Method for producing low-protein concentrated natural latex by compound proteinase enzymolysis technology

A compound protease and natural rubber latex technology, which is applied in the field of producing low-protein concentrated natural latex by using compound protease enzymatic hydrolysis technology, can solve problems affecting the deproteinization effect of concentrated natural latex, differences in stability and processing characteristics, and affecting protease activity. The dosage of enzyme, the effect of stable product quality and low cost of raw materials

Inactive Publication Date: 2012-10-03
YUNNAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The common method of inactivating enzymes is: at 70 o C for 3h or at 85 o The condition of C has been processed for 10 minutes, so the problems are that the equipment investment is large, the energy consumption is high, and the operation is difficult.
On the other hand, enzyme activity may affect protease activity due to temperature factors
In these production methods, the main problem is that the activity of the enzyme is different under different temperature conditions, which will affect the deproteinization effect of the concentrated natural rubber latex, and will also lead to differences in stability and processing characteristics.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0017] Get 100kg of fresh latex, add 200g of ammonium laurate stabilizer, stir for 2h, at 25 o Under the temperature condition of C, add 5g of alkaline protease 2709 (dissolved in water) to the fresh latex, and stir for 8h; add the anionic surfactant sodium lauryl sulfate with a mass fraction of 0.09% in the above enzymolysis reaction, and stir for 3h , add 5g proteinase K to fresh latex, at 30 o React at a temperature of C for 7 hours; when the nitrogen content of the concentrated latex drops to less than 0.12% by mass, add 30 g of ammonium laurate, and after centrifugal concentration, supplement ammonia to 0.07% (mass percentage), and finally add 7 g of ammonium laurate to the concentrated milk EDTA to inhibit enzyme activity and preserve. Obtain 16.8kg of low-protein natural concentrated latex with a nitrogen content of 0.11%.

Embodiment 2

[0019] Get 100kg fresh latex, add 200g ammonium laurate stabilizer, stir for 2h, o Under the temperature condition of C, add 8g of alkaline protease 2709 (dissolved in water) to the fresh latex, and stir for 8h; add the anionic surfactant sodium lauryl sulfate with a mass fraction of 0.09% in the above enzymolysis reaction, and stir for 3h , add 10g proteinase K to fresh latex, at 37 o React at a temperature of C for 7 hours; when the nitrogen content of the concentrated latex drops to less than 0.12% by mass, add 30 g of ammonium laurate, and after centrifugal concentration, supplement ammonia to 0.07% (mass percentage), and finally add 8 g of ammonium laurate to the concentrated milk EDTA to inhibit enzyme activity and preserve. Obtain 17.2 kg of low-protein natural concentrated latex with a nitrogen content of 0.09%.

Embodiment 3

[0021] Take 100kg fresh latex, add 200g ammonium laurate stabilizer, stir for 2h, o Under the temperature condition of C, add 7g of alkaline protease 2709 (dissolved in water) to the fresh latex, and stir for 8h; add the anionic surfactant sodium lauryl sulfate with a mass fraction of 0.09% in the above enzymolysis reaction, and stir for 3h , add 9g proteinase K in fresh latex, at 37 o React at a temperature of C for 7 hours; when the nitrogen content of the concentrated latex drops to less than 0.12% by mass, add 30 g of ammonium laurate, and after centrifugal concentration, supplement ammonia to 0.07% (mass percentage), and finally add 8 g of ammonium laurate to the concentrated milk EDTA to inhibit enzyme activity and preserve. 17.7 kg of low-protein natural concentrated latex was obtained with a nitrogen content of 0.10%.

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Abstract

The invention relates to a method for producing low-protein concentrated natural latex by a compound enzyme method. The method is characterized in that lauric acid ammonium is added into fresh latex for carrying out stabilizing treatment, the stirring is carried out for 2 to 4 hours, under the condition with the temperature being 20 to 40 DEG C, 0.02 to 0.1 g of alkali protease 2709 is added in per kilogram of fresh latex, and the stirring lasts 5 to 10 hours; anionic surfactants of lauryl sodium sulfate with the mass percent being 0.07 percent to 0.14 percent are added into the enzymolysis reaction, the stirring is carried out for 2 to 5 hours, 0.02 to 0.2g of protease K is added into per kilogram of fresh latex, and the stirring is carried out for 6 to 12 hours at the temperature being 20 to 40 DEG C; and when the nitrogen content of the latex to be concentrated is lower than 0.12 mass percent, the lauric acid ammonium is added in a supplementary way after centrifugal concentration, finally, EDTA (ethylene diamine tetraacetic acid) accounting for 0.005 percent to 0.010 percent of the total mass of the concentrated latex is added in the concentrated latex in time for inhibiting enzyme activity, and the materials are stored.

Description

technical field [0001] The invention belongs to a method for producing low-protein concentrated natural rubber latex by using compound protease enzymolysis technology, and specifically relates to the staged stabilization treatment of natural latex, the use of compound protease to decompose the protein in natural rubber particles, and the removal of protein and enzymes in natural latex Activity Inhibition Technology. Background technique [0002] The demand for latex products at home and abroad is increasing at a rate of 5% to 10% every year, and its market capacity will become larger and larger. However, since natural rubber contains a considerable amount of protein, it increases the hygroscopicity, electrical conductivity and heat generation of natural rubber products, especially causing allergies in contact with natural rubber products, thus limiting natural rubber products with high protein content Application in medical, health care and electrical insulation products. ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C08C1/04C08L7/02
Inventor 李灿鹏赵卉和智坤欧灵澄
Owner YUNNAN UNIV
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