Method for refining capsanthin by enzyme catalysis, esterification and molecular distillation

A technology of capsanthin and molecular distillation, which is applied in the field of natural organic chemistry, can solve the problems of by-products that cannot be directly used, difficult degassing, high distillation temperature, etc., and achieves the effects of low production cost, simple process and high product content

Active Publication Date: 2013-06-12
SOUTH CHINA UNIV OF TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

This process: (1) After degumming with low-carbon alcohol, it does not need to carry out solvent recovery, but directly participates in the esterification reaction; (2) solves the difficulty of degassing in the molecular distillation process, the distillation temperature is high, and the by-products cannot be directly problem of use

Method used

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  • Method for refining capsanthin by enzyme catalysis, esterification and molecular distillation

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0032] A method of enzymatic esterification molecular distillation refining capsanthin of the present invention (such as figure 1 shown), including the following steps:

[0033] Step 1: Degumming

[0034] (1) 10Kg capsanthin oleoresin and 10kg degumming agent (absolute ethanol) are packed in the jacketed reactor, at 60 ℃, stir with 100 rev / min speeds.

[0035] (2) After stirring evenly, pump the mixed solution into a freezing tank, control the temperature of the freezing tank at 5°C, and let it settle.

[0036] (3) After settling for 5 hours, the freezing liquid is 3×10 4 Under the condition of Pa, the upper part of the freezing tank is sucked into a vacuum filter to obtain a filtrate, and the filtrate is separated by a tubular centrifuge to obtain a degummed pigment solution.

[0037] Step 2: Esterification reaction

[0038] (1) Add the degummed pigment solution into the enzyme reactor, add 50g of lipase (Novozym 435) and 100g of water, mix well, control the reaction temp...

Embodiment 2

[0044] In this embodiment, a method for refining capsanthin by enzyme-catalyzed esterification molecular distillation, except for the following differences, other steps and process conditions thereof are the same as in Example 1:

[0045] (1) The quality of adding capsanthin oleoresin and methanol is 10Kg and 50Kg respectively, and stirs evenly at a speed of 500 rpm at 20°C.

[0046] (2) The temperature of the freezing tank is 3°C, and it is left to settle for 10 hours.

[0047] (3) The temperature of the esterification reaction is 45° C., and the reaction is carried out for 5 hours, and the stirring speed is 150 rpm.

[0048] (4) Dehydration under reduced pressure for 2 hours.

[0049] (5) The temperature of the solvent recovered under reduced pressure is 40°C, and the vacuum degree is 6×10 4 Pa.

[0050] (6) The mass ratio of degumming agent, lipase and water is 100:3:3.

[0051] (7) The lipase is Lipozyme RM IM.

[0052] (8) The primary degassing temperature is 100°C, ...

Embodiment 3

[0056] In this embodiment, a method for refining capsanthin by enzyme-catalyzed esterification molecular distillation, except for the following differences, other steps and process conditions thereof are the same as in Example 1:

[0057] (1) Adding capsanthin oleoresin and volume fraction of 95% ethanol aqueous solution are 10Kg and 100Kg respectively, and stirred evenly at 800 rpm at 35°C.

[0058](2) The temperature of the freezing tank is 0°C, and it is allowed to settle for 20 hours.

[0059] (3) The esterification reaction temperature is 30°C, and the reaction is carried out for 10 hours, and the stirring speed is 300 rpm.

[0060] (4) Dehydration under reduced pressure for 3 hours.

[0061] (5) The solvent recovery temperature under reduced pressure is 50°C, and the vacuum degree is 4×10 4 Pa.

[0062] (6) The mass ratio of degumming agent, lipase and water is 100:10:5.

[0063] (7) The lipase is Lipozyme RM IM.

[0064] (8) The primary degassing temperature is 50°...

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Abstract

The invention discloses a method for refining capsanthin by enzyme catalysis, esterification and molecular distillation. The method comprises the following steps of: mixing chili red oil resin and a degumming agent in a mass ratio of 1:1-1:10, stirring uniformly at the temperature of between 20 and 60 DEG C and at the speed of 100 to 800 revolutions per minute, and standing or intermittently stirring and settling at the temperature of between 0 and 5 DEG C; after settling for 5 to 20 hours, filtering under a vacuum condition to obtain filtrate, and separating the filtrate to obtain a degummedpigment solution; adding lipase and water into the degummed pigment solution, mixing uniformly, and reacting at the temperature of between 30 and 60 DEG C under stirring at the speed of 50 to 300 revolutions per minute; after reacting for 2 to 10 hours, dehydrating for 1 to 3 hours; filtering out the lipase, and reclaiming a solvent from the pigment solution under reduced pressure to obtain a fatty acid ester solution containing the capsanthin; and performing two-stage molecular distillation on the fatty acid ester solution to obtain the capsanthin with high color value. The method has the advantages of simple process, high product content, few insoluble acetone substances and low production cost, and is suitable for industrialized production.

Description

technical field [0001] The invention relates to a production process of high color-value capsanthin, in particular to a method for producing high-color-value capsanthin by utilizing enzyme-catalyzed esterification and molecular distillation. The invention belongs to the field of natural organic chemistry. Background technique [0002] Capsanthin is a high-quality natural carotenoid food coloring. Its main components are capsanthin, capsanthin and capsanthin. It not only has bright color, high color value, strong tinting strength, but also has high safety , has no toxic side effects on the human body, and can prolong the shelf life of simulated food, and is widely used in industries closely related to human health such as food, medicine and cosmetics. [0003] Domestically produced capsanthin is basically extracted with 6# solvent, n-hexane, ethanol and other solvents. The capsanthin produced after concentration and recovery of solvent is a kind of oleoresin, which contains ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C09B61/00C09B67/10C09B67/54C12P7/62
Inventor 吕扬效金伟铮
Owner SOUTH CHINA UNIV OF TECH
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