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Platymonas subcordiformis medium formula and platymonas subcordiformis three-level culturing method

A subcardioid flat algae and culture medium formulation technology, applied in the field of marine microalgae cultivation, to achieve the effects of easy operation, low pollution and fast growth

Inactive Publication Date: 2012-08-22
LINYI UNIVERSITY
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AI Technical Summary

Problems solved by technology

The previously used flat algae medium did not add sodium bicarbonate, but my experiments showed that subcardioid algae can use free CO in the air 2 , can also effectively utilize the HCO in the culture medium 3 - as carbon source

Method used

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Effect test

Embodiment Construction

[0015] Culture medium preparation method

[0016] 1 Ferric citrate is difficult to dissolve, you can add a small amount of tap water and warm it to 80-90°C on the stove, and keep stirring until it completely melts;

[0017] 2. During the process of adding nutrient salts, the water needs to be continuously stirred, and various nutrient salts are added in the order provided in the formula to avoid chemical reactions;

[0018] 3 Various nutrient salts can be made into mother liquor with higher concentration first, and then added in sequence;

[0019] 4. Nutrient formula and concentration are the same at each stage of tertiary cultivation.

[0020] A subcardioid culture medium, characterized in that the formula of the subcardioid culture medium is as follows: sodium nitrate 100mg, potassium dihydrogen phosphate 45mg, sodium bicarbonate 800mg, iron citrate 0.2mg, human urine 2ml, improved f / 2 vitamin solution 1ml, sterilized sea water 1000ml. The preparation method of the improv...

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Abstract

The invention provides a platymonas subcordiformis medium formula and a platymonas subcordiformis three-level culturing method. The formula includes 100mg of sodium nitrate, 45mg of monopotassium phosphate, 800mg of sodium bicarbonate, 0.2mg of ferric citrate, 2ml of human urine, 1ml of modified f / 2 vitamin solution, and 1000ml of disinfected seawater. The modified f / 2 vitamin solution is made of0.5mg of vitamin B12, 100mg of vitamin B1 and 0.5mg of vitamin H by volume metering to 1000ml with pure water. Experiment results show that by using the optimized medium and three-level culture to culture platymonas subcordiformis, growth speed is fast, yield is high, cost is low, operation is easy, and the platymonas subcordiformis is less prone to pollution.

Description

technical field [0001] The invention belongs to the field of marine microalgae cultivation, and in particular relates to a subcardioid algae medium formula and a three-stage cultivation method. Background technique [0002] At present, there are many kinds of marine microalgae, fast growth, strong adaptability, and easy cultivation. Microalgae contain some specific high value-added bioactive ingredients, so they are widely used in the fields of food industry, aquaculture, medical beauty, wastewater treatment and bioenergy, and have important economic value. Subcardioid algae is one of the most widely studied and applied microalgae. [0003] Subcardioid planktonic algae is a marine single-cell planktonic algae with strong adaptability, good buoyancy, rapid growth and reproduction, and can synthesize high concentrations of polyunsaturated fatty acids PuFA. It is often used as bait for fish, shrimp and shellfish larvae and Food for bait animals. [0004] At present, the main...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/12C12R1/89
Inventor 王培磊
Owner LINYI UNIVERSITY
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