Kit for determining glycated serum protein

A technology of glycosylated serum protein and kit, which is applied in the field of kits for the determination of glycosylated serum protein, can solve the problems of high cost, small sample size, and unsuitability for clinical routine development, and achieve low cost, simple operation, and wide linear range Effect

Inactive Publication Date: 2012-08-29
AILEX TECH GRP CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Determination of glycosylated serum protein by HPLC can accurately detect the overall level of blood sugar control in patients in the short term, but it was not widely used at that time because of its high cost and small sample size, which was not suitable for clinical routine development

Method used

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  • Kit for determining glycated serum protein
  • Kit for determining glycated serum protein
  • Kit for determining glycated serum protein

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0022] Reagent R1 includes the following components:

[0023] MES buffer 20mmol / L;

[0024] TritonX-100 (surfactant) accounts for 0.5% of reagent R1;

[0025] Sodium periodate (anti-interfering substance) 0.05mmol / L;

[0026] Proteinase K 20KU / L;

[0027] Trehalose (stabilizer) 1g / L;

[0028] Sodium azide (preservative) 0.05g / L;

[0029] Reagent R2 includes the following components:

[0030] Potassium phosphate buffer 20mmol / L;

[0031] Fructose amino acid oxidase (FAOD) 10KU / L;

[0032] Horseradish peroxidase (HRP) 20KU / L;

[0033] Chromogen (DA-64) 0.1mmol / L;

[0034] Gentamicin (preservative) 0.05g / L;

[0035] Trehalose (stabilizer) 1g / L;

[0036] Measure the content of glycosylated serum protein (GSP) in the fresh patient sample with above-mentioned kit, detect on Hitachi 7170 biochemical analyzer, measure parameters as follows:

[0037] Analysis method: two-point endpoint method;

[0038] Measuring temperature: 37°C;

[0039] The light path of the cuvette is ...

Embodiment 2

[0058] Reagent R1 includes the following components:

[0059] Potassium phosphate buffer 20mmol / L;

[0060] TritonX-100 (surfactant) accounts for 0.5% of reagent R1;

[0061] Potassium ferrocyanide (anti-interfering substance) 0.1mmol / L;

[0062] Proteinase K 20KU / L;

[0063] Mannitol (stabilizer) 0.5g / L;

[0064] Gentamicin (preservative) 0.05g / L;

[0065] Reagent R2 includes the following components:

[0066] Potassium phosphate buffer 20mmol / L;

[0067] Fructose amino acid oxidase (FAOD) 10KU / L;

[0068] Horseradish peroxidase (HRP) 20KU / L;

[0069] Chromogen (DA-64) 0.1mmol / L;

[0070] Gentamicin (preservative) 0.05g / L;

[0071] Bovine serum albumin (stabilizer) 0.5g / L;

[0072]The commercially available glycated serum protein concentration is 1500 μmol / L linear quality control substance, and the reagent prepared in Example 2 is used for determination on a Hitachi 7170 automatic biochemical analyzer. The measurement parameters are the same as in Example 1, and th...

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Abstract

The invention relates to a medical reagent, in particular to a kit for determining glycated serum protein, which comprises a reagent R1 and a reagent R2, wherein the reagent R1 contains buffer solution of 20mmol/L, surfactants accounting for 0.5 percent of the reagent R1, anti-interference substances of 0.05-0.1mmol/L, stabilizers of 0.5-1g/L, and preservatives of 0.05g/L. The reagent R2 contains fructosyl amino acid oxidase of 20mmol/L, horseradish peroxidase of 20KU/L, chromogen of 0.1mmol/L, preservatives of 0.05g/L and stabilizers of 0.5-1g/L. Compared with the prior art, when the kit for determining glycated serum protein is used, the glycated serum protein can be determined in an enzymatic method, the HPLC (high performance liquid chromatography) can be completely substituted, and the kit can be applied to clinical laboratories for determination of large bulk samples and can be operated easily. Moreover, the kit has a wide linear scope and high value of application, and the cost of the kit is low.

Description

[technical field] [0001] The invention relates to a medical reagent, in particular to a kit for measuring glycosylated serum protein. [Background technique] [0002] Glycosylated serum protein (GSP) is one of the indicators for clinical blood glucose monitoring, and it has high clinical value for evaluating the recent blood glucose control of diabetic patients and reflecting changes in blood glucose levels in the short term. [0003] In the human body, glucose and protein undergo a non-enzymatic glycation reaction to form glycated protein. Glycosylated hemoglobin (HbA1c) is a glycoprotein formed by the non-enzymatic reaction between glucose in the blood and hemoglobin in red blood cells within 120 days of its life, so it can reflect the overall blood sugar level of the patient within 2 to 3 months before the measurement. It is an international The gold standard for evaluating long-term blood sugar control in the world, because of the long half-life of hemoglobin, its measur...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N21/31
Inventor 李子樵孙明礼
Owner AILEX TECH GRP CO LTD
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