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Separation chamber device used for preparative free-flow electrophoresis

A preparation type and separation chamber technology, which is applied in the preparation of test samples, separation methods, and separation of dispersed particles, etc., can solve the problems of reduced FFE operation efficiency, low effective utilization of voltage, and poor sealing of separation chambers, etc., to achieve Improved electrical conductivity, easy production, and strong electrical conductivity

Inactive Publication Date: 2012-09-26
SHANGHAI JIAO TONG UNIV
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Problems solved by technology

However, the preparation of this polyacrylamide membrane before electrophoresis operation is quite tedious, and the separation chamber is not well sealed, which reduces the operating efficiency of FFE
Moreover, the inherent properties of this membrane make the life of this electrophoretic separation chamber very short
In addition, the electrical conductivity of this film material is average, resulting in a relatively low effective voltage utilization.

Method used

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  • Separation chamber device used for preparative free-flow electrophoresis
  • Separation chamber device used for preparative free-flow electrophoresis
  • Separation chamber device used for preparative free-flow electrophoresis

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Embodiment Construction

[0041] The technical solution of the present invention will be further described below in conjunction with the accompanying drawings, but the protection scope of the present invention should not be limited thereby.

[0042] Please see first figure 1 , figure 1 It is a schematic structural view of the separation chamber device used for free-flow electrophoresis in the present invention, as shown in the figure, between the first organic glass flat plate 1 and the second organic glass flat plate 3 are hollowed ion exchange membranes 2 and plastic films 4, three The latter adopts the "sandwich" method to jointly form the electrophoretic separation chamber (separation hole 21) and the electrode chamber (electrode hole 22). The plastic screws 5 that are evenly distributed everywhere in the periphery run through the first plexiglass flat plate 1, the plastic film 4 and the second plexiglass flat plate 3 successively, so as to achieve the effect of sealing. The bottom center of th...

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Abstract

A separation chamber device used for preparative free-flow electrophoresis (FFE) comprises a first synthetic glass flat plate, a second synthetic glass flat plate, an electrophoresis chamber and an electrode chamber; the electrophoresis chamber and the electrode chamber, which are formed by a separation hole and an electrode hole of an ion-exchange membrane, and a plastic film respectively, are clamped between the first and the second synthetic glass flat plates; a plastic screw passes through an upper screw hole on the first synthetic glass flat plate, a lower screw hole on the second synthetic glass flat plate and a film screw hole of the plastic film to be fixed; and an electrode buffer solution inlet and an electrode buffer solution outlet are formed on the first synthetic glass flat plate and at the positions corresponding to two ends of the electrode hole of the ion-exchange membrane and are used for circulating electrode solution. The invention can solve the problems of leak tightness and stability of the traditional FFE, provides a high-stability and high-uniformity hydrodynamics environment, is extensively applied to conventional FFE separation of various samples such as nucleic acid / protein / polypeptide / virus, and can be applied to various sample purifications carried out in FFE devices.

Description

technical field [0001] The present invention relates to the technical field of biochemical devices, in particular to a separation chamber device for preparative free-flow electrophoresis (hereinafter referred to as FFE). Background technique [0002] Because electrophoresis (Electrophoresis) technology can separate many biomolecules, such as proteins, peptides and cells, it has been widely used in the field of biochemistry. With the development of electrophoresis technology, the supporting medium in the electrophoresis process is gradually replaced by gel from the initial filter paper and cellulose acetate membrane. The introduction of gel as a supporting medium has greatly promoted the development of electrophoresis technology, making electrophoresis technology one of the important means for analyzing biological macromolecules such as proteins and nucleic acids. However, electrophoresis filled with solid media has many disadvantages. First of all, the content of the proce...

Claims

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Application Information

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IPC IPC(8): B01D57/02G01N1/34
Inventor 曹成喜杨经华郭陈刚王后禹樊柳荫杨成章孔凡志
Owner SHANGHAI JIAO TONG UNIV
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