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Composite electrode, sensor, biological fuel cell, preparation method and application

A composite electrode, dehydrogenase technology, applied in battery electrodes, material electrochemical variables, material analysis by electromagnetic means, etc., can solve the problems of slow direct electro-oxidation, easy contamination of electrode surface, high overpotential, etc.

Inactive Publication Date: 2012-08-15
ANHUI NORMAL UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0003] The technical problem to be solved by the present invention is to overcome the shortcomings of the existing electrodes such as slow direct electrooxidation of NADH, high overpotential, electrocatalyst (that is, mediator), easy contamination of the electrode surface, and low sensitivity. A composite electrode that is easy to obtain, cheap, has good biocompatibility, and has good electrocatalytic activity under low overvoltage and its preparation method, and its application in the preparation of composite electrodes loaded with dehydrogenase, the preparation of loaded The composite electrode of oxidase or the application in the sensor for detecting NADH, and provides the sensor for detecting NADH and its preparation method at the same time

Method used

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  • Composite electrode, sensor, biological fuel cell, preparation method and application
  • Composite electrode, sensor, biological fuel cell, preparation method and application
  • Composite electrode, sensor, biological fuel cell, preparation method and application

Examples

Experimental program
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Embodiment 1

[0084] The preparation of embodiment 1CS / GC electrode

[0085] (1) Mix 10 mg of double-layer hollow carbon spheres with 1 mL of N,N-dimethylformamide, and obtain a dispersion by ultrasonication.

[0086] (2) The glassy carbon electrode with a diameter of 3 mm was polished in alumina slurry with a particle size of 0.3 μm and 0.05 μm in order to remove surface dirt, and was ultrasonically cleaned with acetone and deionized water for 3 minutes, respectively. 4 μL of the dispersion prepared in step (1) was drop-coated on the surface of the glassy carbon electrode, and dried under light to remove the solvent to prepare a CS / GC electrode.

Embodiment 2

[0087] The preparation of embodiment 2LDH-CS / GC electrode

[0088]Mix LDH, BSA and phosphate with a pH of 7.0 to obtain the LDH coating solution. The concentration of LDH in the LDH coating solution is 20 mg / mL, and the concentration of BSA is 6.7 mM. Take 8 μL of the LDH coating solution and apply it to the example 1 CS / GC electrode, and then add 2 μL of 40 mM glutaraldehyde solution for cross-linking, and dry in a refrigerator at 4°C to prepare an LDH-CS / GC electrode.

Embodiment 3B

[0089] The preparation of embodiment 3BOD-CS / GC electrode

[0090] Mix BOD, BSA and phosphate with a pH of 7.0 to obtain a BOD coating solution. The concentration of BOD in the BOD coating solution is 20 mg / mL, and the concentration of BSA is 6.7 mM. Take 8 μL of the BOD coating solution and apply it dropwise in the example 1 CS / GC electrode, and then add 2 μL of 40 mM glutaraldehyde solution for cross-linking, and dry in a refrigerator at 4°C to prepare a BOD-CS / GC electrode.

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Abstract

The invention discloses a composite electrode and a preparation method thereof. The preparation method includes (1) uniformly mixing carbon double-shell hollow spheres with solvent to form dispersing agent; and (2) uniformly dripping the dispersing agent on the surface of a glassy carbon electrode and drying. Application of the composite electrode in terms of preparation of a composite electrode loaded with dehydrogenase or oxidase and preparation of a sensor for detecting NADH (nicotinamide adenine diuncleotide hydrogen) is realized. The invention further discloses the sensor for detecting NADH, a preparation method of the sensor, the composite electrode loaded with dehydrogenase or oxidase, a preparation method of the composite electrode loaded with dehydrogenase or oxidase, application of the composite electrode in fields of biosensors and biological fuel cells, a biosensor, a biological fuel cell and a preparation method of the biosensor and the biological fuel cell. The biosensor can detect dehydrogenation substrates corresponding to the dehydrogenase without catalysis of media, and has the advantages of fast response, fine selectivity and high sensitivity and accuracy. The biological fuel cell can work in a physiological environment and can realize electro-catalysis without media.

Description

technical field [0001] The present invention relates to electrode materials, in particular to a composite electrode and its preparation method and application, and more to a composite electrode loaded with dehydrogenase and its preparation method and application in biosensors and biofuel cells, and a composite electrode loaded with oxidase and Its preparation method and application in biofuel cells. Background technique [0002] Nicotinamide adenine dinucleotide (nicotinamide adenine dinucleotide, abbreviated as NAD) is a coenzyme of dehydrogenase and an electron carrier in oxidation. It plays a role in both respiration and photosynthesis. Playing the role of core hub. The reduced state of NAD is NADH and the oxidized state is NAD + . NAD + Through various dehydrogenases, it accepts a hydrogen atom and two electrons from the substrate to become reduced NADH. This reaction can proceed reversibly to achieve electron transfer. NADH participates in the enzymatic reactions ...

Claims

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Application Information

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IPC IPC(8): G01N27/30G01N27/327H01M4/88H01M4/86
CPCY02E60/50
Inventor 高峰尹君郭新颖
Owner ANHUI NORMAL UNIV
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