipment, or biological agglomeration and scaling formed on moving or fixed facilities such as moving or fixed facilities.In recent years, with the development of shipping, coastal defense, aquaculture, and seaside power plants, the harm of pollution loss of marine life has become more and more serious.At present, the most w...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07D307/87C07D307/88A01P1/00A01P7/04A01P17/00A01P7/00C12P17/04C09D5/16C12R1/745
Inventor 黄相中田凯郭俊明张英杰关小丽朱芸张润芝
Owner YUNNAN MINZU UNIV
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