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Collecting liquid capable of keeping activity of maggot secretion excrements

A technology for excrement and liquid collection, which is applied to medical preparations containing active ingredients, preparation methods of peptides, medical raw materials derived from arthropods, etc. The stability of other biologically active substances, affecting the overall quality of grain worm secretion and excrement, etc., achieve the effect of ensuring quality, beneficial to therapeutic efficacy, and beneficial to clinical application

Inactive Publication Date: 2012-09-19
FIRST AFFILIATED HOSPITAL OF DALIAN MEDICAL UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

It is difficult to maintain the stability of protein and other biologically active substances in the excretions of grain worms with physiological saline as the collection solution, thus affecting the overall quality of the excretions of the collected grain worms and not conducive to long-term storage, which restricts the secretion and excretion of grain worms Further research on the clinical application and therapeutic efficacy of drugs

Method used

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  • Collecting liquid capable of keeping activity of maggot secretion excrements
  • Collecting liquid capable of keeping activity of maggot secretion excrements

Examples

Experimental program
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Effect test

Embodiment 1

[0008] Dissolve 10 grams of Tween 80 in 1 liter of phosphate buffer to obtain a collection solution.

[0009] Both the Tween 80 and the phosphate buffer saline (PBS) are prior art.

[0010] The collection process is the same as the prior art, that is, put the cleaned grain worms into the collection solution (20 pieces / 10ml) of this embodiment 1 for about 5-10 minutes, take out the grain worms, and then the collected liquid contains the grain worms that are spit out and excreted. secrete excrement.

Embodiment 2

[0012] The collected solution was obtained by dissolving 1 gram of bovine serum albumin (BSA) powder in 1 liter of phosphate buffered saline.

[0013] Both the bovine serum albumin (BSA) powder and the phosphate buffer saline (PBS) are prior art.

[0014] The collection process is the same as that of the prior art, that is, put the cleaned grain worms into the collection liquid (20 pieces / 10ml) of this embodiment 2 for about 5-10 minutes, take out the grain worms, and the collected liquid contains the grain worms that are spit out and excreted. secrete excrement.

[0015] experiment:

[0016] Take embodiment 1 of the present invention, embodiment 2 and prior art (physiological saline) as the collection that contains the excreta secreted by grain insects obtained by collecting liquid, and perform gel electrophoresis after repeated freezing and thawing 10 times under the same conditions, electrophoresis Figure such as figure 1 Shown: 1 is the embodiment 1 of the present inven...

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Abstract

The invention discloses a collecting liquid capable of keeping the activity of maggot secretion excrements, which is phosphate buffer solution of Tween80 or phosphate buffer solution of serum albumin, the mass-volume ratio of the Tween80 to the phosphate buffer solution is 10 gram / liter, and the mass-volume ratio of the serum albumin to the phosphate buffer solution is 1 gram / liter. The collecting liquid has the benefits that biological active components of the collected maggot secretion excrements can be kept stable, the quality of collected matters is ensured, the maggot secretion excrements can be still kept in stability even if the temperature is more largely changed (the number of the repeated freezing and thawing times reaches 10) and can be saved for a long time, and the clinical application of the maggot secretion excrements and the further research on treatment efficacy are facilitated.

Description

technical field [0001] The invention relates to a collection liquid for collecting excrement secreted by grain worms, in particular to a collection liquid capable of maintaining the stability of biologically active components in the excreted excretion and facilitating preservation of the excreta secreted by grain insects. Background technique [0002] Since grain worms (maggots) are able to devour / gnaw carrion to promote wound healing, live grain worms have long been used to treat necrotic and heavily infected wounds caused by trauma. At present, it has been found that the secretions and excreta in the mouth of grain insects contain a large number of biologically active components, such as proteins and polypeptide compounds, which play an important role in antibacterial, anti-inflammatory and promoting cell proliferation. The existing method of collecting excrement secreted by grain worms is: put the washed grain worms into physiological saline (20 pieces / 10ml) for about 5-1...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07K1/00A61K35/24A61K35/64
Inventor 吕德成李培楠李宏王寿宇孔庆友于丽君张振
Owner FIRST AFFILIATED HOSPITAL OF DALIAN MEDICAL UNIV