Method of using oligonucleotide for chiral drug resolution
A technology of oligonucleotides and chiral drugs, applied in the field of medicine, can solve the problems of unreported separation of chiral drugs, and achieve the effect of safe separation
- Summary
- Abstract
- Description
- Claims
- Application Information
AI Technical Summary
Problems solved by technology
Method used
Examples
Embodiment 1
[0021] A method utilizing oligonucleotides to split chiral drugs, comprising the steps of:
[0022] (1) At pH=7.2, add [(dG-dC) to 1000 μL molar concentration of 0.05 mmol / L 20 ] 2 Add 1 μL of an aqueous solution of copper chloride with a molar concentration of 0.1 mol / L to the solution, and mix well at 20°C to obtain [(dG-dC) 20 ] 2 - copper ion complex solution; [(dG-dC) 20 ] 2 The solvent of the solution is sodium phosphate buffer;
[0023] (2) At pH=7.2, 400 μL of [(dG-dC) prepared in step (1) 20 ] 2 -The copper ion complex solution is mixed with 100 μ L molar concentration of racemic ofloxacin solution of 0.5mmol / L, adsorbed for 20 minutes, the solvent of the racemic ofloxacin solution is sodium phosphate buffer; then the mixed solution Pack into the ultrafiltration centrifuge tube that molecular weight cut off is 3000, centrifugal 20 minutes under 8000rpm, filtrate is a kind of enantiomer solution of the enriched ofloxacin, [(dG-dC) 20 ] 2 - The adsorbate on the...
Embodiment 2
[0026] A method utilizing oligonucleotides to split chiral drugs, comprising the steps of:
[0027] (1) At pH=8.5, add [(dG-dC) to 1000 μL molar concentration of 0.05 mmol / L 10 ] 2 Add 10 μL of zinc sulfate aqueous solution with a molar concentration of 0.5 mol / L to the solution, and mix well at 20°C to obtain [(dG-dC) 10 ] 2 -Zinc ion complex solution; [(dG-dC) 10 ] 2 The solvent of the solution is Tris-hydrochloric acid buffer.
[0028] (2) At pH=8.5, 400 μL of [(dG-dC) prepared in step (1) 10 ] 2 -Zinc ion complex solution mixed with 10 μ L molar concentration of racemic ofloxacin solution of 2mmol / L, adsorbed for 40 minutes, the solvent of ofloxacin solution was Tris-hydrochloric acid buffer solution; then the mixed solution was loaded into In the ultrafiltration centrifuge tube that molecular weight cut-off is 1000, centrifugal 20 minutes under 8000rpm, filtrate is a kind of enantiomer solution of the enriched ofloxacin, [(dG-dC) 10 ] 2 - The adsorbate on the zin...
Embodiment 3
[0031] A method utilizing oligonucleotides to split chiral drugs, comprising the steps of:
[0032] (1) At pH=8.5, add [(dA-dT) to 1000 μL molar concentration of 0.025 mmol / L 400 ] 2 Add 20 μL of magnesium nitrate aqueous solution with a molar concentration of 0.05 mol / L to the solution, mix well at 30°C to obtain [(dA-dT) 400 ] 2 -magnesium ion complex solution; [(dA-dT) 400 ] 2 The solvent of the solution is Tris-hydrochloric acid buffer.
[0033] (2) At pH=8.5, 400 μL of [(dA-dT) prepared in step (1) 400 ] 2 -The magnesium ion complex solution is mixed with 400 μ L molar concentration of racemic ibuprofen solution of 0.15mmol / L, adsorbed for 15 minutes, the solvent of the ibuprofen solution is Tris-hydrochloric acid buffer; The ultrafiltration membrane of 10000, filtrate is a kind of enantiomer solution of the ibuprofen of enrichment, [(dA-dT) 400 ] 2 - The adsorbate on the magnesium ion complex is the other enantiomer of the enriched ibuprofen. The enantiomeric exc...
PUM
Login to View More Abstract
Description
Claims
Application Information
Login to View More