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Method for preparing low-immunogenicity pig dermal support

An immunogenic, dermal technology, applied in medical science, prostheses, etc., can solve the problems of incomplete removal of cell debris components, imperfect barrier function, slow vascularization rate, etc., and achieve normal tissue structure and cell-free Toxicity, the effect of reducing local scars

Active Publication Date: 2012-08-15
武岩
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In addition, any decellularization method cannot completely remove the cell debris components, which become another source of Xeno-ADM immunogen
In addition, problems such as poor permeability, slow vascularization rate, and imperfect barrier function after Allo-ADM transplantation also exist in Xeno-ADM

Method used

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  • Method for preparing low-immunogenicity pig dermal support
  • Method for preparing low-immunogenicity pig dermal support
  • Method for preparing low-immunogenicity pig dermal support

Examples

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Embodiment Construction

[0077] The preparation method of the low immunogenicity porcine dermal scaffold of the present invention comprises the following steps:

[0078] ①Preparation of "double fault" dermis: a. Pigs were sacrificed for hair removal, their skin and part of subcutaneous tissue were peeled off, disinfected and rinsed;

[0079] b. First cut off the epidermis and papillary dermis layer of the skin, then cut off the subcutaneous tissue and the part of the reticular layer of the dermis connected to it, and keep the reticular layer of the dermis with a thickness of 0.5-0.6mm, which is the "double fault" dermis;

[0080] ② Decellularization treatment: a. Prepare 2.5U / ml neutral protease (Dispase II) solution and 0.5% polyethylene glycol octylphenyl ether (TritonX-100) solution by volume;

[0081] b. First put the "double-faulted" dermis described in step ① in 2.5U / ml Dispase II solution, and act for 24 hours at 4°C. At least 2.5ml of "double-faulted" dermis of 0.5-0.6mm per square centimeter ...

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Abstract

The invention discloses a method for preparing low-immunogenicity pig dermal support. The method comprise the following steps of: (1) preparing double fault derma; (2) performing acellular treatment; (3) performing matrix metalloproteinase-7 treatment; and (4) performing vacuum freeze drying treatment. The low-immunogenicity pig dermal support is manufactured by using pig skin as a raw material, aiming at maintaining low-immunogenicity ingredients of pig dermal collagenous fiber and the like and natural three-dimensional structures based on the low-immunogenicity ingredients, removing (eliminating) high-immunogenicity ingredients such as cell and stromatin (such as laminin and fibronectin), and using means of skin material taking and treatment improvement, acellular method optimization, dematrix protein technique establishment, cross-linking agent optimization, vacuum freeze drying technique and the like. According to the method, the purposes of reducing the pig dermal immunogenicity to the level which ensures that the human body can be tolerated and synchronously maintaining the natural three-dimensional structure of the pig skin so as to guide 'ordered' regeneration and reconstruction of human derma in an orginzation mode of in-situ construction organization engineering are reached; and the problems or the prior art are solved.

Description

technical field [0001] The invention relates to a preparation method of a pig dermis scaffold with low immunogenicity. Background technique [0002] Allogeneic acellular dermal matrix (Allo-ADM) is made from split human cadaver skin slices including epidermis, basement membrane and papillary dermis, which are processed by two or more decellularized methods such as physical, chemical and biological. It retains the weak immunogenic collagen (collagen, Col) and some matrix components such as elastic fiber (elastic fiber), proteoglycan (proteoglycan) and structural glycoproteins (structural glycoproteins), and also retains the natural skin of the dermis. Three-dimensional structure and basement membrane that connects the epidermis to the dermis. Although great progress has been made in the basic research and clinical application of Allo-ADM, and good transplantation results have been achieved in the multidisciplinary application of burns, there are still many basic theoretical ...

Claims

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Application Information

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IPC IPC(8): A61L27/36
Inventor 武岩宋国栋邵阳姜金珠
Owner 武岩
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