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Process for preparing protein micropattern on surface of PET (polyethylene glycol terephthalate) base material

A substrate surface, micro-pattern technology, applied in the field of biochemistry and organic polymer material surface interface modification, inorganic chemistry, can solve special requirements, poor fidelity repeatability and other problems, to solve signal transmission problems, improve Stability, effect of preventing rearrangement

Inactive Publication Date: 2013-11-20
WUHAN UNIV OF TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] There are many ways to prepare protein micropatterns on the surface of materials, such as microcontact printing [Appl. Phys. Lett 63(14), 1993: 2002~2004], capillary micromolding [Annu Rev Mater Sci 281, 998: 153~184] , electron beam etching [Sci. Technol. B 16,1998: 3526~3534], etc., but these technologies have their own shortcomings, such as process control, special requirements for material selection, fidelity, and repeatability. Poor

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  • Process for preparing protein micropattern on surface of PET (polyethylene glycol terephthalate) base material
  • Process for preparing protein micropattern on surface of PET (polyethylene glycol terephthalate) base material

Examples

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Effect test

Embodiment 1

[0023] Such as figure 1 Shown, a kind of method that bovine serum albumin micropattern is prepared on PET substrate surface, it comprises the steps:

[0024] 1) Preparation of metallic silver micropatterns:

[0025] Fix the photomask (copper mesh, with a line width of about 20 μm) on the surface of a cleaned PET substrate of 1 cm×1 cm, and place it under a 165 nm ultraviolet lamp for 10 min. The base material after the light is placed in the silver plating solution (the solution is composed of V （还原剂） :V （氧化剂） =1:1, wherein the reducing agent is composed of glucose (12 g / l) and potassium sodium tartrate (3 g / l), the oxidizing agent is silver ammonia solution (21 g / l)), and the surface of the substrate is brightly plated After the silver layer, the substrate was taken out, rinsed 3 times with distilled water, blown dry with nitrogen, and stored in a vacuum desiccator for future use.

[0026] 2) Adsorption of mercapto polyethylene glycol (SHPEG):

[0027] The PET substrate ...

Embodiment 2

[0034] A method for preparing bovine serum albumin micropatterns on the surface of a PET substrate, comprising the steps of:

[0035] (1) Preparation of metallic silver micropatterns:

[0036] Fix the photomask (copper mesh, with a line width of about 20 μm) on the surface of a cleaned PET substrate of 1 cm×1 cm, and place it under a 170 nm ultraviolet lamp for 15 min. The base material after the light is placed in the silver plating solution (the solution is composed of V （还原剂） :V （氧化剂） =1:1, wherein the reducing agent is composed of glucose (15 g / l) and potassium sodium tartrate (6 g / l), the oxidizing agent is silver ammonia solution (24 g / l)), and the surface of the substrate is brightly plated After the silver layer, the substrate was taken out, rinsed 4 times with distilled water, blown dry with nitrogen, and stored in a vacuum desiccator for future use.

[0037] (2) Adsorption of mercapto polyethylene glycol (SHPEG):

[0038] The PET substrate with metallic silver mi...

Embodiment 3

[0045] A method for preparing bovine serum albumin micropatterns on the surface of a PET substrate, comprising the steps of:

[0046] (1) Preparation of metallic silver micropatterns:

[0047] Fix the photomask (copper mesh, with a line width of about 20 μm) on the surface of a cleaned PET substrate of 1 cm×1 cm, and place it under a 185 nm ultraviolet lamp for 20 min. The base material after the light is placed in the silver plating solution (the solution is composed of V （还原剂） :V （氧化剂） =1:1, wherein the reducing agent is composed of glucose (19 g / l) and potassium sodium tartrate (8 g / l), the oxidizing agent is silver ammonia solution (27 g / l)), and the surface of the substrate is brightly plated After the silver layer, the substrate was taken out, rinsed 5 times with distilled water, blown dry with nitrogen, and stored in a vacuum desiccator for future use.

[0048] (2) Adsorption of mercapto polyethylene glycol (SHPEG):

[0049] The PET substrate with metallic silver m...

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Abstract

The invention relates to a process for preparing protein micropattern on the surface of a PET (polyethylene glycol terephthalate) base material, which includes steps of firstly, preparing metal silver micropattern on the surface of the PET base material, soaking the PET base materials with the metal silver micropattern into SHPEG water solution so that a silver-plated area adsorbs SHPEG repelling protein and a non-silver-plated area reserves the property of the base material adsorbing protein, and accordingly, preparing the protein micropattern on the PET base material, and controlling selective adsorption of protein on the surface of the PET base material. The protein micropattern on the surface of the PET base material can be applied to surface biological diagnosis and manufacture of biosensors and biomedical devices and the like and creates a certain foundation for research of tissue engineering. Meanwhile, the protein micropattern on the surface of the PET base material is simple in preparation process, easy in control of preparation steps, low in cost and fine in fidelity and has excellent repeatability.

Description

technical field [0001] The invention relates to the fields of inorganic chemistry, biochemistry and surface interface modification technology of organic polymer materials, in particular to a process for preparing protein micropatterns on the surface of a PET (polyethylene terephthalate) substrate. Background technique [0002] The current research hotspots in the field of materials have shifted from the research on the macroscopic fields such as the mechanical properties of materials to the research on the microscopic properties of the material surface. Focus, the development of many modern technologies comes from the construction and miniaturization of new microstructures. In the current field of life sciences, the information on the interaction between them and biomolecules and the surrounding environment can be obtained by modifying the surface of materials and realizing the micropatterning of the material surface, which is very important for the realization of various mo...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C08J7/00C08L67/02
Inventor 吴仲岿唐红肖李少英杨军
Owner WUHAN UNIV OF TECH