Ethanol conversion method by utilization of marine sulfating carragheenan
A technology of acid-based carrageenan and acid-based carrageenan, which is applied in the field of marine biology to achieve the effects of low production cost, simple method and high enzyme digestion rate
Inactive Publication Date: 2012-09-12
OCEAN UNIV OF CHINA
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However, in view of the fact that the research results on the degradation of carrageenan are limited to the preparation of oligosaccharides rather than fermentable reducing sugars, so far, the method of developing bioethanol from marine algae polysaccharides (carrageenan polysaccharides) has not been reported. The research and development of matter energy will have a broad prospect
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[0029] (1) Preparation of the substrate solution: desulfated carrageenan obtained by chemically modifying the carrageenan polysaccharide was used as the substrate sample, rotary steamed at 60°C for 30 minutes, and centrifuged at 3000r / min for 10 minutes to remove the precipitate to obtain the sample solution; Determine the concentration of the polysaccharide solution by the sulfuric acid method, put the sample solution into the enzymolysis device, add water to prepare a desulfated carrageenan solution with a substrate concentration of 0.5%;
[0030] (2) Add 0.2M HCl or 0.2M NaCl solution to the desulfated carrageenan solution to adjust the pH of the substrate solution system to 4.0;
[0031] (3) Enzymatic hydrolysis system: add 40u / mg substrate pectinase solution to the substrate solution, shake it well, control the temperature to 50°C, carry out saccharification enzymolysis in a water bath for 72 hours, and the yield of reducing sugar reaches 70%;
[0032] The specific calcul...
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The invention relates to an ethanol conversion method by the utilization of marine sulfating carragheenan. An ethanol fermenting material provided by the invention is abundant and requires low cost. The method provides reliable and stable glycolysis sugar for complete enzymatic hydrolysis and conversion into ethanol for fermentation. Degraded products will not inhibit ethanol conversion in the later stage. Efficiency of the method provided by the invention is remarkably raised in comparison with efficiency of a traditional ethanol conversion method. The method provided by the invention comprises the following steps of: firstly using a sulfating carragheenan solution obtained after glycolysis modification as a substrate, adding water to prepare a substrate solution with a certain concentration, adjusting the system to a proper pH value, controlling the temperature of the system, adding pectase to prepare glycolysis sugar under the condition of 50 DEG C, condensing an enzymatic saccharified liquid obtained after the reaction to a certain concentration, adjusting the system to a proper pH value, supplementing essential inorganic salt, inoculating microzyme into a reaction solution, controlling the temperature of the system, and carrying out anaerobic fermentation to prepare ethanol. Through the above technology, ethanol conversion is finally realized by saccharification and fermentation of marine sulfating carragheenan.
Description
technical field [0001] The invention relates to the technical field of marine organisms, in particular to an ethanol conversion method of marine desulfated carrageenan. Background technique [0002] With the rapid development of the world economy, the depletion of fossil energy and environmental pollution have become increasingly prominent. It is imminent to implement energy diversification and develop new renewable energy. Due to its renewability and environmental friendliness, bioethanol has received unprecedented attention from various countries, and is considered to be one of the modern energy sources that can be transformed into high-quality through technology. The current bioethanol is mainly produced by using crops (corn, sugarcane, potato, plant cellulose straw, agricultural processing by-products and shrub energy forest cellulose) as raw materials, which greatly affects the food security of the world population. In order to get rid of the embarrassing situation of ...
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IPC IPC(8): C12P7/06C12R1/865
CPCY02E50/10
Inventor 王鹏牟海津李晓琳
Owner OCEAN UNIV OF CHINA